دورية أكاديمية
أعرض في EDS

Quantitative immunohistochemical analysis of myeloid cell marker expression in human cortex captures microglia heterogeneity with anatomical context.

التفاصيل البيبلوغرافية
العنوان: Quantitative immunohistochemical analysis of myeloid cell marker expression in human cortex captures microglia heterogeneity with anatomical context.
المؤلفون: Swanson MEV; Department of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.; Centre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand., Murray HC; Department of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.; Centre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand., Ryan B; Department of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.; Centre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand., Faull RLM; Department of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.; Centre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand., Dragunow M; Centre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand.; Department of Pharmacology and Clinical Pharmacology, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand., Curtis MA; Department of Anatomy and Medical Imaging, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand. m.curtis@auckland.ac.nz.; Centre for Brain Research, Faculty of Medical and Health Science, University of Auckland, Private Bag 92019, Auckland, New Zealand. m.curtis@auckland.ac.nz.
المصدر: Scientific reports [Sci Rep] 2020 Jul 16; Vol. 10 (1), pp. 11693. Date of Electronic Publication: 2020 Jul 16.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Fluorescent Antibody Technique/*methods , Microglia/*metabolism , Myeloid Cells/*metabolism , Temporal Lobe/*metabolism, Aged ; Aged, 80 and over ; Antigens, CD/metabolism ; Antigens, Differentiation, Myelomonocytic/metabolism ; Apoferritins/metabolism ; Autopsy ; Biomarkers/metabolism ; Female ; HLA-DR Antigens/metabolism ; Humans ; Macrophages/metabolism ; Male ; Membrane Glycoproteins/metabolism ; Middle Aged ; Receptors, Cell Surface/metabolism ; Receptors, IgG/metabolism ; Receptors, Immunologic/metabolism
مستخلص: Current immunohistochemical methods of studying microglia in the post-mortem human brain do not capture the heterogeneity of microglial function in response to damage and disease. We therefore investigated the expression of eight myeloid cell proteins associated with changes in function alongside Iba1. To study the myeloid cells we used immunohistochemistry on post-mortem human middle temporal gyrus sections from neurologically normal individuals. First we investigated co-labelling between the classical 'activation' marker, HLA-DR and each of the other markers of interest. Significant co-labelling between HLA-DR with CD206, CD32, CD163, or L-Ferritin was observed, although complete overlap of expression of HLA-DR with aforementioned markers was not observed. A qualitative assessment also demonstrated that perivascular macrophages expressed higher levels of the markers of interest we investigated than microglia, suggesting perivascular macrophages show a more phagocytic and antigen presentation state in the human brain. To determine whether the markers of interest were expressed in different functional states, the immunoreactivity for each marker was qualitatively assessed on microglial morphologies. Degenerating marker, L-Ferritin, was specific for dystrophic microglia. We demonstrate that microglial heterogeneity can be investigated in immunohistochemically stain post-mortem human tissue by integrating the single-cell abundance of proteins and cell morphology to infer function.
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المشرفين على المادة: 0 (Antigens, CD)
0 (Antigens, Differentiation, Myelomonocytic)
0 (Biomarkers)
0 (CD163 antigen)
0 (FCGR2B protein, human)
0 (HLA-DR Antigens)
0 (MRC1 protein, human)
0 (Membrane Glycoproteins)
0 (Receptors, Cell Surface)
0 (Receptors, IgG)
0 (Receptors, Immunologic)
9013-31-4 (Apoferritins)
تواريخ الأحداث: Date Created: 20200718 Date Completed: 20210107 Latest Revision: 20210716
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7366669
DOI: 10.1038/s41598-020-68086-z
PMID: 32678124
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-020-68086-z