دورية أكاديمية
أعرض في EDS

The LINC complex transmits integrin-dependent tension to the nuclear lamina and represses epidermal differentiation.

التفاصيل البيبلوغرافية
العنوان: The LINC complex transmits integrin-dependent tension to the nuclear lamina and represses epidermal differentiation.
المؤلفون: Carley E; Department of Cell Biology, Yale School of Medicine, New Haven, United States., Stewart RM; Department of Cell Biology, Yale School of Medicine, New Haven, United States., Zieman A; Department of Molecular, Cell and Developmental Biology, Yale University, New Haven, United States., Jalilian I; Department of Cell Biology, Yale School of Medicine, New Haven, United States., King DE; Sunnycrest Bioinformatics, Flemington, United States., Zubek A; Department of Dermatology, Yale School of Medicine, New Haven, United States., Lin S; Department of Molecular, Cell and Developmental Biology, Yale University, New Haven, United States., Horsley V; Department of Molecular, Cell and Developmental Biology, Yale University, New Haven, United States.; Department of Dermatology, Yale School of Medicine, New Haven, United States., King MC; Department of Cell Biology, Yale School of Medicine, New Haven, United States.; Department of Molecular, Cell and Developmental Biology, Yale University, New Haven, United States.
المصدر: ELife [Elife] 2021 Mar 29; Vol. 10. Date of Electronic Publication: 2021 Mar 29.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012-
مواضيع طبية MeSH: Epidermis/*physiology , Mechanotransduction, Cellular/*physiology , Nuclear Lamina/*physiology , Plakins/*genetics, Animals ; Cell Differentiation ; Female ; Integrins/metabolism ; Lamin Type A/metabolism ; Mice ; Plakins/metabolism
مستخلص: While the mechanisms by which chemical signals control cell fate have been well studied, the impact of mechanical inputs on cell fate decisions is not well understood. Here, using the well-defined system of keratinocyte differentiation in the skin, we examine whether and how direct force transmission to the nucleus regulates epidermal cell fate. Using a molecular biosensor, we find that tension on the nucleus through linker of nucleoskeleton and cytoskeleton (LINC) complexes requires integrin engagement in undifferentiated epidermal stem cells and is released during differentiation concomitant with decreased tension on A-type lamins. LINC complex ablation in mice reveals that LINC complexes are required to repress epidermal differentiation in vivo and in vitro and influence accessibility of epidermal differentiation genes, suggesting that force transduction from engaged integrins to the nucleus plays a role in maintaining keratinocyte progenitors. This work reveals a direct mechanotransduction pathway capable of relaying adhesion-specific signals to regulate cell fate.
Competing Interests: EC, RS, AZ, IJ, DK, AZ, SL No competing interests declared, VH, MK Reviewing editor, eLife
(© 2021, Carley et al.)
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معلومات مُعتمدة: R01 AR069550 United States NH NIH HHS; T32 GM007223 United States GM NIGMS NIH HHS; T32 AR007016 United States AR NIAMS NIH HHS; R01 AR060295 United States NH NIH HHS; T32 AR007016 United States NH NIH HHS; T32 GM007223 United States NH NIH HHS; R01 AR069550 United States AR NIAMS NIH HHS; R01 GM129308 United States GM NIGMS NIH HHS; R01 GM129308 United States NH NIH HHS; R01 AR060295 United States AR NIAMS NIH HHS
فهرسة مساهمة: Keywords: LINC complex; cell biology; differentiation; epidermis; mechanotransduction; mouse; nuclear lamina; regenerative medicine; stem cell; stem cells
المشرفين على المادة: 0 (Integrins)
0 (Lamin Type A)
0 (Plakins)
تواريخ الأحداث: Date Created: 20210329 Date Completed: 20210913 Latest Revision: 20240121
رمز التحديث: 20240121
مُعرف محوري في PubMed: PMC8051949
DOI: 10.7554/eLife.58541
PMID: 33779546
قاعدة البيانات: MEDLINE
الوصف
تدمد:2050-084X
DOI:10.7554/eLife.58541