دورية أكاديمية
Innate immune activation by conditioned medium of cancer cells following combined phototherapy with photosensitizer-loaded gold nanorods.
| العنوان: | Innate immune activation by conditioned medium of cancer cells following combined phototherapy with photosensitizer-loaded gold nanorods. |
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| المؤلفون: | Kang MWC; NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, University Hall, Tan Chin Tuan Wing, Level 04, #04-02, 21 Lower Kent Ridge, Singapore 119077, Singapore. biekahj@nus.edu.sg., Liu H, Kah JCY |
| المصدر: | Journal of materials chemistry. B [J Mater Chem B] 2020 Dec 21; Vol. 8 (47), pp. 10812-10824. Date of Electronic Publication: 2020 Nov 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101598493 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2050-7518 (Electronic) Linking ISSN: 2050750X NLM ISO Abbreviation: J Mater Chem B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : Royal Society of Chemistry |
| مواضيع طبية MeSH: | Nanotubes*/chemistry, Gold/*administration & dosage , Immunity, Innate/*drug effects , Metal Nanoparticles/*administration & dosage , Photosensitizing Agents/*administration & dosage , Phototherapy/*methods, Animals ; Cell Line, Tumor ; Cell Survival/drug effects ; Cell Survival/physiology ; Combined Modality Therapy/methods ; Culture Media, Conditioned/chemistry ; Culture Media, Conditioned/pharmacology ; Gold/chemistry ; Immunity, Innate/physiology ; Metal Nanoparticles/chemistry ; Mice ; Mice, Nude ; Photochemotherapy/methods ; Photosensitizing Agents/chemical synthesis ; Xenograft Model Antitumor Assays/methods |
| مستخلص: | Nanoparticle-based phototherapy has evolved to include immunotherapy as an effective treatment combination for cancers through inducing anti-cancer immune activation leading to downstream adaptive responses and immune protection. However, most cancer phototherapy studies that claimed anti-cancer immunogenic effects often included exogenous immunostimulants to potentiate immune responses and did not clearly establish their effects on immune cells. In this study, we showed that combined photodynamic (PDT) and photothermal therapy (PTT) using gold nanorods (NRs) loaded with the photosensitizer chlorin e6 (Ce6) on endogenously formed mouse serum (MS) protein coronas (i.e., NR-MS-Ce6) on EMT6 murine mammary carcinoma cells could potentiate the activation of both J774A.1 macrophages and DC2.4 dendritic cells. The activation of these innate immune cells by the conditioned media from cancer cells treated with combined PDT + PTT was cell-type and number dependent. While treated B16-OVA murine melanoma cells induced lower activation levels for both immune cell types compared to EMT6, they caused higher pro-inflammatory cytokine secretion levels. Our study suggests the importance of immunological investigations to complement any nanoparticle-based therapeutic interventions to better evaluate their efficacy. This could be achieved through a simple approach to screen for the first line of immune responses arising from these therapies prior to in vivo studies. |
| المشرفين على المادة: | 0 (Culture Media, Conditioned) 0 (Photosensitizing Agents) 7440-57-5 (Gold) |
| تواريخ الأحداث: | Date Created: 20201111 Date Completed: 20210324 Latest Revision: 20210324 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1039/d0tb01953d |
| PMID: | 33174587 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2050-7518 |
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| DOI: | 10.1039/d0tb01953d |