دورية أكاديمية
Role of insulin in growth hormone-stimulated 3T3 cell adipogenesis.
| العنوان: | Role of insulin in growth hormone-stimulated 3T3 cell adipogenesis. |
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| المؤلفون: | Guller S; Memorial Sloan-Kettering Cancer Center, New York, New York 10021., Corin RE, Mynarcik DC, London BM, Sonenberg M |
| المصدر: | Endocrinology [Endocrinology] 1988 May; Vol. 122 (5), pp. 2084-9. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: United States NLM ID: 0375040 Publication Model: Print Cited Medium: Print ISSN: 0013-7227 (Print) Linking ISSN: 00137227 NLM ISO Abbreviation: Endocrinology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2017- : New York : Oxford University Press Original Publication: Los Angeles, Calif. : Association for the Study of Internal Secretions, |
| مواضيع طبية MeSH: | Adipose Tissue/*drug effects , Cell Differentiation/*drug effects , Growth Hormone/*pharmacology , Insulin/*pharmacology, Adipose Tissue/cytology ; Animals ; Cells, Cultured ; Glycerolphosphate Dehydrogenase/metabolism ; Kinetics ; Mice |
| مستخلص: | The role of insulin during GH-stimulated adipogenesis of 3T3-F442A fibroblasts was investigated. Adipogenesis in defined medium (DM), as quantified by the level of glycerol-3-phosphate dehydrogenase activity, revealed that there existed a strict requirement for both insulin and GH during adipogenesis. The concentration of insulin required to elicit half-maximal adipogenesis was approximately 20 nM. Insulin-like growth factor I was less effective than insulin in promoting adipogenesis, indicating that insulin action during differentiation was most likely mediated through the insulin receptor. Cellular viability was not compromised by the absence of insulin, as judged by colony-forming efficiency or trypan blue exclusion. Deletion of insulin from DM supplemented with 1 nM recombinant human GH reduced glycerol-3-phosphate dehydrogenase activity to uninduced levels. Removal of other individual DM constituents did not have this effect. The growth factors fibroblast growth factor, platelet-derived growth factor, and bombesin did not substitute for insulin during GH-stimulated adipogenesis. The characteristic increase in cell number observed during serum-based differentiation, reflecting clonal expansion of young adipocytes, did not occur in DM supplemented with insulin, and insulin-like growth factor I were necessary for this event. These results suggest that insulin functions in concert with GH as a coinducer of the differentiating signals. |
| معلومات مُعتمدة: | 1-F32-AM-07839 United States AM NIADDK NIH HHS; 5-F32-DK-07631 United States DK NIDDK NIH HHS; CA-38627 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (Insulin) 9002-72-6 (Growth Hormone) EC 1.1.- (Glycerolphosphate Dehydrogenase) |
| تواريخ الأحداث: | Date Created: 19880501 Date Completed: 19880601 Latest Revision: 20111117 |
| رمز التحديث: | 20240627 |
| DOI: | 10.1210/endo-122-5-2084 |
| PMID: | 3282874 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0013-7227 |
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| DOI: | 10.1210/endo-122-5-2084 |