دورية أكاديمية
Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats.
| العنوان: | Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats. |
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| المؤلفون: | Salles BCC; Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, Brazil., Leme KC; Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, Brazil., da Silva MA; Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, Brazil., da Rocha CQ; Department of Chemistry, Federal University of Maranhão, São Luis, Brazil., Tangerina MMP; Institute of Biosciences, Coastal Campus of São Vicente, Universidade Estadual Paulista, São Vicente, Brazil.; Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo, Brazil., Vilegas W; Institute of Biosciences, Coastal Campus of São Vicente, Universidade Estadual Paulista, São Vicente, Brazil., Figueiredo SA; Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, Brazil., Duarte SMDS; Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, Brazil., Rodrigues MR; Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, Brazil., de Araújo Paula FB; Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, Federal University of Alfenas, Alfenas, Brazil. |
| المصدر: | The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2021 Sep 07; Vol. 73 (10), pp. 1361-1368. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0376363 Publication Model: Print Cited Medium: Internet ISSN: 2042-7158 (Electronic) Linking ISSN: 00223573 NLM ISO Abbreviation: J Pharm Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [Oxford] : Oxford University Press Original Publication: London : Pharmaceutical Society of Great Britain |
| مواضيع طبية MeSH: | Phytotherapy*, Diabetes Complications/*prevention & control , Diabetes Mellitus, Experimental/*drug therapy , Flavonoids/*therapeutic use , Glycosides/*therapeutic use , Passiflora/*chemistry , Plant Extracts/*therapeutic use, Animals ; Antioxidants/pharmacology ; Antioxidants/therapeutic use ; Apigenin/analysis ; Apigenin/pharmacology ; Apigenin/therapeutic use ; Blood Glucose/metabolism ; Chromatography, High Pressure Liquid ; Diabetes Complications/blood ; Diabetes Mellitus, Experimental/blood ; Diabetes Mellitus, Experimental/complications ; Flavones/analysis ; Flavones/pharmacology ; Flavones/therapeutic use ; Flavonoids/analysis ; Flavonoids/pharmacology ; Fructosamine/blood ; Glucosides/analysis ; Glucosides/pharmacology ; Glucosides/therapeutic use ; Glycosides/analysis ; Glycosides/pharmacology ; Luteolin/analysis ; Luteolin/pharmacology ; Luteolin/therapeutic use ; Male ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Plant Leaves/chemistry ; Platelet Aggregation/drug effects ; Rats, Wistar ; Tandem Mass Spectrometry ; Rats |
| مستخلص: | Objectives: This study was carried out to evaluate the effects of flavonoids present in leaves of Passiflora edulis fruit on complications induced by diabetes in rats. Methods: The extract of P. edulis leaf was obtained by 70% ethanol maceration. From the dry extract, the fractions were obtained by consecutive liquid-liquid partition with hexane, ethyl acetate and n-butanol. The content of isoorientin of ethyl acetate and n-butanol fractions was determined by ultra-performance liquid chromatography coupled with electrospray and triple quadrupole ionization (TQD) analysis in tandem mass spectrometry (UPLC-ESI-Tq-MS). Only Fr-BuOH was used to treat diabetic or not Wistar rats. Biochemical parameters, platelet aggregation and production of reactive species were evaluated. Key Findings: The UPLC-ESI-Tq-MS analysis revealed the presence of several flavonoids, among which we identified five possible flavonoids c-heterosides (luteolin-7-O-pyranosyl-3-O-glucoside, apigenin-6-8-di-C-glycoside, apigenin-6-C-arabinoside-8-C-glycoside, isoorientin, isovitexin). The diabetic rats (treated intraperitoneally with alloxan, 150 mg/kg) treated with Fr-BuOH (20 mg/kg/day for 90 days) presented improvement in blood glucose, serum levels of fructosamine, lipid profile and urea. Furthermore, the Fr-BuOH reduced both platelet aggregation and the production of oxidant species in diabetic animals. Conclusions: These results suggested that flavonoid C-glycosides present in the Fr-BuOH may be beneficial for the diabetic state, preventing complications induced by diabetes. (© The Author(s) 2021. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.) |
| معلومات مُعتمدة: | Coordination for the Improvement of Higher Education Personnel - Brazil |
| فهرسة مساهمة: | Keywords: Passiflora edulis; LC-MS quantification; antiplatelet; diabetes; isoorientin |
| المشرفين على المادة: | 0 (Antioxidants) 0 (Blood Glucose) 0 (Flavones) 0 (Flavonoids) 0 (Glucosides) 0 (Glycosides) 0 (Plant Extracts) 0 (violarvensin) 4429-04-3 (Fructosamine) 7V515PI7F6 (Apigenin) 98J6XDS46I (luteolin-7-glucoside) A37342TIX1 (homoorientin) KTQ9R9MS0Q (isovitexin) KUX1ZNC9J2 (Luteolin) |
| تواريخ الأحداث: | Date Created: 20210327 Date Completed: 20220117 Latest Revision: 20240226 |
| رمز التحديث: | 20240226 |
| DOI: | 10.1093/jpp/rgab046 |
| PMID: | 33772554 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2042-7158 |
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| DOI: | 10.1093/jpp/rgab046 |