دورية أكاديمية
EMPAS: Electron Microscopy Screening for Endogenous Protein Architectures.
| العنوان: | EMPAS: Electron Microscopy Screening for Endogenous Protein Architectures. |
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| المؤلفون: | Kim G; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea., Jang S; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea., Lee E; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea., Song JJ; Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea. |
| المصدر: | Molecules and cells [Mol Cells] 2020 Sep 30; Vol. 43 (9), pp. 804-812. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Korean Society for Molecular and Cellular Biology Country of Publication: United States NLM ID: 9610936 Publication Model: Print Cited Medium: Internet ISSN: 0219-1032 (Electronic) Linking ISSN: 10168478 NLM ISO Abbreviation: Mol Cells Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2014->: Seoul : Korean Society for Molecular and Cellular Biology Original Publication: Seoul : Korean Society for Molecular Biology, |
| مواضيع طبية MeSH: | Microscopy, Electron/*methods , Proteins/*chemistry, Animals ; Humans ; Mice ; Protein Conformation |
| مستخلص: | In cells, proteins form macromolecular complexes to execute their own unique roles in biological processes. Conventional structural biology methods adopt a bottom-up approach starting from defined sets of proteins to investigate the structures and interactions of protein complexes. However, this approach does not reflect the diverse and complex landscape of endogenous molecular architectures. Here, we introduce a top-down approach called Electron Microscopy screening for endogenous Protein ArchitectureS (EMPAS) to investigate the diverse and complex landscape of endogenous macromolecular architectures in an unbiased manner. By applying EMPAS, we discovered a spiral architecture and identified it as AdhE. Furthermore, we performed screening to examine endogenous molecular architectures of human embryonic stem cells (hESCs), mouse brains, cyanobacteria and plant leaves, revealing their diverse repertoires of molecular architectures. This study suggests that EMPAS may serve as a tool to investigate the molecular architectures of endogenous macromolecular proteins. |
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| فهرسة مساهمة: | Keywords: microscopy; protein; screening; structure |
| المشرفين على المادة: | 0 (Proteins) |
| تواريخ الأحداث: | Date Created: 20200925 Date Completed: 20210624 Latest Revision: 20240204 |
| رمز التحديث: | 20240205 |
| مُعرف محوري في PubMed: | PMC7528680 |
| DOI: | 10.14348/molcells.2020.0163 |
| PMID: | 32975210 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0219-1032 |
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| DOI: | 10.14348/molcells.2020.0163 |