دورية أكاديمية
أعرض في EDS

Proposed virulence-associated genes of Streptococcus suis isolates from the United States serve as predictors of pathogenicity.

التفاصيل البيبلوغرافية
العنوان: Proposed virulence-associated genes of Streptococcus suis isolates from the United States serve as predictors of pathogenicity.
المؤلفون: Estrada AA; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA. aquin033@umn.edu., Gottschalk M; Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, Quebec, Canada., Rendahl A; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA., Rossow S; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA., Marshall-Lund L; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA., Marthaler DG; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA., Gebhart CJ; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA.
المصدر: Porcine health management [Porcine Health Manag] 2021 Feb 28; Vol. 7 (1), pp. 22. Date of Electronic Publication: 2021 Feb 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101684126 Publication Model: Electronic Cited Medium: Print ISSN: 2055-5660 (Print) Linking ISSN: 20555660 NLM ISO Abbreviation: Porcine Health Manag Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2015]-
مستخلص: Background: There is limited information on the distribution of virulence-associated genes (VAGs) in U.S. Streptococcus suis isolates, resulting in little understanding of the pathogenic potential of these isolates. This lack also reduces our understanding of the epidemiology associated with S. suis in the United States and thus affects the efficiency of control and prevention strategies. In this study we applied whole genome sequencing (WGS)-based approaches for the characterization of S. suis and identification of VAGs.
Results: Of 208 S. suis isolates classified as pathogenic, possibly opportunistic, and commensal pathotypes, the genotype based on the classical VAGs (epf, mrp, and sly encoding the extracellular protein factor, muramidase-release protein, and suilysin, respectively) was identified in 9% (epf+/mrp+/sly+) of the pathogenic pathotype. Using the chi-square test and LASSO regression model, the VAGs ofs (encoding the serum opacity factor) and srtF (encoding sortase F) were selected out of 71 published VAGs as having a significant association with pathotype, and both genes were found in 95% of the pathogenic pathotype. The ofs+/srtF+ genotype was also present in 74% of 'pathogenic' isolates from a separate validation set of isolates. Pan-genome clustering resulted in the differentiation of a group of isolates from five swine production companies into clusters corresponding to clonal complex (CC) and virulence-associated (VA) genotypes. The same CC-VA genotype patterns were identified in multiple production companies, suggesting a lack of association between production company, CC, or VA genotype.
Conclusions: The proposed ofs and srtF genes were stronger predictors for differentiating pathogenic and commensal S. suis isolates compared to the classical VAGs in two sets of U.S. isolates. Pan-genome analysis in combination with metadata (serotype, ST/CC, VA genotype) was illustrated to be a valuable subtyping tool to describe the genetic diversity of S. suis.
References: J Med Microbiol. 2014 Jun;63(Pt 6):824-830. (PMID: 24696517)
Bioinformatics. 2015 Nov 15;31(22):3691-3. (PMID: 26198102)
Vet Microbiol. 2013 Mar 23;162(2-4):842-849. (PMID: 23245487)
BMC Microbiol. 2016 Jul 22;16(1):162. (PMID: 27449127)
Future Microbiol. 2012 Feb;7(2):259-79. (PMID: 22324994)
Vet Microbiol. 2000 Jun 1;74(3):237-48. (PMID: 10808092)
Emerg Infect Dis. 2017 Feb;23(2):204-211. (PMID: 27997331)
Vet Microbiol. 2006 Jun 15;115(1-3):117-27. (PMID: 16431041)
Vet Microbiol. 2013 Mar 23;162(2-4):819-825. (PMID: 23177911)
Nat Commun. 2015 Mar 31;6:6740. (PMID: 25824154)
PLoS One. 2010 Jan 05;5(1):e8426. (PMID: 20052283)
J Clin Microbiol. 2019 Jun 25;57(7):. (PMID: 30944194)
Can J Vet Res. 2007 Jan;71(1):14-22. (PMID: 17193877)
J Clin Microbiol. 2019 Aug 26;57(9):. (PMID: 31243086)
J Stat Softw. 2010;33(1):1-22. (PMID: 20808728)
Vet Microbiol. 2004 Jun 3;100(3-4):255-68. (PMID: 15145504)
FEMS Microbiol Lett. 2017 Dec 1;364(22):. (PMID: 29029051)
Pathogens. 2020 May 14;9(5):. (PMID: 32422856)
J Vet Diagn Invest. 1995 Jul;7(3):405-6. (PMID: 7578463)
Epidemiol Infect. 1994 Oct;113(2):321-34. (PMID: 7925669)
Genome Med. 2014 Nov 20;6(11):90. (PMID: 25422674)
J Clin Microbiol. 2014 May;52(5):1714-9. (PMID: 24574288)
J Vet Diagn Invest. 1992 Apr;4(2):195-6. (PMID: 1616988)
Emerg Microbes Infect. 2014 Jun;3(6):e45. (PMID: 26038745)
Pathogens. 2016 Jul 11;5(3):. (PMID: 27409640)
J Clin Microbiol. 2002 Feb;40(2):615-9. (PMID: 11825980)
FEMS Microbiol Lett. 2017 Jan;364(2):. (PMID: 28011701)
Pathogens. 2020 Jan 27;9(2):. (PMID: 32012668)
Can Vet J. 2001 Mar;42(3):223. (PMID: 11265194)
Can J Vet Res. 2016 Apr;80(2):106-11. (PMID: 27127337)
Infect Immun. 2006 Nov;74(11):6154-62. (PMID: 17057090)
Vet Microbiol. 2005 Apr 25;107(1-2):63-9. (PMID: 15795078)
Can J Vet Res. 2015 Oct;79(4):343-6. (PMID: 26424917)
One Health Outlook. 2020 Feb 18;2:3. (PMID: 33829127)
Vet Microbiol. 2000 Oct 1;76(3):259-72. (PMID: 10973700)
PLoS One. 2015 Sep 16;10(9):e0137760. (PMID: 26375680)
BMC Microbiol. 2011 Jul 07;11:161. (PMID: 21736719)
BMC Genomics. 2011 Oct 25;12:523. (PMID: 22026465)
Ann Agric Environ Med. 2017 Dec 23;24(4):683-695. (PMID: 29284248)
Int J Food Microbiol. 2018 Dec 20;287:3-9. (PMID: 29246458)
J Clin Microbiol. 2002 Oct;40(10):3671-80. (PMID: 12354864)
Can J Vet Res. 1990 Jan;54(1):174-7. (PMID: 2306669)
J Clin Microbiol. 1999 Feb;37(2):362-6. (PMID: 9889219)
Trends Microbiol. 2017 Jul;25(7):585-599. (PMID: 28274524)
Methods. 2016 Jun 1;102:3-11. (PMID: 27012178)
Microbiology (Reading). 2019 Feb;165(2):163-173. (PMID: 30543506)
Can J Vet Res. 2002 Oct;66(4):240-8. (PMID: 12418779)
FEBS Lett. 2016 Nov;590(21):3772-3799. (PMID: 27539145)
Sci Rep. 2016 May 27;6:26966. (PMID: 27231021)
Porcine Health Manag. 2020 May 06;6:12. (PMID: 32391166)
Infect Immun. 1996 Oct;64(10):4409-12. (PMID: 8926123)
Can J Vet Res. 1994 Jul;58(3):220-3. (PMID: 7954125)
Vet Microbiol. 2009 Nov 18;139(3-4):310-7. (PMID: 19596529)
J Gen Microbiol. 1992 Dec;138(12):2639-45. (PMID: 1362584)
J Vet Diagn Invest. 1996 Jan;8(1):119-21. (PMID: 9026068)
PLoS One. 2008 May 07;3(5):e2080. (PMID: 18461172)
Pathogens. 2020 Jan 05;9(1):. (PMID: 31948073)
J Clin Microbiol. 2003 Jun;41(6):2498-502. (PMID: 12791872)
Transbound Emerg Dis. 2013 Nov;60 Suppl 2:69-79. (PMID: 24589104)
Implement Sci. 2019 Aug 13;14(1):79. (PMID: 31409417)
J Vet Diagn Invest. 1994 Jul;6(3):326-34. (PMID: 7948202)
Bioinformatics. 2013 Apr 15;29(8):1072-5. (PMID: 23422339)
Clin Microbiol Rev. 2017 Oct;30(4):1015-1063. (PMID: 28855266)
Curr Microbiol. 2017 Oct;74(10):1200-1209. (PMID: 28717847)
Clin Microbiol Infect. 2007 Oct;13 Suppl 3:1-46. (PMID: 17716294)
Front Microbiol. 2015 Sep 24;6:1001. (PMID: 26441928)
Sci Rep. 2016 Jul 06;6:28984. (PMID: 27381348)
Microb Pathog. 2003 Jan;34(1):27-37. (PMID: 12620382)
PLoS One. 2014 Nov 19;9(11):e112963. (PMID: 25409509)
J Am Vet Med Assoc. 1993 Jul 15;203(2):295-9. (PMID: 8407494)
Can J Vet Res. 1998 Jan;62(1):75-9. (PMID: 9442945)
Clin Vaccine Immunol. 2010 Oct;17(10):1589-97. (PMID: 20739502)
Vet Microbiol. 2009 May 28;137(1-2):196-201. (PMID: 19185432)
Can Vet J. 2019 Apr;60(4):386-390. (PMID: 30992594)
Infect Immun. 2001 Apr;69(4):2732-5. (PMID: 11254643)
J Clin Microbiol. 2001 Feb;39(2):445-53. (PMID: 11158088)
J Clin Microbiol. 2013 Aug;51(8):2582-91. (PMID: 23720795)
Emerg Infect Dis. 2011 Dec;17(12):2239-44. (PMID: 22172538)
Lancet Infect Dis. 2004 Jun;4(6):337-48. (PMID: 15172342)
FEMS Microbiol Lett. 1999 Sep 15;178(2):265-70. (PMID: 10499276)
Vet Rec. 1998 Oct 3;143(14):394-5. (PMID: 9802198)
Wellcome Open Res. 2018 Sep 24;3:124. (PMID: 30345391)
Bioinformatics. 2014 Jul 15;30(14):2068-9. (PMID: 24642063)
Can Vet J. 2008 May;49(5):461-2. (PMID: 18512456)
Future Microbiol. 2016 Jul;11:941-54. (PMID: 27357518)
Eur J Clin Microbiol Infect Dis. 2018 May;37(5):917-925. (PMID: 29417311)
J Clin Microbiol. 1991 Apr;29(4):782-7. (PMID: 1890177)
Acta Pathol Microbiol Immunol Scand B. 1987 Aug;95(4):233-9. (PMID: 3673579)
Curr Opin Genet Dev. 2005 Dec;15(6):589-94. (PMID: 16185861)
معلومات مُعتمدة: project #00057268 Rapid Agricultural Response Fund, Minnesota Agricultural Experiment Station (US)
فهرسة مساهمة: Keywords: Genetic diversity; Pan-genome; Pathotype; Streptococcus suis; Virulence-associated genes (VAGs)
تواريخ الأحداث: Date Created: 20210302 Latest Revision: 20240331
رمز التحديث: 20240331
مُعرف محوري في PubMed: PMC7917538
DOI: 10.1186/s40813-021-00201-6
PMID: 33648592
قاعدة البيانات: MEDLINE
الوصف
تدمد:2055-5660
DOI:10.1186/s40813-021-00201-6