التفاصيل البيبلوغرافية
| العنوان: |
Rationally designed immunogens enable immune focusing to the SARS-CoV-2 receptor binding motif. |
| المؤلفون: |
Hauser BM, Sangesland M, Denis KJS, Windsor IW, Feldman J, Lam EC, Kannegieter T, Balazs AB, Lingwood D, Schmidt AG |
| المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2021 Jul 09. Date of Electronic Publication: 2021 Jul 09. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
Eliciting antibodies to surface-exposed viral glycoproteins can lead to protective responses that ultimately control and prevent future infections. Targeting functionally conserved epitopes may help reduce the likelihood of viral escape and aid in preventing the spread of related viruses with pandemic potential. One such functionally conserved viral epitope is the site to which a receptor must bind to facilitate viral entry. Here, we leveraged rational immunogen design strategies to focus humoral responses to the receptor binding motif (RBM) on the SARS-CoV-2 spike. Using glycan engineering and epitope scaffolding, we find an improved targeting of the serum response to the RBM in context of SARS-CoV-2 spike imprinting. Furthermore, we observed a robust SARS-CoV-2-neutralizing serum response with increased potency against related sarbecoviruses, SARS-CoV, WIV1-CoV, RaTG13-CoV, and SHC014-CoV. Thus, RBM focusing is a promising strategy to elicit breadth across emerging sarbecoviruses and represents an adaptable design approach for targeting conserved epitopes on other viral glycoproteins.
One Sentence Summary: SARS-CoV-2 immune focusing with engineered immunogens. |
| التعليقات: |
Update in: Cell Rep. 2022 Mar 22;38(12):110561. (PMID: 35303475) |
| معلومات مُعتمدة: |
DP2 DA040254 United States DA NIDA NIH HHS; F30 AI160908 United States AI NIAID NIH HHS; R01 AI146779 United States AI NIAID NIH HHS |
| تواريخ الأحداث: |
Date Created: 20210324 Latest Revision: 20230110 |
| رمز التحديث: |
20230110 |
| مُعرف محوري في PubMed: |
PMC7987010 |
| DOI: |
10.1101/2021.03.15.435440 |
| PMID: |
33758851 |
| قاعدة البيانات: |
MEDLINE |
