دورية أكاديمية
Swine acute diarrhea syndrome coronavirus replication in primary human cells reveals potential susceptibility to infection.
| العنوان: | Swine acute diarrhea syndrome coronavirus replication in primary human cells reveals potential susceptibility to infection. |
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| المؤلفون: | Edwards CE; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Yount BL; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Graham RL; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Leist SR; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Hou YJ; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Dinnon KH 3rd; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Sims AC; Chemical and Biological Signatures Division, Pacific Northwest National Laboratory, Richland, WA 99354., Swanstrom J; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Gully K; Department of Comparative Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Scobey TD; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Cooley MR; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Currie CG; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Randell SH; Marsico Lung Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599., Baric RS; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; rbaric@email.unc.edu.; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599.; Rapidly Emerging Antiviral Drug Discovery Initiative, University of North Carolina, Chapel Hill, NC 27599. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2020 Oct 27; Vol. 117 (43), pp. 26915-26925. Date of Electronic Publication: 2020 Oct 12. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Virus Replication*/drug effects, Alphacoronavirus/*physiology , Coronavirus Infections/*virology , Disease Susceptibility/*virology, Adenosine Monophosphate/analogs & derivatives ; Adenosine Monophosphate/pharmacology ; Alanine/analogs & derivatives ; Alanine/pharmacology ; Alphacoronavirus/genetics ; Alphacoronavirus/growth & development ; Animals ; Cells, Cultured ; Chlorocebus aethiops ; Coronavirus Infections/transmission ; Gene Expression ; Host Specificity ; Humans ; Luminescent Proteins/genetics ; Mice ; Vero Cells |
| مستخلص: | Zoonotic coronaviruses represent an ongoing threat, yet the myriads of circulating animal viruses complicate the identification of higher-risk isolates that threaten human health. Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a newly discovered, highly pathogenic virus that likely evolved from closely related HKU2 bat coronaviruses, circulating in Rhinolophus spp. bats in China and elsewhere. As coronaviruses cause severe economic losses in the pork industry and swine are key intermediate hosts of human disease outbreaks, we synthetically resurrected a recombinant virus (rSADS-CoV) as well as a derivative encoding tomato red fluorescent protein (tRFP) in place of ORF3. rSADS-CoV replicated efficiently in a variety of continuous animal and primate cell lines, including human liver and rectal carcinoma cell lines. Of concern, rSADS-CoV also replicated efficiently in several different primary human lung cell types, as well as primary human intestinal cells. rSADS-CoV did not use human coronavirus ACE-2, DPP4, or CD13 receptors for docking and entry. Contemporary human donor sera neutralized the group I human coronavirus NL63, but not rSADS-CoV, suggesting limited human group I coronavirus cross protective herd immunity. Importantly, remdesivir, a broad-spectrum nucleoside analog that is effective against other group 1 and 2 coronaviruses, efficiently blocked rSADS-CoV replication in vitro. rSADS-CoV demonstrated little, if any, replicative capacity in either immune-competent or immunodeficient mice, indicating a critical need for improved animal models. Efficient growth in primary human lung and intestinal cells implicate SADS-CoV as a potential higher-risk emerging coronavirus pathogen that could negatively impact the global economy and human health. Competing Interests: The authors declare no competing interest. (Copyright © 2020 the Author(s). Published by PNAS.) |
| التعليقات: | Comment in: Proc Natl Acad Sci U S A. 2021 Jan 19;118(3):. (PMID: 33414272) |
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| معلومات مُعتمدة: | R01 AI089728 United States AI NIAID NIH HHS; R01 AI110964 United States AI NIAID NIH HHS; AI151797 United States NH NIH HHS; R01 AI132178 United States AI NIAID NIH HHS; AI132178 United States NH NIH HHS; AI142759 United States NH NIH HHS; DK065988 United States NH NIH HHS; U19 AI142759 United States AI NIAID NIH HHS; U01 AI151797 United States AI NIAID NIH HHS; P30 DK065988 United States DK NIDDK NIH HHS; AI089728 United States NH NIH HHS |
| فهرسة مساهمة: | Keywords: One Health; SADS; coronavirus; emerging infectious disease |
| المشرفين على المادة: | 0 (Luminescent Proteins) 3QKI37EEHE (remdesivir) 415SHH325A (Adenosine Monophosphate) OF5P57N2ZX (Alanine) |
| SCR Organism: | Swine acute diarrhea syndrome coronavirus |
| تواريخ الأحداث: | Date Created: 20201013 Date Completed: 20201109 Latest Revision: 20240430 |
| رمز التحديث: | 20240430 |
| مُعرف محوري في PubMed: | PMC7604506 |
| DOI: | 10.1073/pnas.2001046117 |
| PMID: | 33046644 |
| قاعدة البيانات: | MEDLINE |
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