دورية أكاديمية
[Role of LINC00310 in the glycidyl methacrylate-induced malignant transformation of human bronchial epithelial cell].
| العنوان: | [Role of LINC00310 in the glycidyl methacrylate-induced malignant transformation of human bronchial epithelial cell]. |
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| المؤلفون: | Ma S; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Wang Q; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Xie G; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Guo H; Center for Disease Control and Prevention of Xinbei District, Changzhou, Changzhou 213022, China., Song J; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Wuhan B; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Xu J; National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China. |
| المصدر: | Wei sheng yan jiu = Journal of hygiene research [Wei Sheng Yan Jiu] 2020 Sep; Vol. 49 (5), pp. 711-723. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: |
| أسماء مطبوعة: | Publication: Beijing Shi : "Wei sheng yan jiu" bian ji bu Original Publication: Beijing Shi : "Wei sheng yan jiu" bian ji bu, |
| مواضيع طبية MeSH: | Epoxy Compounds*/toxicity , Methacrylates*/toxicity, Cell Transformation, Neoplastic/genetics ; Epithelial Cells ; Humans ; RNA, Long Noncoding |
| مستخلص: | Objective: To investigate the expression and biological significance of LINC00310 in the malignant transformation of human bronchial epithelial cells(16 HBE) induced by glycidyl methacrylate(GMA). Methods: The 16 HBE cells recovered successfully used 1 μg/mL dimethyl sulfoxide(DMSO) as the solvent control group, and the final concentration was 8 μg/mL GMA as the treatment group, and were subcultured after repeated exposure 3 times for 72 hours each time. The 10 th, 20 th and 30 th generation cells of the GMA treatment group and corresponding DMSO control group were collected. The LncRNA microarrays was used to analyze the expression changes of LINC00310 in different periods, and the target gene and function prediction was performed by NCBI and cBioPortal bioinformatics database, and real-time quantification PCR(qPCR) was used to detect the relative expression levels of LINC00310 and predicted target genes. Results: The result of the microarray showed that LINC00310 in the GMA-treated group was down-regulated by 2. 02-fold, up-regulated by 6. 17-fold, and up-regulated by 2. 03-fold in the pre-transformation, mid-term, and late, respectively. The result of qPCR confirmed that the expression of LINC00310 relative expression level of 10 th, 20 th and 30 th generation cells was consistent with the microarray result, which were down-regulated by 2. 76-fold, up-regulated by 2. 68-fold, and up-regulated by 3. 09-fold. Consistently, the relative expression of the target gene C-Myc was statistically significant in 20 th and 30 th generation cells. Conclusion: LINC00310 induced low expression in the early stage of malignant transformation of 16 HBE cells induced by GMA, and was highly expressed in the middle and late stages. It indicated that LINC00310 may play a cancer-promoting role in the process of cell malignant transformation through C-Myc. |
| فهرسة مساهمة: | Keywords: LINC00310; glycidyl methacrylate; human bronchial epithelial cells; long non-coding RNA; malignant transformation |
| المشرفين على المادة: | 0 (Epoxy Compounds) 0 (LINC00312 long non-coding RNA, human) 0 (Methacrylates) 0 (RNA, Long Noncoding) R8WN29J8VF (glycidyl methacrylate) |
| تواريخ الأحداث: | Date Created: 20201019 Date Completed: 20201020 Latest Revision: 20220531 |
| رمز التحديث: | 20221213 |
| DOI: | 10.19813/j.cnki.weishengyanjiu.2020.05.003 |
| PMID: | 33070810 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1000-8020 |
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| DOI: | 10.19813/j.cnki.weishengyanjiu.2020.05.003 |