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IGI-LuNER: single-well multiplexed RT-qPCR test for SARS-CoV-2.

التفاصيل البيبلوغرافية
العنوان:	IGI-LuNER: single-well multiplexed RT-qPCR test for SARS-CoV-2.
المؤلفون:	Stahl EC; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Tsuchida CA; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Hamilton JR; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Lin-Shiao E; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., McDevitt SL; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Moehle EA; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Witkowsky LB; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Tsui CK; University of California, Berkeley, Berkeley, CA, USA., Pestal K; University of California, Berkeley, Berkeley, CA, USA., Gildea HK; University of California, Berkeley, Berkeley, CA, USA., McElroy M; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Keller A; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Sylvain I; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Williams C; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Hirsh A; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Ciling A; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Ehrenberg AJ; University of California, Berkeley, Berkeley, CA, USA., Urnov FD; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Ringeisen BR; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Giannikopoulos P; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA., Doudna JA; University of California, Berkeley, Berkeley, CA, USA.; Innovative Genomics Institute, University of California Berkeley, Berkeley, CA, USA.; Howard Hughes Medical Institute, University of California, Berkeley, CA, USA.
مؤلفون مشاركون:	IGI SARS-CoV-2 consortium
المصدر:	MedRxiv : the preprint server for health sciences [medRxiv] 2020 Dec 11. Date of Electronic Publication: 2020 Dec 11.
نوع المنشور:	Preprint
اللغة:	English
بيانات الدورية:	Country of Publication: United States NLM ID: 101767986 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: medRxiv Subsets: PubMed not MEDLINE
مستخلص:	Commonly used RT-qPCR-based SARS-CoV-2 diagnostics require 2-3 separate reactions or rely on detection of a single viral target, adding time and cost or risk of false-negative results. Currently, no test combines detection of widely used SARS-CoV-2 E- and N-gene targets and a sample control in a single, multiplexed reaction. We developed the IGI-LuNER RT-qPCR assay using the Luna Probe Universal One-Step RT-qPCR master mix with publicly available primers and probes to detect SARS-CoV-2 N gene, E gene, and human RNase P (NER). This combined, cost-effective test can be performed in 384-well plates with detection sensitivity suitable for clinical reporting, and will aid in future sample pooling efforts, thus improving throughput of SARS-CoV-2 detection. Competing Interests: Competing Interests: The Regents of the University of California have patents issued and pending for CRISPR technologies on which J.A.D. is an inventor. J.A.D. is a cofounder of Caribou Biosciences, Editas Medicine, Scribe Therapeutics, Intellia Therapeutics and Mammoth Biosciences. J.A.D. is a scientific advisory board member of Caribou Biosciences, Intellia Therapeutics, eFFECTOR Therapeutics, Scribe Therapeutics, Mammoth Biosciences, Synthego, Algen Biotechnologies, Felix Biosciences and Inari. J.A.D. is a Director at Johnson & Johnson and has research projects sponsored by Biogen, Pfizer, AppleTree Partners and Roche. F.D.U. is a co-founder of Tune Therapeutics. P.G. is a cofounder and Director at NewCo Health. P.G. is the CLIA Laboratory Director for Coral Genomics and 3DMed. The other authors declare no competing interests.
معلومات مُعتمدة:	F99 AG068343 United States AG NIA NIH HHS
تواريخ الأحداث:	Date Created: 20201217 Latest Revision: 20231019
رمز التحديث:	20231020
مُعرف محوري في PubMed:	PMC7743092
DOI:	10.1101/2020.12.10.20247338
PMID:	33330883
قاعدة البيانات:	MEDLINE

الوصف
DOI:	10.1101/2020.12.10.20247338