دورية أكاديمية
Volatile Organic Compound-Mediated Antifungal Activity of Pichia spp. and Its Effect on the Metabolic Profiles of Fermentation Communities.
| العنوان: | Volatile Organic Compound-Mediated Antifungal Activity of Pichia spp. and Its Effect on the Metabolic Profiles of Fermentation Communities. |
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| المؤلفون: | Zhang H; Key Laboratory of Industrial Biotechnology of Ministry of Education, Synergetic Innovation Center of Food Safety and Nutrition, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China., Du H; Key Laboratory of Industrial Biotechnology of Ministry of Education, Synergetic Innovation Center of Food Safety and Nutrition, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China duhai88@126.com yxu@jiangnan.edu.cn., Xu Y; Key Laboratory of Industrial Biotechnology of Ministry of Education, Synergetic Innovation Center of Food Safety and Nutrition, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China duhai88@126.com yxu@jiangnan.edu.cn. |
| المصدر: | Applied and environmental microbiology [Appl Environ Microbiol] 2021 Apr 13; Vol. 87 (9). Date of Electronic Publication: 2021 Apr 13 (Print Publication: 2021). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7605801 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5336 (Electronic) Linking ISSN: 00992240 NLM ISO Abbreviation: Appl Environ Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Biological Control Agents/*metabolism , Ethanol/*metabolism , Monascus/*growth & development , Pichia/*metabolism , Volatile Organic Compounds/*metabolism, Alcoholic Beverages ; Bacteria/metabolism ; DNA Damage ; DNA, Fungal ; Fermentation ; Metabolome ; Monascus/genetics ; Protein Biosynthesis |
| مستخلص: | Volatile organic compounds (VOCs) are chemicals responsible for antagonistic activity between microorganisms. The impact of VOCs on microbial community succession of fermentation is not well understood. In this study, Pichia spp. were evaluated for VOC production as a part of antifungal activity during baijiu fermentation. The results showed that the abundance of Pichia in the defect group (agglomerated fermented grains) was lower than that in control group, and a negative interaction between Pichia and Monascus was determined ( P < 0.05). In addition, the disruption of fungi was significantly related to the differences of metabolic profiles in fermented grains. To determine production of VOCs from Pichia and its effect on Monascus purpureus , a double-dish system was assessed, and the incidence of M. purpureus reduction was 39.22% after 7 days. As to antifungal volatile compounds, 2-phenylethanol was identified to have an antifungal effect on M. purpureus through contact and noncontact. To further confirm the antifungal activity of 2-phenylethanol, scanning electron microscopy showed that 2-phenylethanol widely and significantly inhibited conidium germination and mycelial growth of filamentous fungi. Metatranscriptomic analysis revealed that the Ehrlich pathway is the metabolic path of 2-phenylethanol in Pichia and identified potential antifungal mechanisms, including protein synthesis and DNA damage. This study demonstrated the role of volatile compound-mediated microbial interaction in microbiome assembly and discovered a plausible scenario in which Pichia antagonized fungal blooms. The results may improve the niche establishment and growth of the functional yeast that enhances the flavor of baijiu. IMPORTANCE Fermentation of food occurs within communities of interacting species. The importance of microbial interactions in shaping microbial structure and metabolic performance to optimize the traditional fermentation process has long been emphasized, but the interaction mechanisms remain unclear. This study applied metabolome analysis and amplicon sequencing along with metatranscriptomic analysis to examine the volatile organic compound-mediated antifungal activity of Pichia and its effect on the metabolism of ethanol during baijiu fermentation, potentially enhancing the establishment of the fermentation niche and improving ethanol metabolism. (Copyright © 2021 American Society for Microbiology.) |
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| فهرسة مساهمة: | Keywords: 2-phenylethanol; biocontrol; metatranscriptomic analysis; volatile organic compounds; yeasts |
| المشرفين على المادة: | 0 (Biological Control Agents) 0 (DNA, Fungal) 0 (Volatile Organic Compounds) 3K9958V90M (Ethanol) |
| تواريخ الأحداث: | Date Created: 20210220 Date Completed: 20210430 Latest Revision: 20211015 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC8091005 |
| DOI: | 10.1128/AEM.02992-20 |
| PMID: | 33608301 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5336 |
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| DOI: | 10.1128/AEM.02992-20 |