دورية أكاديمية
A plant-based transient expression system for the rapid production of highly immunogenic Hepatitis E virus-like particles.
| العنوان: | A plant-based transient expression system for the rapid production of highly immunogenic Hepatitis E virus-like particles. |
|---|---|
| المؤلفون: | Mardanova ES; Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russia., Takova KH; Department of Plant Physiology and Molecular Biology, University of Plovdiv, 4000, Plovdiv, Bulgaria., Toneva VT; Department of Plant Physiology and Molecular Biology, University of Plovdiv, 4000, Plovdiv, Bulgaria.; Institute of Molecular Biology and Biotechnologies, 4000, Plovdiv, Bulgaria., Zahmanova GG; Department of Plant Physiology and Molecular Biology, University of Plovdiv, 4000, Plovdiv, Bulgaria.; Center of Plant Systems Biology and Biotechnology, 4000, Plovdiv, Bulgaria., Tsybalova LM; Research Institute of Influenza, Russian Ministry of Health, St. Petersburg, 197376, Russia., Ravin NV; Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, 119071, Russia. nravin@biengi.ac.ru. |
| المصدر: | Biotechnology letters [Biotechnol Lett] 2020 Nov; Vol. 42 (11), pp. 2441-2446. Date of Electronic Publication: 2020 Sep 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-6776 (Electronic) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1999- : Dordrecht : Kluwer Academic Publishers Original Publication: [Kew, Eng., Science and Technology Letters] |
| مواضيع طبية MeSH: | Mutation*, Hepatitis E/*prevention & control , Hepatitis E virus/*immunology , Influenza Vaccines/*administration & dosage , Nicotiana/*growth & development , Viral Proteins/*genetics, Animals ; Female ; Hepatitis Antibodies/blood ; Hepatitis E/immunology ; Hepatitis E virus/metabolism ; Immunization ; Influenza Vaccines/immunology ; Influenza Vaccines/metabolism ; Injections, Intramuscular ; Mice ; Recombinant Proteins/immunology ; Recombinant Proteins/metabolism ; Nicotiana/genetics ; Viral Proteins/immunology |
| مستخلص: | Objective: Hepatitis E virus (HEV) infection is a major cause of acute hepatitis worldwide. The aim of the study is the development of plant expression system for the production of virus-like particles formed by HEV capsid and the characterization of their immunogenicity. Results: Open reading frame (ORF) 2 encodes the viral capsid protein and possesses candidate for vaccine production. In this study, we used truncated genotype 3 HEV ORF 2 consisting of aa residues 110 to 610. The recombinant protein was expressed in Nicotiana benthamiana plants using the self-replicating potato virus X-based vector pEff up to 10% of the soluble protein fraction. The yield of HEV 110-610 after purification was 150-200 µg per 1 g of green leaf biomass. The recombinant protein formed nanosized virus-like particles. The immunization of mice with plant-produced HEV 110-610 protein induced high levels of HEV-specific serum antibodies. Conclusions: HEV ORF 2 (110-610 aa) can be used as candidate for the development of a plant-produced vaccine against Hepatitis E. |
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| معلومات مُعتمدة: | 18-54-18005 Российский Фонд Фундаментальных Исследований (РФФИ); DNTC/Russia 02-6 Bulgarian National Science Fund; 739582 Horizon 2020 Framework Programme |
| فهرسة مساهمة: | Keywords: Hepatitis E virus; Plant biofactory; Potato virus X; Vaccine; Viral vector |
| المشرفين على المادة: | 0 (Hepatitis Antibodies) 0 (Influenza Vaccines) 0 (ORF2 protein, Hepatitis E virus) 0 (Recombinant Proteins) 0 (Viral Proteins) |
| تواريخ الأحداث: | Date Created: 20200903 Date Completed: 20210705 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s10529-020-02995-x |
| PMID: | 32875477 |
| قاعدة البيانات: | MEDLINE |
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