دورية أكاديمية
أعرض في EDS

Comparative Analysis of Outer Membrane Vesicle Isolation Methods With an Escherichia coli tolA Mutant Reveals a Hypervesiculating Phenotype With Outer-Inner Membrane Vesicle Content.

التفاصيل البيبلوغرافية
العنوان: Comparative Analysis of Outer Membrane Vesicle Isolation Methods With an Escherichia coli tolA Mutant Reveals a Hypervesiculating Phenotype With Outer-Inner Membrane Vesicle Content.
المؤلفون: Reimer SL; Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada., Beniac DR; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada., Hiebert SL; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada., Booth TF; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada., Chong PM; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada., Westmacott GR; National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada., Zhanel GG; Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada., Bay DC; Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada.
المصدر: Frontiers in microbiology [Front Microbiol] 2021 Mar 05; Vol. 12, pp. 628801. Date of Electronic Publication: 2021 Mar 05 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Outer membrane vesicles (OMVs) produced by Gram-negative bacteria are mediators of cell survival and pathogenesis by facilitating virulence factor dissemination and resistance to antimicrobials. Studies of OMV properties often focus on hypervesiculating Escherichia coli mutants that have increased OMV production when compared to their corresponding wild-type (WT) strains. Currently, two conventional techniques, ultracentrifugation (UC) and ultradiafiltration (UF), are used interchangeably to isolate OMVs, however, there is concern that each technique may inadvertently alter the properties of isolated OMVs during study. To address this concern, we compared two OMV isolation methods, UC and UF, with respect to final OMV quantities, size distributions, and morphologies using a hypervesiculating Escherichia coli K-12 Δ tolA mutant. Nanoparticle tracking analysis (NTA) indicated that UC techniques result in lower vesicle yields compared to UF. However, UF permitted isolation of OMVs with smaller average sizes than UC, highlighting a potential OMV isolation size bias by each technique. Cryo-transmission electron microscopy (cryo-TEM) visualization of isolated OMVs revealed distinct morphological differences between WT and Δ tolA OMVs, where Δ tolA OMVs isolated by either UC or UF method possessed a greater proportion of OMVs with two or more membranes. Proteomic OMV analysis of WT and Δ tolA OMVs confirmed that Δ tolA enhances inner plasma membrane carryover in multi-lamellar OMVs. This study demonstrates that UC and UF are useful techniques for OMV isolation, where UF may be preferable due to faster isolation, higher OMV yields and enrichment of smaller sized vesicles.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Reimer, Beniac, Hiebert, Booth, Chong, Westmacott, Zhanel and Bay.)
References: Sci Rep. 2018 Mar 29;8(1):5402. (PMID: 29599474)
J Bacteriol. 2002 Feb;184(3):754-9. (PMID: 11790745)
Geobiology. 2008 Jun;6(3):214-9. (PMID: 18459967)
J Extracell Vesicles. 2014 Mar 25;3:. (PMID: 24678386)
PLoS One. 2015 Jan 12;10(1):e0116896. (PMID: 25581302)
Genes Dev. 1995 Feb 15;9(4):387-98. (PMID: 7883164)
J Bacteriol. 2005 May;187(10):3359-68. (PMID: 15866920)
Cell Microbiol. 2016 Nov;18(11):1525-1536. (PMID: 27673272)
Front Microbiol. 2020 Jan 17;10:3026. (PMID: 32038523)
J Extracell Vesicles. 2013 May 27;2:. (PMID: 24009894)
Microbiology (Reading). 2014 Oct;160(Pt 10):2109-2121. (PMID: 25069453)
Mol Microbiol. 2007 Jan;63(2):545-58. (PMID: 17163978)
Bioinformatics. 2010 Jul 1;26(13):1608-15. (PMID: 20472543)
J Bacteriol. 2006 Aug;188(15):5385-92. (PMID: 16855227)
Bioinformatics. 2009 Apr 15;25(8):1091-3. (PMID: 19237447)
J Extracell Vesicles. 2014 Jul 08;3:. (PMID: 25018865)
J Bacteriol. 1995 Jul;177(14):3998-4008. (PMID: 7608073)
J Extracell Vesicles. 2013 Feb 15;2:. (PMID: 24009893)
Appl Environ Microbiol. 2000 Oct;66(10):4414-20. (PMID: 11010892)
Pharm Res. 2010 May;27(5):796-810. (PMID: 20204471)
Microbiol Res. 2015 Jan;170:1-9. (PMID: 25458555)
J Proteome Res. 2019 Feb 1;18(2):623-632. (PMID: 30450911)
J Pathol. 1999 Jun;188(2):220-6. (PMID: 10398168)
J Bacteriol. 1998 Feb;180(4):831-9. (PMID: 9473036)
Helicobacter. 2015 Aug;20(4):269-83. (PMID: 25669590)
J Extracell Vesicles. 2015 Dec 23;4:29509. (PMID: 26700615)
Res Microbiol. 2013 Jun;164(5):397-405. (PMID: 23517882)
PLoS One. 2016 Dec 30;11(12):e0169186. (PMID: 28036403)
J Bacteriol. 2013 Jun;195(12):2755-67. (PMID: 23564175)
Anal Biochem. 2004 Mar 1;326(1):13-20. (PMID: 14769330)
Nat Commun. 2016 Jan 25;7:10515. (PMID: 26806181)
Vet Microbiol. 2013 Dec 27;167(3-4):565-72. (PMID: 24090812)
Mol Cell Proteomics. 2008 Mar;7(3):473-85. (PMID: 17982123)
J Bacteriol. 1998 Sep;180(18):4872-8. (PMID: 9733690)
Microbiologyopen. 2015 Jun;4(3):375-89. (PMID: 25755088)
Cell Microbiol. 2016 Nov;18(11):1508-1517. (PMID: 27529760)
Curr Protoc Cell Biol. 2006 Apr;Chapter 3:Unit 3.22. (PMID: 18228490)
Microbiol Spectr. 2019 Jan;7(1):. (PMID: 30681067)
Appl Environ Microbiol. 2013 Mar;79(6):1874-81. (PMID: 23315742)
FEMS Microbiol Lett. 1999 Aug 15;177(2):191-7. (PMID: 10474183)
Nat Rev Microbiol. 2015 Oct;13(10):605-19. (PMID: 26373371)
Proteomics. 2007 Sep;7(17):3143-53. (PMID: 17787032)
J Extracell Vesicles. 2017 Jun 19;6(1):1333883. (PMID: 28717425)
J Microbiol. 2012 Feb;50(1):155-60. (PMID: 22367951)
Can J Microbiol. 2018 Sep;64(9):589-599. (PMID: 30169125)
Proc Natl Acad Sci U S A. 2020 Mar 24;117(12):6777-6783. (PMID: 32152098)
Mol Microbiol. 2009 Jun;72(6):1395-407. (PMID: 19432795)
Nanomedicine. 2011 Dec;7(6):780-8. (PMID: 21601655)
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450. (PMID: 30395289)
Front Immunol. 2017 Aug 25;8:1017. (PMID: 28890719)
FEMS Microbiol Lett. 2009 Sep;298(1):1-11. (PMID: 19519769)
Front Microbiol. 2017 Jun 09;8:1053. (PMID: 28649237)
PLoS One. 2013 Dec 26;8(12):e83680. (PMID: 24386253)
J Extracell Vesicles. 2019 Jun 24;8(1):1632099. (PMID: 31275533)
Nat Methods. 2009 May;6(5):359-62. (PMID: 19377485)
J Bacteriol. 1996 Jul;178(14):4031-8. (PMID: 8763928)
PLoS Pathog. 2017 Feb 3;13(2):e1006159. (PMID: 28158302)
Mol Microbiol. 1991 May;5(5):1005-11. (PMID: 1683466)
Appl Environ Microbiol. 2020 Oct 1;86(20):. (PMID: 32801184)
Commun Biol. 2018 Apr 5;1:23. (PMID: 30271910)
J Biomed Sci. 2018 Nov 8;25(1):78. (PMID: 30409143)
Proteomics. 2014 Feb;14(2-3):222-9. (PMID: 24307187)
Res Microbiol. 2001 Jul-Aug;152(6):523-9. (PMID: 11501670)
J Bacteriol. 1995 Aug;177(15):4216-23. (PMID: 7635808)
J Biol Chem. 2000 Apr 28;275(17):12489-96. (PMID: 10777535)
Cell. 2003 Oct 3;115(1):25-35. (PMID: 14532000)
Front Cell Infect Microbiol. 2019 Sep 04;9:313. (PMID: 31552196)
PLoS One. 2015 Sep 25;10(9):e0139200. (PMID: 26406465)
Biotechnol J. 2015 Sep;10(11):1689-706. (PMID: 26912077)
Methods Mol Biol. 2013;966:259-72. (PMID: 23299740)
فهرسة مساهمة: Keywords: Escherichia coli; LC-MS/MS; Tol-Pal system; hypervesiculation; nanoparticle tracking analysis; outer membrane vesicles; ultracentrifugation; ultradiafiltration
تواريخ الأحداث: Date Created: 20210322 Latest Revision: 20210323
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7973035
DOI: 10.3389/fmicb.2021.628801
PMID: 33746922
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-302X
DOI:10.3389/fmicb.2021.628801