دورية أكاديمية
أعرض في EDS

Novel antigenic proteins of Mycoplasma agalactiae as potential vaccine and serodiagnostic candidates.

التفاصيل البيبلوغرافية
العنوان: Novel antigenic proteins of Mycoplasma agalactiae as potential vaccine and serodiagnostic candidates.
المؤلفون: Barbosa MS; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, Brazil., Alves RPDS; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, Brazil., Rezende IS; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, Brazil., Pereira SS; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, Brazil., Campos GB; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, Brazil; Multidisciplinary Institute of Health, Universidade Federal da Bahia, St. Rio de Contas, 58, Vitória da Conquista, Brazil., Freitas LM; Multidisciplinary Institute of Health, Universidade Federal da Bahia, St. Rio de Contas, 58, Vitória da Conquista, Brazil., Chopra-Dewasthaly R; Institute of Microbiology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210, Vienna, Austria., Ferreira LCS; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, Brazil., Guimarães AMS; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, Brazil., Marques LM; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, Brazil; Multidisciplinary Institute of Health, Universidade Federal da Bahia, St. Rio de Contas, 58, Vitória da Conquista, Brazil. Electronic address: lucasm@ufba.br., Timenetsky J; Department of Microbiology, Institute of Biomedical Science, University of São Paulo, Av. Prof. Lineu Prestes, 1374, São Paulo, Brazil.
المصدر: Veterinary microbiology [Vet Microbiol] 2020 Dec; Vol. 251, pp. 108866. Date of Electronic Publication: 2020 Sep 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7705469 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2542 (Electronic) Linking ISSN: 03781135 NLM ISO Abbreviation: Vet Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier Scientific Pub. Co.
مواضيع طبية MeSH: Antibodies, Bacterial/*immunology , Antigens, Bacterial/*immunology , Bacterial Vaccines/*immunology , Mycoplasma agalactiae/*chemistry , Serologic Tests/*methods, Animals ; Antigens, Bacterial/genetics ; Epitopes, B-Lymphocyte/immunology ; Female ; Genome, Bacterial ; Membrane Proteins/genetics ; Membrane Proteins/immunology ; Mycoplasma agalactiae/genetics ; Mycoplasma agalactiae/immunology ; Rabbits
مستخلص: Contagious agalactia (CA) is a serious disease notifiable to the World Organisation for Animal Health (OIE) causing severe economic losses to sheep and goat producers worldwide. Mycoplasma agalactiae, considered as its main etiological agent, inflicts a variety of symptoms in infected animals, including keratoconjunctivitis, mastitis, arthritis, ankylosis, abortions, stillbirths and granular vulvovaginitis. Despite its significance, developing a successful vaccine remains elusive, mostly due to the lack of knowledge about M. agalactiae's pathogenicity factors and pathogenic mechanisms, including its "core" antigens. The aim of this study was to identify, characterize and express antigenic proteins of M. agalactiae as potential vaccine candidates. Predicted proteins of type strain PG2 were analyzed using bioinformatic algorithms to assess their cellular localization and to identify their linear and conformational epitopes for B cells. Out of a total of 156 predicted membrane proteins, three were shortlisted as potential antigenic surface proteins, namely [MAG_1560 (WP_011949336.1), MAG_6130 (WP_011949770.1) and P40 (WP_011949418.1)]. These proteins were expressed in recombinant Escherichia coli strains. Purified proteins were evaluated for their antigenicity using Western blot and ELISA using sera of M. agalactiae-naturally infected and non-infected sheep and goats. All 3 proteins were specifically recognized by the tested sera of M. agalactiae-infected animals. Also, specific rabbit antisera raised against each of these 3 proteins confirm their membrane localization using TritonX-114 phase partioning, Western and colony immunoblotting. In conclusion, our study successfully identified P40 (as proof of concept and validation) and two novel antigenic M. agalactiae proteins as potential candidates for developing effective CA vaccines.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Agalactia; Bacterial epitopes; Contagious; Mycoplasma agalactiae; Recombinant antigens; Small ruminants
المشرفين على المادة: 0 (Antibodies, Bacterial)
0 (Antigens, Bacterial)
0 (Bacterial Vaccines)
0 (Epitopes, B-Lymphocyte)
0 (Membrane Proteins)
تواريخ الأحداث: Date Created: 20201025 Date Completed: 20210715 Latest Revision: 20210715
رمز التحديث: 20231215
DOI: 10.1016/j.vetmic.2020.108866
PMID: 33099078
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2542
DOI:10.1016/j.vetmic.2020.108866