دورية أكاديمية
Optimization, Purification and Antitumor Activity of Kodamaea ohmeri ANOMY L-Asparaginase Isolated from Banana Peel.
| العنوان: | Optimization, Purification and Antitumor Activity of Kodamaea ohmeri ANOMY L-Asparaginase Isolated from Banana Peel. |
|---|---|
| المؤلفون: | Shabana AMI; Microbiology Department-Faculty of Science Ain Shams University, Cairo, Egypt., Shetaia YM; Microbiology Department-Faculty of Science Ain Shams University, Cairo, Egypt., Abdelwahed NAM; Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Division, National Research Centre, 33 El Buhouth St. (Former El Tahrir St.), 12622, Dokki, Cairo, Egypt., Esawy MA; Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Division, National Research Centre, 33 El Buhouth St. (Former El Tahrir St.), 12622, Dokki, Cairo, Egypt., Alfarouk OR; Microbiology Department-Faculty of Science Ain Shams University, Cairo, Egypt. |
| المصدر: | Current pharmaceutical biotechnology [Curr Pharm Biotechnol] 2021; Vol. 22 (5), pp. 654-671. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bentham Science Publishers Country of Publication: Netherlands NLM ID: 100960530 Publication Model: Print Cited Medium: Internet ISSN: 1873-4316 (Electronic) Linking ISSN: 13892010 NLM ISO Abbreviation: Curr Pharm Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Hilversum, Netherlands ; Boca Raton, FL : Bentham Science Publishers, c2000- |
| مواضيع طبية MeSH: | Antineoplastic Agents/*pharmacology , Asparaginase/*pharmacology , Musa/*microbiology , Saccharomycetales/*chemistry, Antineoplastic Agents/chemistry ; Asparaginase/chemistry ; Asparaginase/isolation & purification ; Cell Line, Tumor ; Chromatography, Gel ; Drug Screening Assays, Antitumor ; Fermentation ; Humans |
| مستخلص: | Objective: L-Asparaginase is an important enzyme that converts L-asparagine to L-aspartate and ammonia. Microbial L-asparaginase has important applications as anticancer and food processing agents. Methods: This study reported the isolation, screening of a local yeast isolate from banana peel for L-asparaginase production using submerged fermentation, optimization of the production, purification, and anticancer assay of L-asparaginase. The yeast isolate was identified as Kodamaea ohmeri ANOMY based on the analysis of nuclear large subunit (26S) rDNA partial sequences. It was a promising L-asparaginase producer with a specific activity of 3059±193 U/mg in a non-optimized medium. The classical one-variable-at-a-time method was used to optimize the production medium components, and it was found that the elimination of K2HPO4 from the medium increased L-asparaginase specific activity (3100.90±180 U/mg). Results: Statistical optimization of L-asparaginase production was done using Plackett-Burman and Box-Behnken designs. The production medium for the maximum L-asparaginase specific activity (8500±578U/mg) was as follows (g/L): L-asparagine (7.50), NaNO3 (0.50), MgSO Conclusion: The cell morphology technique was used to evaluate the anticancer activity of L-asparaginase against three different cell lines. L-Asparaginase inhibited the growth of HepG-2, MCF-7, and HCT-116 cells at a concentration of 20, 50, and 60 μL, respectively. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.) |
| فهرسة مساهمة: | Keywords: L-Asparaginase; anticancer activity.; banana peel; purification; statistical optimization; yeast |
| المشرفين على المادة: | 0 (Antineoplastic Agents) EC 3.5.1.1 (Asparaginase) |
| SCR Organism: | Kodamaea ohmeri |
| تواريخ الأحداث: | Date Created: 20200725 Date Completed: 20210616 Latest Revision: 20210616 |
| رمز التحديث: | 20231215 |
| DOI: | 10.2174/1389201021666200723122300 |
| PMID: | 32707027 |
| قاعدة البيانات: | MEDLINE |
كن أول من يترك تعليقا!