دورية أكاديمية
Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters.
| العنوان: | Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters. |
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| المؤلفون: | Umkehrer C; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Holstein F; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Formenti L; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Jude J; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Froussios K; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Neumann T; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Cronin SM; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Haas L; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Lipp JJ; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.; Boehringer Ingelheim RCV GmbH & Co. KG, Vienna, Austria., Burkard TR; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Fellner M; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria., Wiesner T; Department of Dermatology, Medical University of Vienna, Vienna, Austria., Zuber J; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria.; Medical University of Vienna, Vienna BioCenter (VBC), Vienna, Austria., Obenauf AC; Research Institute of Molecular Pathology (IMP), Vienna BioCenter (VBC), Vienna, Austria. anna.obenauf@imp.ac.at. |
| المصدر: | Nature biotechnology [Nat Biotechnol] 2021 Feb; Vol. 39 (2), pp. 174-178. Date of Electronic Publication: 2020 Jul 27. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature America Publishing Country of Publication: United States NLM ID: 9604648 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1546-1696 (Electronic) Linking ISSN: 10870156 NLM ISO Abbreviation: Nat Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Nature America Publishing Original Publication: New York, NY : Nature Pub. Co., [1996- |
| مواضيع طبية MeSH: | Cell Separation* , Genes, Reporter*, CRISPR-Cas Systems/*genetics , Clone Cells/*metabolism, Animals ; Cell Line ; Female ; Humans ; Melanoma/pathology ; Mice, Inbred C57BL ; Mitogen-Activated Protein Kinase Kinases/antagonists & inhibitors ; Protein Kinase Inhibitors/pharmacology ; raf Kinases/antagonists & inhibitors ; Mice |
| مستخلص: | We developed a functional lineage tracing tool termed CaTCH (CRISPRa tracing of clones in heterogeneous cell populations). CaTCH combines precise clonal tracing of millions of cells with the ability to retrospectively isolate founding clones alive before and during selection, allowing functional experiments. Using CaTCH, we captured rare clones representing as little as 0.001% of a population and investigated the emergence of resistance to targeted melanoma therapy in vivo. |
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| المشرفين على المادة: | 0 (Protein Kinase Inhibitors) EC 2.7.11.1 (raf Kinases) EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases) |
| تواريخ الأحداث: | Date Created: 20200729 Date Completed: 20210309 Latest Revision: 20240226 |
| رمز التحديث: | 20240226 |
| DOI: | 10.1038/s41587-020-0614-0 |
| PMID: | 32719478 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1546-1696 |
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| DOI: | 10.1038/s41587-020-0614-0 |