دورية أكاديمية
أعرض في EDS

An implanted device enables in vivo monitoring of extracellular vesicle-mediated spread of pro-inflammatory mast cell response in mice.

التفاصيل البيبلوغرافية
العنوان: An implanted device enables in vivo monitoring of extracellular vesicle-mediated spread of pro-inflammatory mast cell response in mice.
المؤلفون: Vukman KV; Department of Genetics Cell- and Immunobiology Semmelweis University Budapest Hungary., Ferencz A; Department of Surgical Research and Techniques Semmelweis University Budapest Hungary., Fehér D; Department of Surgical Research and Techniques Semmelweis University Budapest Hungary., Juhos K; Department of Surgical Research and Techniques Semmelweis University Budapest Hungary., Lőrincz P; Department of Anatomy Cell and Developmental Biology Eötvös Loránd University Budapest Hungary., Visnovitz T; Department of Genetics Cell- and Immunobiology Semmelweis University Budapest Hungary., Koncz A; Department of Genetics Cell- and Immunobiology Semmelweis University Budapest Hungary., Pálóczi K; Department of Genetics Cell- and Immunobiology Semmelweis University Budapest Hungary., Seregélyes G; Department of Genetics Cell- and Immunobiology Semmelweis University Budapest Hungary., Försönits A; Department of Genetics Cell- and Immunobiology Semmelweis University Budapest Hungary., Khamari D; Department of Genetics Cell- and Immunobiology Semmelweis University Budapest Hungary., Galinsoga A; Department of Genetics Cell- and Immunobiology Semmelweis University Budapest Hungary., Drahos L; MS Proteomics Research Group Hungarian Academy of Sciences Institute of Organic Chemistry Budapest Hungary., Buzás EI; Department of Genetics Cell- and Immunobiology Semmelweis University Budapest Hungary.; MTA-SE Immune-Proteogenomics Extracellular Vesicle Research Group Budapest Hungary.; HCEMM-SE Extracellular Vesicle Research Group Budapest Hungary.
المصدر: Journal of extracellular vesicles [J Extracell Vesicles] 2020 Nov; Vol. 10 (1), pp. e12023. Date of Electronic Publication: 2021 Jan 09.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: United States NLM ID: 101610479 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2001-3078 (Print) Linking ISSN: 20013078 NLM ISO Abbreviation: J Extracell Vesicles
أسماء مطبوعة: Publication: 2020- : [Hoboken, NJ] : Wiley
Original Publication: Järfälla : Co-Action Pub.
مواضيع طبية MeSH: MAP Kinase Signaling System*, Extracellular Vesicles/*metabolism , Mast Cells/*metabolism, Animals ; Cells, Cultured ; Extracellular Vesicles/genetics ; Gene Expression Regulation/drug effects ; Inflammation/chemically induced ; Inflammation/genetics ; Inflammation/metabolism ; Lipopolysaccharides/toxicity ; Mice ; Mice, Transgenic ; Tumor Necrosis Factor-alpha/biosynthesis ; Tumor Necrosis Factor-alpha/genetics
مستخلص: Mast cells have been shown to release extracellular vesicles (EVs) in vitro. However, EV-mediated mast cell communication in vivo remains unexplored. Primary mast cells from GFP-transgenic and wild type mice, were grown in the presence or absence of lipopolysaccharide (LPS), and the secreted EVs were separated from the conditioned media. Mast cell-derived EVs were next cultured with LPS-naïve mast cells, and the induction of TNF-α expression was monitored. In addition, primary mast cells were seeded in diffusion chambers that were implanted into the peritoneal cavities of mice. Diffusion chambers enabled the release of GFP + mast cell-derived EVs in vivo into the peritoneal cavity. Peritoneal lavage cells were assessed for the uptake of GFP + EVs and for TNF-α production. In vitro, LPS-stimulated mast cell-derived EVs were efficiently taken up by non-stimulated mast cells, and induced TNF-α expression in a TLR4, JNK and P38 MAPK dependent manner. In vivo, using implanted diffusion chambers, we confirmed the release and transmission of mast cell-derived EVs to other mast cells with subsequent induction of TNF-α expression. These data show an EV-mediated spreading of pro-inflammatory response between mast cells, and provide the first in vivo evidence for the biological role of mast cell-derived EVs.
Competing Interests: The authors do not have financial interest to declare.
(© 2020 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.)
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فهرسة مساهمة: Keywords: LPS; TNF‐α; extracellular vesicles; in vitro; in vivo; mast cell
المشرفين على المادة: 0 (Lipopolysaccharides)
0 (Tumor Necrosis Factor-alpha)
تواريخ الأحداث: Date Created: 20210312 Date Completed: 20220118 Latest Revision: 20240330
رمز التحديث: 20240330
مُعرف محوري في PubMed: PMC7890545
DOI: 10.1002/jev2.12023
PMID: 33708356
قاعدة البيانات: MEDLINE
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