دورية أكاديمية
أعرض في EDS

Generation of transgene-free induced pluripotent stem cells from cardiac fibroblasts of goat embryos.

التفاصيل البيبلوغرافية
العنوان: Generation of transgene-free induced pluripotent stem cells from cardiac fibroblasts of goat embryos.
المؤلفون: Hanna M; Institute of Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany.; Department of physiology, Faculty of medicine (Kasr El-Aini) Cairo University, El-Maniel, Cairo 11451, Egypt., Sahito RGA; Institute of Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany., Rateb M; Department of physiology, Faculty of medicine (Kasr El-Aini) Cairo University, El-Maniel, Cairo 11451, Egypt., Kachiwal AB; Department of Veterinary Physiology and Biochemistry, Sindh Agriculture University Tandojam, Pakistan., Seddiek HA; Department of physiology, Faculty of medicine (Kasr El-Aini) Cairo University, El-Maniel, Cairo 11451, Egypt., Bhutto B; Department of Veterinary Parasitology, Sindh Agriculture University Tandojam, Pakistan., Hescheler J; Institute of Neurophysiology, University of Cologne, Robert-Koch-Strasse 39, 50931 Cologne, Germany.
المصدر: Journal of stem cells & regenerative medicine [J Stem Cells Regen Med] 2020 Dec 11; Vol. 16 (2), pp. 34-43. Date of Electronic Publication: 2020 Dec 11 (Print Publication: 2020).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: German Stem Cell Society Country of Publication: India NLM ID: 101552911 Publication Model: eCollection Cited Medium: Print ISSN: 0973-7154 (Print) Linking ISSN: 09737154 NLM ISO Abbreviation: J Stem Cells Regen Med Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: Chennai : German Stem Cell Society
Original Publication: [S.I.] : Journal of Stem Cells & Regenerative Medicine
مستخلص: Induced pluripotent stem cells (iPSCs) hold a great potential for therapeutic regenerative medicine. The aim of this study was to generate induced pluripotent stem cells from goat embryonic cardiac tissue derived fibroblasts. The isolated cardiac fibroblasts from the cardiac tissue of goat embryos were positive for alfa smooth muscle actin, vimentin and discoidin domain receptor2. From these cells, we generated transgene free iPSCs using piggyBac transposons / transposase using five transcription factors (Oct4, Sox2, Klf, Myc and Lin 28). The generated iPSCs were SSEA1, SSEA4 and Oct4 positive. They were cultured on neofeeders using 20% Serum replacement - IMDM with bFGF. They could form cystic and compact embryoid bodies that showed differentiated ectodermal and mesodermal like cells when cultured using 20% FBS-IMDM without bFGF. The iPSCs, generated in the frame of this approach were produced without the use of integrating virus and the reprogramming transgenes were removed at the end of the process. Though there were limitations in the approach used, a substantial sign of reprogramming was obtained.
Competing Interests: None
(Copyright © Journal of Stem Cells and Regenerative Medicine.)
References: Stem Cells. 2007 Feb;25(2):455-64. (PMID: 17038665)
Cell. 2006 Aug 25;126(4):663-76. (PMID: 16904174)
PLoS One. 2010 Mar 08;5(3):e9580. (PMID: 20221450)
Hum Reprod. 2009 Jun;24(6):1359-72. (PMID: 19251728)
PLoS One. 2013;8(1):e52481. (PMID: 23326334)
PLoS Genet. 2016 Aug 24;12(8):e1006243. (PMID: 27556156)
PLoS One. 2014 Feb 05;9(2):e88346. (PMID: 24505480)
Int J Mol Sci. 2014 Mar 20;15(3):5011-31. (PMID: 24658443)
Stem Cells Dev. 2013 Feb 1;22(3):473-82. (PMID: 22889279)
Nature. 2009 Apr 9;458(7239):766-70. (PMID: 19252478)
J Cell Biochem. 2012 Oct;113(10):3061-8. (PMID: 22573568)
Cell Res. 2011 Oct;21(10):1509-12. (PMID: 21826109)
Mol Reprod Dev. 2013 Dec;80(12):1009-17. (PMID: 24123501)
Am J Physiol. 1995 Apr;268(4 Pt 2):H1757-64. (PMID: 7733380)
Annu Rev Pharmacol Toxicol. 2005;45:657-87. (PMID: 15822192)
Biol Open. 2013 May 10;2(6):613-28. (PMID: 23789112)
Biol Reprod. 2005 Dec;73(6):1147-56. (PMID: 16079311)
Front Cell Dev Biol. 2015 Feb 02;3:2. (PMID: 25699255)
Elife. 2015 Jun 23;4:e07103. (PMID: 26102527)
Nat Methods. 2009 May;6(5):363-9. (PMID: 19337237)
Cell Res. 2011 May;21(5):849-53. (PMID: 21403680)
Microsc Microanal. 2011 Aug;17(4):474-97. (PMID: 21682936)
Mol Reprod Dev. 2015 Sep;82(9):709-21. (PMID: 26118622)
Sheng Wu Gong Cheng Xue Bao. 2008 Sep;24(9):1670-6. (PMID: 19160855)
J Biomed Sci. 2009 Sep 22;16:88. (PMID: 19772618)
J Mol Cell Biol. 2009 Oct;1(1):46-54. (PMID: 19502222)
Curr Protoc Stem Cell Biol. 2012;Chapter 4:Unit4A.5. (PMID: 22605648)
Cell. 2007 Nov 30;131(5):861-72. (PMID: 18035408)
Dev Dyn. 2010 Jun;239(6):1573-84. (PMID: 20503355)
Circ Res. 2009 Dec 4;105(12):1164-76. (PMID: 19959782)
Cardiovasc Res. 2003 May 1;58(2):423-34. (PMID: 12757876)
Cell Mol Life Sci. 2018 Apr;75(7):1191-1203. (PMID: 29134247)
Hum Reprod. 2007 May;22(5):1231-8. (PMID: 17251355)
Circ Res. 2014 Apr 25;114(9):1422-34. (PMID: 24650916)
J Clin Invest. 2010 Apr;120(4):1125-39. (PMID: 20335662)
Stem Cell Rev Rep. 2011 Sep;7(3):693-702. (PMID: 21347602)
In Vitro Cell Dev Biol Anim. 1994 Jul;30A(7):420-4. (PMID: 7952510)
EMBO J. 2008 Apr 9;27(7):1097-109. (PMID: 18354502)
J Anim Sci. 2011 Sep;89(9):2708-16. (PMID: 21478453)
J Biosci Bioeng. 2009 Apr;107(4):442-6. (PMID: 19332306)
Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1531-6. (PMID: 21205896)
Nature. 2009 Apr 9;458(7239):771-5. (PMID: 19252477)
Circ Heart Fail. 2015 Jan;8(1):156-66. (PMID: 25420485)
Stem Cells. 2007 Jan;25(1):54-62. (PMID: 17008424)
Science. 2007 Dec 21;318(5858):1917-20. (PMID: 18029452)
Nat Biotechnol. 2006 Mar;24(3):344-50. (PMID: 16444268)
Am J Physiol Regul Integr Comp Physiol. 2007 Nov;293(5):R1898-907. (PMID: 17686880)
PLoS One. 2011;6(9):e24501. (PMID: 21912700)
Cell Biochem Biophys. 2013 Jun;66(2):309-18. (PMID: 23212180)
فهرسة مساهمة: Keywords: Cardiac fibroblast; Integration-Free nuclear reprogramming; PSCs; Pluripotency markers; Transcription factors
تواريخ الأحداث: Date Created: 20210108 Latest Revision: 20210111
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC7772807
DOI: 10.46582/jsrm.1602007
PMID: 33414579
قاعدة البيانات: MEDLINE
الوصف
تدمد:0973-7154
DOI:10.46582/jsrm.1602007