دورية أكاديمية
أعرض في EDS

Five-year cryopreservation at -80 °C of edible and medicinal basidiomycetes by wheat grain technique.

التفاصيل البيبلوغرافية
العنوان: Five-year cryopreservation at -80 °C of edible and medicinal basidiomycetes by wheat grain technique.
المؤلفون: Zaghi Junior LL; Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Praça Mascarenhas de Moraes 4282, Umuarama, PR 87502-210, Brazil., Bertéli MBD; Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Praça Mascarenhas de Moraes 4282, Umuarama, PR 87502-210, Brazil., de Freitas JDS; Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Praça Mascarenhas de Moraes 4282, Umuarama, PR 87502-210, Brazil., de Oliveira Filho OBQ; Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Praça Mascarenhas de Moraes 4282, Umuarama, PR 87502-210, Brazil., Lopes AD; Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Praça Mascarenhas de Moraes 4282, Umuarama, PR 87502-210, Brazil., Ruiz SP; Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Praça Mascarenhas de Moraes 4282, Umuarama, PR 87502-210, Brazil., do Valle JS; Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Praça Mascarenhas de Moraes 4282, Umuarama, PR 87502-210, Brazil., Linde GA; Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Praça Mascarenhas de Moraes 4282, Umuarama, PR 87502-210, Brazil., Colauto NB; Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Praça Mascarenhas de Moraes 4282, Umuarama, PR 87502-210, Brazil. Electronic address: nbc@prof.unipar.br.
المصدر: Journal of microbiological methods [J Microbiol Methods] 2020 Sep; Vol. 176, pp. 106030. Date of Electronic Publication: 2020 Aug 15.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, The Netherlands : Elsevier Biomedical, c1983-
مواضيع طبية MeSH: Basidiomycota* , Mycelium*, Cryopreservation/*methods, Cryoprotective Agents/chemistry ; Endosperm ; Microbiological Techniques ; Triticum
مستخلص: This research has focused on basidiomycete cryopreservation at -80 °C and developed a cryopreservation method based on the use of hard or medium-hard endosperm wheat grains as a mycelial carrier for cryopreservation. The aim of this study was to evaluate the mycelial viability of edible and medicinal basidiomycetes, using 13 strains of Agaricus spp. and eight strains of non-Agaricus spp., cryopreserved at -80 °C on hard endosperm wheat grain, with or without cryoprotectant agent (4% glucose), for two and five years. Two groups of basidiomycetes, Agaricus genus and other non-Agaricus genera, were cryopreserved at -80 °C by wheat grain technique for two and five years. The cryopreservation technique with hard endosperm wheat grain without cryoprotectant (preservation substrate), settled previously for A. subrufescens is efficient to cryopreserve other basidiomycetes such as Lentinus crinitus, Pleurotus ostreatus, Pleurotus eryngii, Schizophyllum commune, and Lentinula edodes, besides A. subrufescens strains.
Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Agaricus; Basidiomycete; Cryoprotectant; Long-term cryopreservation; Mushroom
المشرفين على المادة: 0 (Cryoprotective Agents)
تواريخ الأحداث: Date Created: 20200818 Date Completed: 20210722 Latest Revision: 20210722
رمز التحديث: 20221213
DOI: 10.1016/j.mimet.2020.106030
PMID: 32805366
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-8359
DOI:10.1016/j.mimet.2020.106030