دورية أكاديمية
Allelic diversity and forensic estimations of the Beijing Hans: Comparative data on sequence-based and length-based STRs.
| العنوان: | Allelic diversity and forensic estimations of the Beijing Hans: Comparative data on sequence-based and length-based STRs. |
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| المؤلفون: | Chen QF; National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China., Kang KL; National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China., Song JJ; National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China., Zhang C; National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China., Yu ZL; National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China., Zhao GB; National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China., Wu H; National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China., Ji AQ; National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China. Electronic address: aqjdna@163.com., Ye J; National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China. Electronic address: yejian77@126.com., Wang L; National Engineering Laboratory for Forensic Science, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China; Key Laboratory of Forensic Genetics of Ministry of Public Security, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China. Electronic address: wangle_02@163.com. |
| المصدر: | Forensic science international. Genetics [Forensic Sci Int Genet] 2021 Mar; Vol. 51, pp. 102424. Date of Electronic Publication: 2020 Nov 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101317016 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-0326 (Electronic) Linking ISSN: 18724973 NLM ISO Abbreviation: Forensic Sci Int Genet Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Alleles* , Genetic Variation* , Microsatellite Repeats*, Ethnicity/*genetics, China ; DNA Fingerprinting ; Genetics, Population ; High-Throughput Nucleotide Sequencing ; Humans ; Polymerase Chain Reaction ; Sequence Analysis, DNA |
| مستخلص: | Short tandem repeat (STR) profiling is routinely used in forensic genetics. At present, STR analysis is mainly performed by capillary electrophoresis (CE). However, due to limitations associated with the CE method, STR genotyping has been limited to length polymorphisms only. Because next generation sequencing (NGS) is capable of providing full resolution STR data at the sequence variation level, the individual identification capability of forensic STR loci could be significantly improved. Here we present sequence-based STR data for the Beijing Han population in which 291 individuals were screened for 23 commonly used forensic STRs using the SeqTypeR24 CASE kit on an Ion PGM platform. In total, 234 length-based alleles and 356 sequence-based alleles, which included 22 novel core repeat sequences, were observed. The sequence-based matching probability and power of discrimination were superior to the length-based numbers for 16 loci bearing micro-variant alleles. Combined matching probability reached 8.2 × 10 -29 for 23 STR loci at the sequence level. This was two orders of magnitude higher than the parameters at length level and provides a data base for sequence-based STR casework applications. (Copyright © 2020 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Forensic genetics; Next generation sequencing; Sequence polymorphism; Short tandem repeat |
| تواريخ الأحداث: | Date Created: 20201128 Date Completed: 20210729 Latest Revision: 20211204 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.fsigen.2020.102424 |
| PMID: | 33248347 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1878-0326 |
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| DOI: | 10.1016/j.fsigen.2020.102424 |