دورية أكاديمية
The Distribution of Onion Virulence Gene Clusters Among Pantoea spp.
| العنوان: | The Distribution of Onion Virulence Gene Clusters Among Pantoea spp. |
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| المؤلفون: | Stice SP; Department of Plant Pathology, University of Georgia College of Agricultural and Environmental Sciences, Athens, GA, United States., Shin GY; Centre for Microbial Ecology and Genomics, Forestry and Agriculture Biotechnology Institute, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria, South Africa., De Armas S; Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay., Koirala S; Department of Plant Pathology, University of Georgia College of Agricultural and Environmental Sciences, Athens, GA, United States., Galván GA; Departamento de Producción Vegetal, Centro Regional Sur (CRS), Facultad de Agronomía, Universidad de la República, Canelones, Uruguay., Siri MI; Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay., Severns PM; Department of Plant Pathology, University of Georgia College of Agricultural and Environmental Sciences, Athens, GA, United States., Coutinho T; Centre for Microbial Ecology and Genomics, Forestry and Agriculture Biotechnology Institute, Department of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria, South Africa., Dutta B; Department of Plant Pathology, University of Georgia College of Agricultural and Environmental Sciences, Athens, GA, United States., Kvitko BH; Department of Plant Pathology, University of Georgia College of Agricultural and Environmental Sciences, Athens, GA, United States. |
| المصدر: | Frontiers in plant science [Front Plant Sci] 2021 Mar 10; Vol. 12, pp. 643787. Date of Electronic Publication: 2021 Mar 10 (Print Publication: 2021). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101568200 Publication Model: eCollection Cited Medium: Print ISSN: 1664-462X (Print) Linking ISSN: 1664462X NLM ISO Abbreviation: Front Plant Sci Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation, 2010- |
| مستخلص: | Pantoea ananatis is a gram-negative bacterium and the primary causal agent of center rot of onions in Georgia. Previous genomic studies identified two virulence gene clusters, HiVir and alt , associated with center rot. The HiVir gene cluster is required to induce necrosis on onion tissues via synthesis of pantaphos, (2-hydroxy[phosphono-methyl)maleate), a phosphonate phytotoxin. The alt gene cluster aids in tolerance to thiosulfinates generated during onion tissue damage. Whole genome sequencing of other Pantoea species suggests that these gene clusters are present outside of P. ananatis . To assess the distribution of these gene clusters, two PCR primer sets were designed to detect the presence of HiVir and alt . Two hundred fifty-two strains of Pantoea spp. were phenotyped using the red onion scale necrosis (RSN) assay and were genotyped using PCR for the presence of these virulence genes. A diverse panel of strains from three distinct culture collections comprised of 24 Pantoea species, 41 isolation sources, and 23 countries, collected from 1946-2019, was tested. There is a significant association between the alt PCR assay and Pantoea strains recovered from symptomatic onion ( P < 0.001). There is also a significant association of a positive HiVir PCR and RSN assay among P. ananatis strains but not among Pantoea spp., congeners. This may indicate a divergent HiVir cluster or different pathogenicity and virulence mechanisms. Last, we describe natural alt positive [RSN + /HiVir + / alt + ] P. ananatis strains, which cause extensive bulb necrosis in a neck-to-bulb infection assay compared to alt negative [RSN + /HiVir + / alt - ] P. ananatis strains. A combination of assays that include PCR of virulence genes [HiVir and alt ] and an RSN assay can potentially aid in identification of onion-bulb-rotting pathogenic P. ananatis strains. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Stice, Shin, De Armas, Koirala, Galván, Siri, Severns, Coutinho, Dutta and Kvitko.) |
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| فهرسة مساهمة: | Keywords: Pantoea ananatis; Pantoea spp.; bulb rotting; gene cluster distribution; onion; virulence gene |
| تواريخ الأحداث: | Date Created: 20210329 Latest Revision: 20231104 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7988079 |
| DOI: | 10.3389/fpls.2021.643787 |
| PMID: | 33777079 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-462X |
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| DOI: | 10.3389/fpls.2021.643787 |