دورية أكاديمية
Aberrant Expression of Sodium-Potassium-Chloride Cotransporter in Endometriosis.
| العنوان: | Aberrant Expression of Sodium-Potassium-Chloride Cotransporter in Endometriosis. |
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| المؤلفون: | Lee I; Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea., Jeon MJ; Department of Obstetrics and Gynecology, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, South Korea., Kim JS; Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan, South Korea., Park JH; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea.; Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, 211 Eonju-ro, Gangnam-gu, Seoul, 06273, South Korea., Won BH; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea.; Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, 211 Eonju-ro, Gangnam-gu, Seoul, 06273, South Korea., Kim H; Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea., Lee JH; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea.; Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, 211 Eonju-ro, Gangnam-gu, Seoul, 06273, South Korea., Yun BH; Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea., Park JH; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea.; Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, 211 Eonju-ro, Gangnam-gu, Seoul, 06273, South Korea., Seo SK; Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea., Choi YS; Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea., Cho S; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea. sihyuncho@yuhs.ac.; Department of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, 211 Eonju-ro, Gangnam-gu, Seoul, 06273, South Korea. sihyuncho@yuhs.ac., Lee BS; Department of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea.; Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, South Korea. |
| المصدر: | Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2021 Sep; Vol. 28 (9), pp. 2641-2648. Date of Electronic Publication: 2021 Mar 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: United States NLM ID: 101291249 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1933-7205 (Electronic) Linking ISSN: 19337191 NLM ISO Abbreviation: Reprod Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [New York] : Springer Original Publication: Thousand Oaks, Calif. : Sage |
| مواضيع طبية MeSH: | Chloride Channels/*metabolism , Endometriosis/*metabolism , Endometrium/*metabolism , Solute Carrier Family 12, Member 1/*metabolism , Solute Carrier Family 12, Member 2/*metabolism , Stromal Cells/*metabolism, Adolescent ; Adult ; Case-Control Studies ; Cell Movement ; Cells, Cultured ; Chloride Channels/genetics ; Endometriosis/genetics ; Endometriosis/pathology ; Endometrium/pathology ; Female ; Humans ; Middle Aged ; Solute Carrier Family 12, Member 1/genetics ; Solute Carrier Family 12, Member 2/genetics ; Stromal Cells/pathology ; Up-Regulation ; Young Adult |
| مستخلص: | Cell membrane ion channels have important roles in cell migration during cancer development and metastasis. Although endometriosis is a benign gynecological disease, some migration and invasion characteristics of endometriosis are similar to those of cancer. However, only a few studies have examined cell membrane ion channels and their associations with endometriosis. This study aimed to investigate the effects of these ion channels on development of endometriosis. A total of 39 women who underwent laparoscopic ovarian cyst enucleation were included in the study population. Eutopic endometrium or ectopic endometrium tissues were obtained from each patient based on allocation to an endometriosis group (n=21) or a control group (n=18). Quantitative real-time PCR (qRT-PCR) and western blot analyses were performed to quantify NKCC1, NKCC2, and CLCN3 mRNA expression and protein concentrations. SiRNA transfection and migration assays of the endometrial stromal cells were performed to test the effects of the ion channels on the migration ability. The qRT-PCR and western blot analyses revealed significantly elevated mRNA expression and protein expression of NKCC1, NKCC2, and CLCN3 in the ectopic endometrial tissue from the patients with endometriosis (p < 0.05). Migration assay of siRNA transfected cells suggested a decreased migratory potential of the endometrial stromal cells (p < 0.001). The magnitudes of expression of NKCC1, NKCC2, and CLCN3 were positively correlated with endometrioma size. The increased expression of NKCC1, NKCC2, and CLCN3 in endometriosis offers opportunities to understand mechanisms of endometriosis and develop novel therapeutic approaches. (© 2021. Society for Reproductive Investigation.) |
| التعليقات: | Erratum in: Reprod Sci. 2021 Mar 24;:. (PMID: 33761126) |
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| فهرسة مساهمة: | Keywords: Cell migration; Endometriosis; Membrane channel; NKCC |
| المشرفين على المادة: | 0 (Chloride Channels) 0 (ClC-3 channel) 0 (SLC12A1 protein, human) 0 (SLC12A2 protein, human) 0 (Solute Carrier Family 12, Member 1) 0 (Solute Carrier Family 12, Member 2) |
| تواريخ الأحداث: | Date Created: 20210312 Date Completed: 20220125 Latest Revision: 20220125 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s43032-021-00531-4 |
| PMID: | 33709377 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. | تدمد: | 1933-7205 |
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| DOI: | 10.1007/s43032-021-00531-4 |