دورية أكاديمية
أعرض في EDS

Core-shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor.

التفاصيل البيبلوغرافية
العنوان: Core-shell hydrogel microcapsules enable formation of human pluripotent stem cell spheroids and their cultivation in a stirred bioreactor.
المؤلفون: Fattahi P; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55902, USA., Rahimian A; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55902, USA., Slama MQ; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55902, USA., Gwon K; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55902, USA., Gonzalez-Suarez AM; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55902, USA., Wolf J; Department of Chemical and Biomolecular Engineering, Case Western Reserve University, Cleveland, OH, 44106, USA., Baskaran H; Department of Chemical and Biomolecular Engineering, Case Western Reserve University, Cleveland, OH, 44106, USA., Duffy CD; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55902, USA., Stybayeva G; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55902, USA., Peterson QP; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55902, USA., Revzin A; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55902, USA. revzin.alexander@mayo.edu.
المصدر: Scientific reports [Sci Rep] 2021 Mar 30; Vol. 11 (1), pp. 7177. Date of Electronic Publication: 2021 Mar 30.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Cell Culture Techniques/*methods , Pluripotent Stem Cells/*physiology , Spheroids, Cellular/*physiology, Bioreactors ; Capsules/chemistry ; Cell Culture Techniques/instrumentation ; Cell Differentiation ; Cell Line ; Cell Survival ; Cell Transplantation/methods ; Diabetes Mellitus/therapy ; End Stage Liver Disease/therapy ; Humans ; Hydrogels/chemistry ; Insulin-Secreting Cells/physiology ; Microfluidic Analytical Techniques/instrumentation ; Pluripotent Stem Cells/transplantation ; Polyethylene Glycols/chemistry
مستخلص: Cellular therapies based on human pluripotent stem cells (hPSCs) offer considerable promise for treating numerous diseases including diabetes and end stage liver failure. Stem cell spheroids may be cultured in stirred bioreactors to scale up cell production to cell numbers relevant for use in humans. Despite significant progress in bioreactor culture of stem cells, areas for improvement remain. In this study, we demonstrate that microfluidic encapsulation of hPSCs and formation of spheroids. A co-axial droplet microfluidic device was used to fabricate 400 μm diameter capsules with a poly(ethylene glycol) hydrogel shell and an aqueous core. Spheroid formation was demonstrated for three hPSC lines to highlight broad utility of this encapsulation technology. In-capsule differentiation of stem cell spheroids into pancreatic β-cells in suspension culture was also demonstrated.
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معلومات مُعتمدة: P41 EB021911 United States EB NIBIB NIH HHS
المشرفين على المادة: 0 (Capsules)
0 (Hydrogels)
3WJQ0SDW1A (Polyethylene Glycols)
تواريخ الأحداث: Date Created: 20210331 Date Completed: 20211025 Latest Revision: 20211025
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8010084
DOI: 10.1038/s41598-021-85786-2
PMID: 33785778
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-021-85786-2