دورية أكاديمية
أعرض في EDS

In silico APC/C substrate discovery reveals cell cycle-dependent degradation of UHRF1 and other chromatin regulators.

التفاصيل البيبلوغرافية
العنوان: In silico APC/C substrate discovery reveals cell cycle-dependent degradation of UHRF1 and other chromatin regulators.
المؤلفون: Franks JL; Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Martinez-Chacin RC; Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Wang X; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Tiedemann RL; Center for Epigenetics, Van Andel Research Institute, Grand Rapids, Michigan, United States of America., Bonacci T; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Choudhury R; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Bolhuis DL; Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Enrico TP; Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Mouery RD; Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Department of Genetics, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Damrauer JS; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Yan F; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Harrison JS; Department of Chemistry, University of the Pacific, Stockton, California, United States of America., Major MB; Department of Cell Biology and Physiology, Department of Otolaryngology, Washington University in St. Louis, St. Louis, Missouri, United States of America., Hoadley KA; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Department of Genetics, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Suzuki A; McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin, Madison, Wisconsin, United States of America., Rothbart SB; Center for Epigenetics, Van Andel Research Institute, Grand Rapids, Michigan, United States of America., Brown NG; Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America., Emanuele MJ; Department of Pharmacology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.; Lineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, United States of America.
المصدر: PLoS biology [PLoS Biol] 2020 Dec 11; Vol. 18 (12), pp. e3000975. Date of Electronic Publication: 2020 Dec 11 (Print Publication: 2020).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101183755 Publication Model: eCollection Cited Medium: Internet ISSN: 1545-7885 (Electronic) Linking ISSN: 15449173 NLM ISO Abbreviation: PLoS Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science, [2003]-
مواضيع طبية MeSH: Anaphase-Promoting Complex-Cyclosome/*metabolism , CCAAT-Enhancer-Binding Proteins/*metabolism , Chromatin/*metabolism , Ubiquitin-Protein Ligases/*metabolism, Anaphase-Promoting Complex-Cyclosome/physiology ; CCAAT-Enhancer-Binding Proteins/genetics ; CCAAT-Enhancer-Binding Proteins/physiology ; Cell Cycle/physiology ; Cell Cycle Proteins/metabolism ; Cell Line ; Chromatin/genetics ; Computer Simulation ; HEK293 Cells ; HeLa Cells ; Humans ; Protein Processing, Post-Translational ; Transcription Factors/metabolism ; Ubiquitin-Protein Ligases/physiology ; Ubiquitination
مستخلص: The anaphase-promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase and critical regulator of cell cycle progression. Despite its vital role, it has remained challenging to globally map APC/C substrates. By combining orthogonal features of known substrates, we predicted APC/C substrates in silico. This analysis identified many known substrates and suggested numerous candidates. Unexpectedly, chromatin regulatory proteins are enriched among putative substrates, and we show experimentally that several chromatin proteins bind APC/C, oscillate during the cell cycle, and are degraded following APC/C activation, consistent with being direct APC/C substrates. Additional analysis revealed detailed mechanisms of ubiquitylation for UHRF1, a key chromatin regulator involved in histone ubiquitylation and DNA methylation maintenance. Disrupting UHRF1 degradation at mitotic exit accelerates G1-phase cell cycle progression and perturbs global DNA methylation patterning in the genome. We conclude that APC/C coordinates crosstalk between cell cycle and chromatin regulatory proteins. This has potential consequences in normal cell physiology, where the chromatin environment changes depending on proliferative state, as well as in disease.
Competing Interests: I have read the journal's policy and the authors of this manuscript have the following competing interests: NGB is a consultant for Cullgen, Inc.
References: Methods Mol Biol. 2009;545:301-12. (PMID: 19475397)
Cell. 2007 Jul 13;130(1):127-39. (PMID: 17632060)
Nat Struct Mol Biol. 2012 Nov;19(11):1155-60. (PMID: 23022729)
Science. 2007 Sep 21;317(5845):1760-4. (PMID: 17673620)
Nature. 1999 Oct 21;401(6755):815-8. (PMID: 10548110)
Nat Cell Biol. 2009 Nov;11(11):1363-9. (PMID: 19820702)
Stem Cells. 2018 Nov;36(11):1736-1751. (PMID: 29999568)
Epigenetics. 2009 Jan;4(1):8-14. (PMID: 19077538)
Cell Cycle. 2007 Oct 15;6(20):2516-23. (PMID: 17726374)
Mol Cell. 2015 Sep 3;59(5):867-81. (PMID: 26051181)
Cancer Cell. 2019 Apr 15;35(4):633-648.e7. (PMID: 30956060)
Nature. 2007 Dec 6;450(7171):908-12. (PMID: 17994007)
J Cell Biol. 2010 Jun 14;189(6):981-96. (PMID: 20548101)
Nature. 2017 Sep 13;549(7671):219-226. (PMID: 28905911)
Sci Signal. 2010 Jan 12;3(104):ra3. (PMID: 20068231)
Cancer Discov. 2017 Apr;7(4):424-441. (PMID: 28174173)
Epigenomics. 2009 Oct;1(1):177-200. (PMID: 22122642)
Cell. 2011 Mar 4;144(5):646-74. (PMID: 21376230)
Nature. 2014 Sep 18;513(7518):388-393. (PMID: 25043029)
Cancer Cell. 2010 Oct 19;18(4):382-95. (PMID: 20951947)
Cancer Res. 2011 May 15;71(10):3447-52. (PMID: 21270108)
Genes (Basel). 2018 Dec 03;9(12):. (PMID: 30513966)
Nature. 1996 Jul 25;382(6589):319-24. (PMID: 8684459)
Biochemistry. 1993 Sep 21;32(37):9859-65. (PMID: 8373784)
Methods Enzymol. 2005;399:21-36. (PMID: 16338346)
Nat Commun. 2015 Jan 06;6:5906. (PMID: 25562820)
Cancer Cell. 2014 Feb 10;25(2):196-209. (PMID: 24486181)
Mol Cell Biol. 2008 Sep;28(17):5162-71. (PMID: 18573889)
Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2543-8. (PMID: 19181856)
Nat Rev Mol Cell Biol. 2015 Feb;16(2):82-94. (PMID: 25604195)
Nucleic Acids Res. 2018 Nov 16;46(20):e123. (PMID: 30085201)
Nucleic Acids Res. 2015 Apr 20;43(7):e47. (PMID: 25605792)
J Cell Sci. 2010 Feb 1;123(Pt 3):321-30. (PMID: 20053638)
Biochim Biophys Acta Mol Cell Res. 2018 Dec;1865(12):1924-1933. (PMID: 30290241)
EMBO J. 2014 Dec 1;33(23):2860-79. (PMID: 25349192)
Genes Dev. 2013 Jun 1;27(11):1288-98. (PMID: 23752590)
Elife. 2016 Sep 06;5:. (PMID: 27595565)
Mol Biol Cell. 2013 Oct;24(19):3025-37. (PMID: 23924899)
Cell. 2015 Jul 16;162(2):425-440. (PMID: 26186194)
Mol Biol Cell. 2013 Dec;24(23):3634-50. (PMID: 24109597)
PLoS Biol. 2019 Sep 3;17(9):e3000378. (PMID: 31479438)
Genes Dev. 2002 Feb 15;16(4):503-17. (PMID: 11850412)
Am J Hum Genet. 2019 Sep 5;105(3):625-630. (PMID: 31303264)
Nat Cell Biol. 2008 Jul;10(7):802-11. (PMID: 18552834)
Cell Discov. 2017 Jun 13;3:17019. (PMID: 28626588)
Nature. 2006 Apr 13;440(7086):954-8. (PMID: 16612388)
Mol Cell. 2011 Oct 21;44(2):325-40. (PMID: 21906983)
BMC Med Genomics. 2011 Jan 09;4:3. (PMID: 21214954)
Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2570-8. (PMID: 27114510)
Nature. 2013 Oct 10;502(7470):249-53. (PMID: 24013172)
Cell. 2005 Sep 23;122(6):915-26. (PMID: 16153703)
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. (PMID: 21890473)
Cell Rep. 2013 Apr 25;3(4):1105-16. (PMID: 23545495)
Nature. 2009 Nov 19;462(7271):315-22. (PMID: 19829295)
Cell Rep. 2017 Sep 26;20(13):3212-3222. (PMID: 28954236)
Nucleic Acids Res. 2013 Mar 1;41(5):2846-56. (PMID: 23325852)
Cell Cycle. 2013 Sep 15;12(18):2992-3000. (PMID: 23974109)
Cell Rep. 2015 Mar 31;10(12):1957-66. (PMID: 25818288)
Genome Biol. 2004;5(10):R80. (PMID: 15461798)
Genomics. 2011 Oct;98(4):288-95. (PMID: 21839163)
Cell. 2000 Aug 18;102(4):411-24. (PMID: 10966104)
Mol Biol Cell. 2011 Jul 1;22(13):2175-84. (PMID: 21562221)
Immunity. 2018 Apr 17;48(4):812-830.e14. (PMID: 29628290)
Mol Cell Proteomics. 2014 Sep;13(9):2411-25. (PMID: 24857844)
Nat Struct Mol Biol. 2013 Jul;20(7):827-35. (PMID: 23708605)
Mol Biol Cell. 2002 Jun;13(6):1977-2000. (PMID: 12058064)
EMBO J. 2001 Feb 15;20(4):792-801. (PMID: 11179223)
EMBO J. 2018 Aug 15;37(16):. (PMID: 29973362)
Open Biol. 2017 Nov;7(11):. (PMID: 29167309)
Mol Cell. 2010 May 28;38(4):576-89. (PMID: 20513432)
Proc Natl Acad Sci U S A. 2015 Apr 28;112(17):5272-9. (PMID: 25825779)
Mol Cell. 2016 Oct 6;64(1):12-23. (PMID: 27716480)
Nucleus. 2015;6(2):123-32. (PMID: 25891992)
Cell. 2016 Jun 30;166(1):167-80. (PMID: 27368103)
Nat Commun. 2016 Jan 05;7:10201. (PMID: 26727879)
Mol Cell. 2013 Jun 6;50(5):649-60. (PMID: 23707760)
Nat Commun. 2018 Nov 14;9(1):4776. (PMID: 30429481)
Cell Syst. 2016 Oct 26;3(4):395-403.e4. (PMID: 27667366)
Proc Natl Acad Sci U S A. 2009 Oct 27;106(43):18213-8. (PMID: 19822757)
J Neurochem. 2019 Oct;151(1):103-115. (PMID: 31318984)
F1000Res. 2016 Jun 08;5:1281. (PMID: 27347385)
Annu Rev Cell Dev Biol. 2018 Oct 6;34:137-162. (PMID: 30110556)
Cell. 2018 Apr 5;173(2):291-304.e6. (PMID: 29625048)
Genome Res. 2011 Nov;21(11):1833-40. (PMID: 21957152)
Methods Enzymol. 2005;399:404-14. (PMID: 16338372)
Nat Methods. 2015 Feb;12(2):115-21. (PMID: 25633503)
Proc Natl Acad Sci U S A. 2008 Jan 22;105(3):955-60. (PMID: 18195366)
Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. (PMID: 18669648)
Cancer Discov. 2017 Feb;7(2):218-233. (PMID: 28069571)
Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13415-20. (PMID: 18757745)
Mol Biol Cell. 2020 Apr 1;31(8):725-740. (PMID: 31995441)
Oncotarget. 2017 Apr 24;8(31):51946-51962. (PMID: 28881702)
Bioinformatics. 2010 Mar 15;26(6):841-2. (PMID: 20110278)
Cell. 2013 Feb 28;152(5):1160-72. (PMID: 23452859)
Cell. 2011 Oct 14;147(2):459-74. (PMID: 21963094)
Cell. 2014 May 8;157(4):910-21. (PMID: 24813613)
Front Genet. 2015 Feb 03;6:19. (PMID: 25691891)
EMBO J. 2014 Feb 18;33(4):385-99. (PMID: 24510915)
Cell. 2016 Jun 2;165(6):1440-1453. (PMID: 27259151)
Cell Res. 2015 Aug;25(8):911-29. (PMID: 26065575)
معلومات مُعتمدة: P30 CA016086 United States CA NCI NIH HHS; T32 GM007040 United States GM NIGMS NIH HHS; R01 GM120309 United States GM NIGMS NIH HHS; T32 CA071341 United States CA NCI NIH HHS; R35 GM128855 United States GM NIGMS NIH HHS; R35 GM124736 United States GM NIGMS NIH HHS; R01 GM134231 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (CCAAT-Enhancer-Binding Proteins)
0 (Cell Cycle Proteins)
0 (Chromatin)
0 (Transcription Factors)
EC 2.3.2.27 (Anaphase-Promoting Complex-Cyclosome)
EC 2.3.2.27 (UHRF1 protein, human)
EC 2.3.2.27 (Ubiquitin-Protein Ligases)
تواريخ الأحداث: Date Created: 20201211 Date Completed: 20210202 Latest Revision: 20210630
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7758050
DOI: 10.1371/journal.pbio.3000975
PMID: 33306668
قاعدة البيانات: MEDLINE
الوصف
تدمد:1545-7885
DOI:10.1371/journal.pbio.3000975