دورية أكاديمية
lncRNA- RP11-156p1.3, novel diagnostic and therapeutic targeting via CRISPR/Cas9 editing in hepatocellular carcinoma.
| العنوان: | lncRNA- RP11-156p1.3, novel diagnostic and therapeutic targeting via CRISPR/Cas9 editing in hepatocellular carcinoma. |
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| المؤلفون: | Ali HS; The department of medicinal biochemistry and molecular biology, The school of Medicine, University of Ain Shams, Egypt., Boshra MS; The department of medicinal biochemistry and molecular biology, The school of Medicine, University of Ain Shams, Egypt., El Meteini MS; Department of General Surgery, the school of Medicine, University of Ain Shams, Abbassia, Cairo, Egypt., Shafei AE; Faculty of Medicine, Modern University for Technology & Information, Cairo, Egypt., Matboli M; The department of medicinal biochemistry and molecular biology, The school of Medicine, University of Ain Shams, Egypt. Electronic address: drmarwa_matboly@med.asu.edu.eg. |
| المصدر: | Genomics [Genomics] 2020 Sep; Vol. 112 (5), pp. 3306-3314. Date of Electronic Publication: 2020 Jun 13. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 8800135 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1089-8646 (Electronic) Linking ISSN: 08887543 NLM ISO Abbreviation: Genomics Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: San Diego : Academic Press, [c1987- |
| مواضيع طبية MeSH: | Carcinoma, Hepatocellular/*genetics , RNA, Long Noncoding/*metabolism, Biomarkers, Tumor/blood ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/metabolism ; CRISPR-Cas Systems ; Carcinoma, Hepatocellular/diagnosis ; Carcinoma, Hepatocellular/metabolism ; Carcinoma, Hepatocellular/therapy ; Female ; Gene Editing ; Hep G2 Cells ; Humans ; Liver/metabolism ; Liver Neoplasms/diagnosis ; Liver Neoplasms/genetics ; Liver Neoplasms/therapy ; Male ; Membrane Proteins/blood ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; MicroRNAs/blood ; MicroRNAs/metabolism ; Middle Aged ; RNA, Long Noncoding/blood ; RNA, Messenger/blood ; RNA, Messenger/metabolism |
| مستخلص: | We aim to characterize the expression of RNA panel in HCC. We assessed the expression of HCC-associated mRNA, miRNA and lncRNA network by real time PCR in sera and tissue samples. In a proof-of-principle approach, CRISPR cas9 mediated knock out for lncRNA- RP11-156p1.3 was performed in HEPG2 cell line to validate the role of the chosen RNA in HCC pathogenesis. The differential expression of RFTN1 mRNA, lncRNA- RP11-156p1.3 and miRNA-4764-5p was statistically different among the studied groups. After CRISPR cas9 mediated knockout of lncRNA- RP11-156p1.3 in HEPG2 cells, there was significant decrease in cell count and viability with reversal of the expression of the chosen RNAs. The chosen RNAs play a significant role in HCC pathogenesis and may be potential diagnostic and therapeutic targets. Competing Interests: Declaration of Competing Interest None. (Copyright © 2020 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: CRISPR cas9,Diagnosis; Hepatocellular carcinoma; Therapy; lncRNA; miRNA |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) 0 (Membrane Proteins) 0 (MicroRNAs) 0 (RFTN1 protein, human) 0 (RNA, Long Noncoding) 0 (RNA, Messenger) |
| تواريخ الأحداث: | Date Created: 20200617 Date Completed: 20210816 Latest Revision: 20210816 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.ygeno.2020.06.020 |
| PMID: | 32544548 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1089-8646 |
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| DOI: | 10.1016/j.ygeno.2020.06.020 |