دورية أكاديمية
Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase.
| العنوان: | Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase. |
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| المؤلفون: | Djeghader A; Department of Chemical Sciences and Bernal Institute, University of Limerick, Ireland. Tewfik.soulimane@ul.ie., Rossotti M; Aix Marseille Univ, CNRS, BIP UMR7281, Marseille, France. schoepp@imm.cnrs.fr stephane.grimaldi@univ-amu.fr., Abdulkarim S; Department of Chemical Sciences and Bernal Institute, University of Limerick, Ireland. Tewfik.soulimane@ul.ie., Biaso F; Aix Marseille Univ, CNRS, BIP UMR7281, Marseille, France. schoepp@imm.cnrs.fr stephane.grimaldi@univ-amu.fr., Gerbaud G; Aix Marseille Univ, CNRS, BIP UMR7281, Marseille, France. schoepp@imm.cnrs.fr stephane.grimaldi@univ-amu.fr., Nitschke W; Aix Marseille Univ, CNRS, BIP UMR7281, Marseille, France. schoepp@imm.cnrs.fr stephane.grimaldi@univ-amu.fr., Schoepp-Cothenet B; Aix Marseille Univ, CNRS, BIP UMR7281, Marseille, France. schoepp@imm.cnrs.fr stephane.grimaldi@univ-amu.fr., Soulimane T; Department of Chemical Sciences and Bernal Institute, University of Limerick, Ireland. Tewfik.soulimane@ul.ie., Grimaldi S; Aix Marseille Univ, CNRS, BIP UMR7281, Marseille, France. schoepp@imm.cnrs.fr stephane.grimaldi@univ-amu.fr. |
| المصدر: | Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2020 Aug 25; Vol. 56 (68), pp. 9850-9853. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 9610838 Publication Model: Print Cited Medium: Internet ISSN: 1364-548X (Electronic) Linking ISSN: 13597345 NLM ISO Abbreviation: Chem Commun (Camb) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Molybdenum/*chemistry , Phosphates/*chemistry , Sulfite Dehydrogenase/*chemistry, Catalytic Domain ; Crystallography, X-Ray ; Density Functional Theory ; Electron Spin Resonance Spectroscopy ; Hydrogen Bonding ; Sulfite Dehydrogenase/metabolism ; Thermus/enzymology |
| مستخلص: | By combining X-ray crystallography, electron paramagnetic resonance techniques and density functional theory-based modelling, we provide evidence for a direct coordination of the product analogue, phosphate, to the molybdenum active site of a sulfite dehydrogenase. This interaction is mimicking the still experimentally uncharacterized reaction intermediate proposed to arise during the catalytic cycle of this class of enzymes. This work opens new perspectives for further deciphering the reaction mechanism of this nearly ubiquitous class of oxidoreductases. |
| المشرفين على المادة: | 0 (Phosphates) 81AH48963U (Molybdenum) EC 1.8.2.1 (Sulfite Dehydrogenase) |
| تواريخ الأحداث: | Date Created: 20200728 Date Completed: 20210416 Latest Revision: 20210416 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1039/d0cc03634j |
| PMID: | 32716419 |
| قاعدة البيانات: | MEDLINE |
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