دورية أكاديمية
Neurochemical regulation of Aedes aegypti salivary gland function.
| العنوان: | Neurochemical regulation of Aedes aegypti salivary gland function. |
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| المؤلفون: | Soohoo-Hui A; Louisiana State University AgCenter, Department of Entomology, Baton Rouge, LA 70803, USA., Li Z; Louisiana State University AgCenter, Department of Entomology, Baton Rouge, LA 70803, USA., Maldonado-Ruiz LP; Department of Entomology, Kansas State University, Manhattan, KS 66506, USA., Zhang G; Louisiana State University AgCenter, Department of Entomology, Baton Rouge, LA 70803, USA; Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China., Swale DR; Louisiana State University AgCenter, Department of Entomology, Baton Rouge, LA 70803, USA. Electronic address: dswale@agcenter.lsu.edu. |
| المصدر: | Journal of insect physiology [J Insect Physiol] 2021 Feb-Mar; Vol. 129, pp. 104193. Date of Electronic Publication: 2021 Jan 16. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 2985080R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1611 (Electronic) Linking ISSN: 00221910 NLM ISO Abbreviation: J Insect Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Elsevier Original Publication: London ; New York : Pergamon Press |
| مواضيع طبية MeSH: | Saliva*/chemistry , Saliva*/drug effects , Salivary Glands*/drug effects , Salivary Glands*/physiology, Aedes/*physiology , Neurotransmitter Agents/*pharmacology, Amylases/drug effects ; Amylases/metabolism ; Animals ; Apyrase/drug effects ; Apyrase/metabolism ; Dopamine/pharmacology ; Female ; Humans ; Insect Proteins/drug effects ; Pilocarpine/pharmacology ; Rats ; Receptors, Dopamine D1 ; Serotonin/pharmacology |
| مستخلص: | The salivary gland of hematophagous arthropods is critical for blood meal acquisition, blood vessel localization, and secretion of digestive enzymes. Thus, there is significant interest in the regulation of salivary gland function and mechanisms driving the secretion of saliva and digestive proteins. We aimed to gain a broader understanding of the regulatory role of aminergic, cholinergic, and octopaminergic neuromodulators to saliva and protein secretion from the female A. aegypti salivary gland. Quantification of saliva after injection with neuromodulators showed that dopamine, serotonin, and pilocarpine increased the secretory activity of the salivary gland with potency rankings dopamine = serotonin > pilocarpine. No change in saliva secretion was observed with octopamine or ergonovine, which indicates the A. aegypti salivary gland may be regulated by dopaminergic, serotonergic, and cholinergic systems, but are not likely regulated by octopaminergic or tryptaminergic systems. Next, we studied the regulatory control of dopamine-mediated salivation. Data indicate extracellular calcium flux, but not neural function, is critical for dopamine-mediated salivation, which suggests epithelial transport of ions and not neuronal control is responsible for dopamine-mediated salivation. For regulation of protein secretion, data indicate dopamine or serotonin exposure facilitates amylase secretion, whereas serotonin but not dopamine exposure increased apyrase concentrations in the secreted saliva. General immunoreactivity to anti-rat D1-dopamine receptor antibody was observed, yet immunoreactivity to the anti-rat D2-receptor antibody was identified in the proximal regions of the lateral lobes and slight immunoreactivity in the distal portion of the lateral lobe, with no expression in the medial lobe. (Copyright © 2021 Elsevier Ltd. All rights reserved.) |
| معلومات مُعتمدة: | CT-0273 USDA Hatch |
| فهرسة مساهمة: | Keywords: Amylase; Apyrase; Dopamine; Mosquito; Neurohormone; Salivary gland; Salivation; Serotonin |
| المشرفين على المادة: | 0 (DRD1 protein, human) 0 (Insect Proteins) 0 (Neurotransmitter Agents) 0 (Receptors, Dopamine D1) 01MI4Q9DI3 (Pilocarpine) 333DO1RDJY (Serotonin) EC 3.2.1.- (Amylases) EC 3.6.1.5 (Apyrase) VTD58H1Z2X (Dopamine) |
| تواريخ الأحداث: | Date Created: 20210118 Date Completed: 20210920 Latest Revision: 20210920 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jinsphys.2021.104193 |
| PMID: | 33460707 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-1611 |
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| DOI: | 10.1016/j.jinsphys.2021.104193 |