دورية أكاديمية
Hepatic LC3 II/I ratio is not modulated in exercised mice.
| العنوان: | Hepatic LC3 II/I ratio is not modulated in exercised mice. |
|---|---|
| المؤلفون: | Marafon BB; School of Physical Education and Sport of Ribeirao Preto, University of Sao Paulo (USP), Ribeirao Preto, Sao Paulo, Brazil. adelinosanchez@usp.br., Pinto AP, da Rocha AL, Rovina RL, Pauli JR, De Moura LP, Cintra DE, Ropelle ER, Da Silva ASR |
| المصدر: | Physiological research [Physiol Res] 2020 Dec 22; Vol. 69 (6), pp. 1103-1111. Date of Electronic Publication: 2020 Nov 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for the Institute of Physiology of the Czechoslovak Academy of Sciences by Academia, Pub. House of the Academy Country of Publication: Czech Republic NLM ID: 9112413 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1802-9973 (Electronic) Linking ISSN: 08628408 NLM ISO Abbreviation: Physiol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Praha : Published for the Institute of Physiology of the Czechoslovak Academy of Sciences by Academia, Pub. House of the Academy |
| مواضيع طبية MeSH: | Liver/*metabolism , Microtubule-Associated Proteins/*metabolism , Muscle, Skeletal/*metabolism , Physical Conditioning, Animal/*physiology, Animals ; Autophagy/physiology ; Male ; Mice ; Mice, Inbred C57BL ; Microtubule-Associated Proteins/genetics ; Physical Endurance ; Signal Transduction |
| مستخلص: | Autophagy plays an essential role in body homeostasis achievement. One of the main proteins involved in this process is the LC3I, which, after lipidation, leads to the formation of LC3II that participates in the formation and maturation of autophagosome. This descriptive study verified the responses of LC3II to LC3I proteins, as well as the time-course of this ratio in mice livers after different types of acute physical exercise protocols. Eight-week-old male C57BL/6 mice were maintained three per cage with controlled temperature (22±2 °C) on a 12:12-h light-dark normal cycle with food (Purina chow) and water ad libitum. Mice were randomly divided into four groups: control (CT, sedentary mice), resistance (RE, submitted to a single bout of resistance exercise), endurance (EE, submitted to a single bout of endurance exercise), and concurrent (CE, submitted to a single bout of endurance combined with resistance exercise). The mice livers were extracted and used for the immunoblotting technique. The hepatic LC3B II/I ratio for the RE and EE groups were not altered during the different time-points. For the CE group, there was a decrease in this ratio 12h after exercise compared to time 0 and 18h. Also, the hepatic LC3B II/I ratios were not different among the acute physical exercise protocols along the time-course. The hepatic LC3B II/I ratio was not influenced by the endurance and resistance protocols but decreased in response to the concurrent protocol at 12h after the stimulus. |
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| المشرفين على المادة: | 0 (Map1lc3b protein, mouse) 0 (Microtubule-Associated Proteins) |
| تواريخ الأحداث: | Date Created: 20201101 Date Completed: 20211014 Latest Revision: 20220210 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8549879 |
| DOI: | 10.33549/physiolres.934441 |
| PMID: | 33129244 |
| قاعدة البيانات: | MEDLINE |
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