دورية أكاديمية
Experimental evolution of cowpea mild mottle virus reveals recombination-driven reduction in virulence accompanied by increases in diversity and viral fitness.
| العنوان: | Experimental evolution of cowpea mild mottle virus reveals recombination-driven reduction in virulence accompanied by increases in diversity and viral fitness. |
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| المؤلفون: | Zanardo LG; Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Trindade TA; Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Mar TB; Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Barbosa TMC; Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Milanesi DF; Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Alves MS; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Lima RRPN; Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Zerbini FM; Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Janssen A; Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa, MG, Brazil; IBED, University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands., Mizubuti ESG; Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Elliot SL; Departamento de Entomologia, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Carvalho CM; Departamento de Fitopatologia/BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, Brazil. Electronic address: claudine.carvalho@ufv.br. |
| المصدر: | Virus research [Virus Res] 2021 Oct 02; Vol. 303, pp. 198389. Date of Electronic Publication: 2021 Mar 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8410979 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7492 (Electronic) Linking ISSN: 01681702 NLM ISO Abbreviation: Virus Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier Science, c1984- |
| مواضيع طبية MeSH: | Carlavirus*/genetics , Hemiptera*/genetics , RNA Viruses*/genetics , Vigna*, Animals ; DNA Viruses/genetics ; RNA ; Recombination, Genetic ; Virulence/genetics |
| مستخلص: | Major themes in pathogen evolution are emergence, evolution of virulence, host adaptation and the processes that underlie them. RNA viruses are of particular interest due to their rapid evolution. The in vivo molecular evolution of an RNA plant virus was demonstrated here using a necrotic isolate of cowpea mild mottle virus (CPMMV) and a susceptible soybean genotype submitted to serial inoculations. We show that the virus lost the capacity to cause necrosis after six passages through the host plant. When a severe bottleneck was imposed, virulence reduction occurred in the second passage. The change to milder symptoms had fitness benefits for the virus (higher RNA accumulation) and for its vector, the whitefly Bemisia tabaci. Genetic polymorphisms were highest in ORF1 (viral replicase) and were independent of the symptom pattern. Recombination was a major contributor to this diversity - even with the strong genetic bottleneck, recombination events and hot spots were detected within ORF1. Virulence reduction was associated with different sites in ORF1 associated to recombination events in both experiments. Overall, the results demonstrate that the reduction in virulence was a consequence of the emergence of new variants, driven by recombination. Besides providing details of the evolutionary mechanisms behind a reduction in virulence and its effect under viral and vector fitness, we propose that this recombination-driven switch in virulence allows the pathogen to rapidly adapt to a new host and, potentially, switch back. (Copyright © 2021. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Bemisia tabaci; Carlavirus; Evolution of virulence; RNA virus; Recombination; Virus evolution |
| المشرفين على المادة: | 63231-63-0 (RNA) |
| تواريخ الأحداث: | Date Created: 20210315 Date Completed: 20220414 Latest Revision: 20220414 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.virusres.2021.198389 |
| PMID: | 33716182 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1872-7492 |
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| DOI: | 10.1016/j.virusres.2021.198389 |