التفاصيل البيبلوغرافية
| العنوان: |
Toward Community Surveillance: Detecting Intact SARS-CoV-2 Using Exogeneous Oligonucleotide Labels. |
| المؤلفون: |
Carey TR, Kozminsky M, Hall J, Vargas-Zapata V, Geiger K, Coscoy L, Sohn LL |
| المصدر: |
MedRxiv : the preprint server for health sciences [medRxiv] 2021 Mar 26. Date of Electronic Publication: 2021 Mar 26. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101767986 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: medRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
The persistence of the COVID-19 pandemic demands a dramatic increase in testing efficiency. Testing pooled samples for SARS-CoV-2 could meet this need; however, the sensitivity of RT-qPCR, the gold standard, significantly decreases with an increasing number of samples pooled. Here, we introduce DIVER, a method that quantifies intact virus and is robust to sample dilution. DIVER first tags viral particles with exogeneous oligonucleotides, then captures the tagged particles on ACE2-functionalized beads, and finally quantifies the oligonucleotide tags using qPCR. Using spike-presenting liposomes and Spike-pseudotyped lentivirus as SARS-CoV-2 models, we show that DIVER can detect 1×10 5 liposomes and 100 pfu lentivirus and can successfully identify positive samples in pooling experiments. Overall, DIVER is well-positioned for efficient sample pooling and expanded community surveillance. |
| التعليقات: |
Update in: Anal Chem. 2022 May 31;94(21):7619-7627. (PMID: 35584293) |
| معلومات مُعتمدة: |
F32 CA243354 United States CA NCI NIH HHS |
| تواريخ الأحداث: |
Date Created: 20210401 Latest Revision: 20221103 |
| رمز التحديث: |
20231215 |
| مُعرف محوري في PubMed: |
PMC8010747 |
| DOI: |
10.1101/2021.03.23.21254201 |
| PMID: |
33791715 |
| قاعدة البيانات: |
MEDLINE |
