دورية أكاديمية
Neural In Vitro Models for Studying Substances Acting on the Central Nervous System.
| العنوان: | Neural In Vitro Models for Studying Substances Acting on the Central Nervous System. |
|---|---|
| المؤلفون: | Fritsche E; IUF-Leibniz Research Institute for Environmental Medicine at the Heinrich-Heine-University Dusseldorf gGmbH, Dusseldorf, Germany. ellen.fritsche@iuf-duesseldorf.de., Tigges J; IUF-Leibniz Research Institute for Environmental Medicine at the Heinrich-Heine-University Dusseldorf gGmbH, Dusseldorf, Germany., Hartmann J; IUF-Leibniz Research Institute for Environmental Medicine at the Heinrich-Heine-University Dusseldorf gGmbH, Dusseldorf, Germany., Kapr J; IUF-Leibniz Research Institute for Environmental Medicine at the Heinrich-Heine-University Dusseldorf gGmbH, Dusseldorf, Germany., Serafini MM; Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, Italy., Viviani B; Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, Italy. barbara.viviani@unimi.it. |
| المصدر: | Handbook of experimental pharmacology [Handb Exp Pharmacol] 2021; Vol. 265, pp. 111-141. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 7902231 Publication Model: Print Cited Medium: Print ISSN: 0171-2004 (Print) Linking ISSN: 01712004 NLM ISO Abbreviation: Handb Exp Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin, New York, Springer-Verlag. |
| مواضيع طبية MeSH: | Induced Pluripotent Stem Cells* , Neurotoxicity Syndromes*, Animals ; Cell Differentiation ; Central Nervous System ; Humans ; Neurons |
| مستخلص: | Animal models have been greatly contributing to our understanding of physiology, mechanisms of diseases, and toxicity. Yet, their limitations due to, e.g., interspecies variation are reflected in the high number of drug attrition rates, especially in central nervous system (CNS) diseases. Therefore, human-based neural in vitro models for studying safety and efficacy of substances acting on the CNS are needed. Human iPSC-derived cells offer such a platform with the unique advantage of reproducing the "human context" in vitro by preserving the genetic and molecular phenotype of their donors. Guiding the differentiation of hiPSC into cells of the nervous system and combining them in a 2D or 3D format allows to obtain complex models suitable for investigating neurotoxicity or brain-related diseases with patient-derived cells. This chapter will give an overview over stem cell-based human 2D neuronal and mixed neuronal/astrocyte models, in vitro cultures of microglia, as well as CNS disease models and considers new developments in the field, more specifically the use of brain organoids and 3D bioprinted in vitro models for safety and efficacy evaluation. |
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| فهرسة مساهمة: | Keywords: Bioprinted neuronal models; Brain organoids; CNS disease models; Developmental neurotoxicity (DNT); Human induced pluripotent stem cells (hiPSCs); Microglia culture; Neurotoxicity (NT) |
| تواريخ الأحداث: | Date Created: 20200629 Date Completed: 20210329 Latest Revision: 20210329 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1007/164_2020_367 |
| PMID: | 32594299 |
| قاعدة البيانات: | MEDLINE |
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