دورية أكاديمية
Inversion Genotyping in the Anopheles gambiae Complex Using High-Throughput Array and Sequencing Platforms.
| العنوان: | Inversion Genotyping in the Anopheles gambiae Complex Using High-Throughput Array and Sequencing Platforms. |
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| المؤلفون: | Love RR; Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556.; Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556., Pombi M; Dipartimento di Sanità Pubblica e Malattie Infettive, Istituto Pasteur Italia-Fondazione Cenci-Bolognetti, Università di Roma 'La Sapienza', 00185 Rome, Italy., Guelbeogo MW; Centre National de Recherche et Formation sur le Paludisme (CNRFP) Ouagadougou, Burkina Faso., Campbell NR; GTseek LLC, Twin Falls, ID 83301., Stephens MT; Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556., Dabire RK; Institut de Recherche en Sciences de la Santé (IRSS)/Centre Muraz, Bobo-Dioulasso, Burkina Faso., Costantini C; Institut de Recherche en Sciences de la Santé (IRSS)/Centre Muraz, Bobo-Dioulasso, Burkina Faso.; Institut de Recherche pour le Développement (IRD) UMR MIVEGEC (University of Montpellier, CNRS 5290 IRD 224) Centre IRD de Montpellier, France., Della Torre A; Dipartimento di Sanità Pubblica e Malattie Infettive, Istituto Pasteur Italia-Fondazione Cenci-Bolognetti, Università di Roma 'La Sapienza', 00185 Rome, Italy., Besansky NJ; Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN 46556 nbesansk@nd.edu.; Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556. |
| المصدر: | G3 (Bethesda, Md.) [G3 (Bethesda)] 2020 Sep 02; Vol. 10 (9), pp. 3299-3307. Date of Electronic Publication: 2020 Sep 02. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101566598 Publication Model: Electronic Cited Medium: Internet ISSN: 2160-1836 (Electronic) Linking ISSN: 21601836 NLM ISO Abbreviation: G3 (Bethesda) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [Oxford] : Oxford University Press Original Publication: Bethesda, MD : Genetics Society of America, 2011- |
| مواضيع طبية MeSH: | Anopheles*/genetics , Malaria*, Africa, Western ; Animals ; Chromosome Inversion ; Genotype ; Mosquito Vectors |
| مستخلص: | Chromosomal inversion polymorphisms have special importance in the Anopheles gambiae complex of malaria vector mosquitoes, due to their role in local adaptation and range expansion. The study of inversions in natural populations is reliant on polytene chromosome analysis by expert cytogeneticists, a process that is limited by the rarity of trained specialists, low throughput, and restrictive sampling requirements. To overcome this barrier, we ascertained tag single nucleotide polymorphisms (SNPs) that are highly correlated with inversion status (inverted or standard orientation). We compared the performance of the tag SNPs using two alternative high throughput molecular genotyping approaches vs. traditional cytogenetic karyotyping of the same 960 individual An. gambiae and An. coluzzii mosquitoes sampled from Burkina Faso, West Africa. We show that both molecular approaches yield comparable results, and that either one performs as well or better than cytogenetics in terms of genotyping accuracy. Given the ability of molecular genotyping approaches to be conducted at scale and at relatively low cost without restriction on mosquito sex or developmental stage, molecular genotyping via tag SNPs has the potential to revitalize research into the role of chromosomal inversions in the behavior and ongoing adaptation of An. gambiae and An. coluzzii to environmental heterogeneities. (Copyright © 2020 Love et al.) |
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| معلومات مُعتمدة: | R01 AI125360 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: Anopheles gambiae; chromosomal inversion polymorphism; inversion genotyping; karyotyping; malaria vector; polytene chromosome analysis; tag SNP |
| تواريخ الأحداث: | Date Created: 20200719 Date Completed: 20210621 Latest Revision: 20210621 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC7467005 |
| DOI: | 10.1534/g3.120.401418 |
| PMID: | 32680855 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2160-1836 |
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| DOI: | 10.1534/g3.120.401418 |