دورية أكاديمية
Giardia intestinalis coiled-coil cytolinker protein 259 interacts with actin and tubulin.
| العنوان: | Giardia intestinalis coiled-coil cytolinker protein 259 interacts with actin and tubulin. |
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| المؤلفون: | Rojas-Gutiérrez O; Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, C.P. 11340, Ciudad de México, Mexico., Pérez-Rangel A; Departamento de Biología Celular, CINVESTAV, IPN, Av. IPN 2508, C.P. 07300, Ciudad de México, Mexico., Castillo-Romero A; Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico., Tapia-Ramírez J; Departamento de Genética y Biología Molecular, CINVESTAV, IPN, Av. IPN 2508, C.P. 07300, Ciudad de México, Mexico., Ríos-Castro E; Unidad de Genómica, Proteómica y Metabolómica, LaNSE, CINVESTAV-IPN, Av. IPN 2508, C.P. 07300, Ciudad de México, Mexico., Camacho-Nuez M; Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, San Lorenzo 290, C.P. 03100, Ciudad de México, Mexico., Giono-Cerezo S; Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, C.P. 11340, Ciudad de México, Mexico., Nogueda-Torres B; Departamento de Parasitología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, C.P. 11340, Ciudad de México, Mexico., León-Avila G; Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala S/N, C.P. 11340, Ciudad de México, Mexico. leonavila60@yahoo.com.mx., Hernández JM; Departamento de Biología Celular, CINVESTAV, IPN, Av. IPN 2508, C.P. 07300, Ciudad de México, Mexico. manolo@cell.cinvestav.mx. |
| المصدر: | Parasitology research [Parasitol Res] 2021 Mar; Vol. 120 (3), pp. 1067-1076. Date of Electronic Publication: 2021 Jan 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Country of Publication: Germany NLM ID: 8703571 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-1955 (Electronic) Linking ISSN: 09320113 NLM ISO Abbreviation: Parasitol Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin : Springer International, c1987- |
| مواضيع طبية MeSH: | Actins/*metabolism , Giardia lamblia/*metabolism , Plakins/*metabolism , Tubulin/*metabolism, Actins/chemistry ; Amino Acid Sequence ; Animals ; Ankyrins/chemistry ; Base Sequence ; Blotting, Western ; Computational Biology ; Consensus Sequence ; Cytoplasm/chemistry ; Cytoskeleton/chemistry ; Cytoskeleton/physiology ; Cytoskeleton/ultrastructure ; Dynamins/analysis ; Female ; Fluorescent Antibody Technique ; Giardia lamblia/chemistry ; Giardia lamblia/ultrastructure ; Humans ; Immunoprecipitation ; Mice ; Mice, Inbred BALB C ; Microscopy, Confocal ; Plakins/chemistry ; Sequence Alignment ; Tubulin/chemistry |
| مستخلص: | Giardia intestinalis is a human parasite that causes a diarrheal disease in developing countries. G. intestinalis has a cytoskeleton (CSK) composed of microtubules and microfilaments, and the Giardia genome does not code for the canonical CSK-binding proteins described in other eukaryotic cells. To identify candidate actin and tubulin cross-linking proteins, we performed a BLAST analysis of the Giardia genome using a spectraplakins consensus sequence as a query. Based on the highest BLAST score, we selected a 259-kDa sequence designated as a cytoskeleton linker protein (CLP259). The sequence was cloned in three fragments and characterized by immunoprecipitation, confocal microscopy, and mass spectrometry (MS). CLP259 was located in the cytoplasm in the form of clusters of thick rods and colocalized with actin at numerous sites and with tubulin in the median body. Immunoprecipitation followed by mass spectrometry revealed that CLP259 interacts with structural proteins such as giardins, SALP-1, axonemal, and eight coiled-coils. The vesicular traffic proteins detected were Mu adaptin, Vacuolar ATP synthase subunit B, Bip, Sec61 alpha, NSF, AP complex subunit beta, and dynamin. These results indicate that CLP259 in trophozoites is a CSK linker protein for actin and tubulin and could act as a scaffold protein driving vesicular traffic. |
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| معلومات مُعتمدة: | 180016 CONACyT; 20200344 SIP-IPN |
| فهرسة مساهمة: | Keywords: Coiled-coil; Cytoskeleton; Giardia intestinalis; Vesicular traffic |
| المشرفين على المادة: | 0 (Actins) 0 (Ankyrins) 0 (Plakins) 0 (Tubulin) EC 3.6.5.5 (Dynamins) |
| تواريخ الأحداث: | Date Created: 20210130 Date Completed: 20210622 Latest Revision: 20210622 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s00436-021-07062-6 |
| PMID: | 33515065 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1432-1955 |
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| DOI: | 10.1007/s00436-021-07062-6 |