دورية أكاديمية
Establishment and identification of primary bovine omasal epithelial cells.
| العنوان: | Establishment and identification of primary bovine omasal epithelial cells. |
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| المؤلفون: | Hu Z; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, People's Republic of China., Zhan K; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, People's Republic of China., Yang T; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, People's Republic of China., Zhao G; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, People's Republic of China., Lin M; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, People's Republic of China. linmiao@yzu.edu.cn. |
| المصدر: | In vitro cellular & developmental biology. Animal [In Vitro Cell Dev Biol Anim] 2020 Aug; Vol. 56 (7), pp. 495-499. Date of Electronic Publication: 2020 Aug 24. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 9418515 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1543-706X (Electronic) Linking ISSN: 10712690 NLM ISO Abbreviation: In Vitro Cell Dev Biol Anim Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Berlin : Springer Original Publication: Columbia, MD : Tissue Culture Association, c1993- |
| مواضيع طبية MeSH: | Epithelial Cells/*cytology , Omasum/*cytology, Animals ; Cattle ; Cell Proliferation ; Cell Shape ; Cells, Cultured ; Cellular Senescence ; Epithelial Cells/metabolism ; Keratin-18/metabolism |
| مستخلص: | The establishment and culture of bovine omasal epithelial cells (BOECs) in vitro is a valuable tool for the study of the physiological function, nutrient absorption, and transport mechanisms of the omasum in dairy cows. This paper proposes a method for the culture of primary BOECs. Trypsin digestion was used to subculture the BOECs, which were passaged for 20 generations in vitro, and showed typical epithelial-like characteristics and a cobblestone morphology. The primary BOECs had a fast growth phase (between days 4 and 5) and were validated by their slight β-galactosidase and visible cytokerat in 18 expression. In addition, RT-PCR results demonstrated that the monocarboxylate transporter 1 (MCT1), Na+/H+exchanger 1 (NHE1), and Na+/H+ exchanger 3 (NHE3) were expressed in the isolated primary BOECs. In conclusion, this primary BOEC isolation and culture model is a promising method for the study of nutrient absorption and regulation, as well as the immune regulation of epithelial cell transport in vitro. |
| معلومات مُعتمدة: | No. 31972589 National Natural Science Foundation of China; SBK2019043455 Natural Science Foundation of Jiangsu Province; CARS-36 Agriculture Research System of China; 2017YFD0502104-3 Development Project of China |
| المشرفين على المادة: | 0 (Keratin-18) |
| تواريخ الأحداث: | Date Created: 20200826 Date Completed: 20210610 Latest Revision: 20210610 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1007/s11626-020-00483-0 |
| PMID: | 32839903 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1543-706X |
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| DOI: | 10.1007/s11626-020-00483-0 |