دورية أكاديمية
Surface plasmon resonance biosensor for the detection of phenylethanolamine A in swine urine.
| العنوان: | Surface plasmon resonance biosensor for the detection of phenylethanolamine A in swine urine. |
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| المؤلفون: | Fan S; Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang, 050227, China. snowwinglv@126.com., Li C; Biology Institute of Hebei Academy of Sciences, Shijiazhuang, 050051, China., Ma J; Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang, 050227, China. snowwinglv@126.com., Meng Z; Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang, 050227, China. snowwinglv@126.com., Zhao L; Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang, 050227, China. snowwinglv@126.com., Zhang Y; Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang, 050227, China. snowwinglv@126.com and Hebei Key Laboratory of Forensic Medicine, College of Forensic Medicine, Hebei Medical University, Shijiazhuang, 050017, China. |
| المصدر: | Analytical methods : advancing methods and applications [Anal Methods] 2021 Mar 18; Vol. 13 (10), pp. 1278-1285. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: RSC Pub Country of Publication: England NLM ID: 101519733 Publication Model: Print Cited Medium: Internet ISSN: 1759-9679 (Electronic) Linking ISSN: 17599660 NLM ISO Abbreviation: Anal Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge : RSC Pub., 2009- |
| مواضيع طبية MeSH: | 2-Hydroxyphenethylamine*/analogs & derivatives , Biosensing Techniques*, Animals ; Gold ; Surface Plasmon Resonance ; Swine |
| مستخلص: | In the present study, an antibody against phenylethanolamine A (PEA) was produced, confirmed, and used in a surface plasmon resonance (SPR)-based measurement. Bovine serum albumin (BSA)-conjugated PEA was linked to nano-gold particles bound to l-cysteine modified on the surface of a Au-NP sensor chip. The concentrations of antigen and antibody were optimized, and the designed biosensor chip was investigated to examine the stability and accuracy of the proposed method. The recovery of PEA ranged from 80.4-93.4% in swine urine samples with spike levels of 5, 10 and 20 ng mL-1, and the relative standard deviations of PEA were less than 2%. PEA analogues, such as clenbuterol, ractopamine, and salbutamol, did not influence the PEA measurement. The developed method could be used to measure PEA in swine urine samples. |
| المشرفين على المادة: | 0 (2-(4-(nitrophenyl)butan-2-ylamino)-1-(4-methoxyphenyl)ethanol) 7440-57-5 (Gold) 7568-93-6 (2-Hydroxyphenethylamine) |
| تواريخ الأحداث: | Date Created: 20210224 Date Completed: 20210514 Latest Revision: 20210514 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1039/d1ay00132a |
| PMID: | 33624658 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1759-9679 |
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| DOI: | 10.1039/d1ay00132a |