دورية أكاديمية
Lipopolysaccharide triggers different transcriptional signatures in taurine and indicine cattle macrophages: Reactive oxygen species and potential outcomes to the development of immune response to infections.
| العنوان: | Lipopolysaccharide triggers different transcriptional signatures in taurine and indicine cattle macrophages: Reactive oxygen species and potential outcomes to the development of immune response to infections. |
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| المؤلفون: | Daibert RMP; Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil., de Biagi Junior CAO; Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Vieira FO; Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil., da Silva MVGB; Embrapa-Dairy Cattle Research Centre, Juiz de Fora, Minas Gerais, Brazil., Hottz ED; Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil., Mendonça Pinheiro MB; Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil., Faza DRLR; Embrapa-Dairy Cattle Research Centre, Juiz de Fora, Minas Gerais, Brazil., Pereira HP; Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil., Martins MF; Embrapa-Dairy Cattle Research Centre, Juiz de Fora, Minas Gerais, Brazil., Brandão HM; Embrapa-Dairy Cattle Research Centre, Juiz de Fora, Minas Gerais, Brazil., Machado MA; Embrapa-Dairy Cattle Research Centre, Juiz de Fora, Minas Gerais, Brazil., Carvalho WA; Embrapa-Dairy Cattle Research Centre, Juiz de Fora, Minas Gerais, Brazil. |
| المصدر: | PloS one [PLoS One] 2020 Nov 06; Vol. 15 (11), pp. e0241861. Date of Electronic Publication: 2020 Nov 06 (Print Publication: 2020). |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
| مواضيع طبية MeSH: | Breeding* , Cattle*, Gene Expression Profiling/*veterinary , Gene Regulatory Networks/*drug effects , Lipopolysaccharides/*pharmacology , Macrophages/*chemistry, Animals ; Cell Differentiation/drug effects ; Gene Expression Regulation/drug effects ; Lipopolysaccharides/adverse effects ; Macrophage Activation ; Macrophages/drug effects ; Reactive Oxygen Species/metabolism ; Sequence Analysis, RNA/veterinary ; Species Specificity |
| مستخلص: | Macrophages are classified upon activation as classical activated M1 and M2 anti-inflammatory regulatory populations. This macrophage polarization is well characterized in humans and mice, but M1/M2 profile in cattle has been far less explored. Bos primigenius taurus (taurine) and Bos primigenius indicus (indicine) cattle display contrasting levels of resistance to infection and parasitic diseases such as C57BL/6J and Balb/c murine experimental models of parasite infection outcomes based on genetic background. Thus, we investigated the differential gene expression profile of unstimulated and LPS stimulated monocyte-derived macrophages (MDMs) from Holstein (taurine) and Gir (indicine) breeds using RNA sequencing methodology. For unstimulated MDMs, the contrast between Holstein and Gir breeds identified 163 Differentially Expressed Genes (DEGs) highlighting the higher expression of C-C chemokine receptor type five (CCR5) and BOLA-DQ genes in Gir animals. LPS-stimulated MDMs from Gir and Holstein animals displayed 1,257 DEGs enriched for cell adhesion and inflammatory responses. Gir MDMs cells displayed a higher expression of M1 related genes like Nitric Oxide Synthase 2 (NOS2), Toll like receptor 4 (TLR4), Nuclear factor NF-kappa-B 2 (NFKB2) in addition to higher levels of transcripts for proinflammatory cytokines, chemokines, complement factors and the acute phase protein Serum Amyloid A (SAA). We also showed that gene expression of inflammatory M1 population markers, complement and SAA genes was higher in Gir in buffy coat peripheral cells in addition to nitric oxide concentration in MDMs supernatant and animal serum. Co-expression analyses revealed that Holstein and Gir animals showed different transcriptional signatures in the MDMs response to LPS that impact on cell cycle regulation, leukocyte migration and extracellular matrix organization biological processes. Overall, the results suggest that Gir animals show a natural propensity to generate a more pronounced M1 inflammatory response than Holstein, which might account for a faster immune response favouring resistance to many infection diseases. Competing Interests: The authors have declared that no competing interests exist. |
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| المشرفين على المادة: | 0 (Lipopolysaccharides) 0 (Reactive Oxygen Species) |
| تواريخ الأحداث: | Date Created: 20201106 Date Completed: 20210111 Latest Revision: 20240803 |
| رمز التحديث: | 20240803 |
| مُعرف محوري في PubMed: | PMC7647108 |
| DOI: | 10.1371/journal.pone.0241861 |
| PMID: | 33156842 |
| قاعدة البيانات: | MEDLINE |
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