دورية أكاديمية
أعرض في EDS

A high leukocyte count and administration of hydrocortisone hamper PCR-based diagnostics for bloodstream infections.

التفاصيل البيبلوغرافية
العنوان: A high leukocyte count and administration of hydrocortisone hamper PCR-based diagnostics for bloodstream infections.
المؤلفون: Huber S; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria., Weinberger J; Ares Genetics GmbH, Karl-Farkas-Gasse 18, 1030, Vienna, Austria., Pilecky M; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria., Lorenz I; Department of General and Surgical Critical Care Medicine, Medical University of Innsbruck, Innsbruck, Austria., Schildberger A; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria., Weber V; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.; Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis, Danube University Krems, Krems, Austria., Fuchs S; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria., Posch W; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria., Knabl L; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria., Würzner R; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria., Posch AE; Ares Genetics GmbH, Karl-Farkas-Gasse 18, 1030, Vienna, Austria. andreas.posch@ares-genetics.com., Orth-Höller D; Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Schöpfstrasse 41, 6020, Innsbruck, Austria. do@mb-lab.com.
المصدر: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2021 Jul; Vol. 40 (7), pp. 1441-1449. Date of Electronic Publication: 2021 Feb 05.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: Germany NLM ID: 8804297 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1435-4373 (Electronic) Linking ISSN: 09349723 NLM ISO Abbreviation: Eur J Clin Microbiol Infect Dis Subsets: MEDLINE
أسماء مطبوعة: Publication: Berlin : Springer
Original Publication: [Wiesbaden, Federal Republic of Germany] : Vieweg, [c1988-
مواضيع طبية MeSH: Hydrocortisone/*administration & dosage , Molecular Diagnostic Techniques/*methods , Polymerase Chain Reaction/*methods , Sepsis/*microbiology, Adolescent ; Adult ; Aged ; Blood Culture/methods ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Leukocyte Count ; Male ; Middle Aged ; Multiplex Polymerase Chain Reaction ; Retrospective Studies ; Sensitivity and Specificity ; Young Adult
مستخلص: Bloodstream infections (BSIs) require an accurate and fast identification of causative pathogens. Molecular diagnostics, in particular polymerase chain reaction (PCR)-based approaches for BSI diagnostics directly from whole blood, suffer from limitations such as inhibition leading to invalid results. In this retrospective study, we analyzed 23 parameters for their potential interference with LightCycler SeptiFast PCR tests (n = 2167) routinely performed at our institution. The overall inhibition rate was 9.1%. Test date, type of ward, procalcitonin levels, high leukocyte counts, and absolute neutrophil count were significantly associated with inhibition. For a subset (n = 448), cut-off values for leukocyte counts of < 5700 cells/μL and ≥ 26,900 cells/μL were significantly associated with a low (5%) and high (67%) inhibition risk. For patients with a moderate to high leukocyte count (5700-26,900 cells/μL), the additional administration of hydrocortisone significantly increased the inhibition risk. Furthermore, freezing of blood samples prior to DNA extraction and SF testing appeared to neutralize inhibitory factors. It remains to be investigated whether other molecular diagnostic tests are susceptible to similar inhibiting parameters.
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معلومات مُعتمدة: 871243 Österreichische Forschungsförderungsgesellschaft
فهرسة مساهمة: Keywords: Bloodstream infection; Inhibition; Leukocytosis; Molecular diagnostics; Multiplex PCR; Sepsis
المشرفين على المادة: WI4X0X7BPJ (Hydrocortisone)
تواريخ الأحداث: Date Created: 20210206 Date Completed: 20211008 Latest Revision: 20220304
رمز التحديث: 20231215
DOI: 10.1007/s10096-020-04126-w
PMID: 33547522
قاعدة البيانات: MEDLINE
الوصف
تدمد:1435-4373
DOI:10.1007/s10096-020-04126-w