دورية أكاديمية
Nanohybrid composed of graphene oxide functionalized with sodium hyaluronate accelerates bone healing in the tibia of rats.
| العنوان: | Nanohybrid composed of graphene oxide functionalized with sodium hyaluronate accelerates bone healing in the tibia of rats. |
|---|---|
| المؤلفون: | Dantas PCL; Faculty of Dentistry, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Rua da Glória 187, Diamantina, MG, Brazil., Martins-Júnior PA; Faculty of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil. Electronic address: pauloa-martinsjunior@ufmg.br., Coutinho DCO; Biological Sciences Institute, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil., Andrade VB; Biological Sciences Institute, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil., Valverde TM; Biological Sciences Institute, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil., Ávila ES; Institute of Exact Sciences, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil., Almeida TCS; Nuclear Technology Development Center, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil., Queiroz-Junior CM; Biological Sciences Institute, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil., Sá MA; Biological Sciences Institute, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil., Góes AM; Biological Sciences Institute, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil., Ladeira LO; Institute of Exact Sciences, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil., Ferreira AJ; Biological Sciences Institute, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, Brazil., Marques LS; Faculty of Dentistry, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Rua da Glória 187, Diamantina, MG, Brazil. |
| المصدر: | Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2021 Apr; Vol. 123, pp. 111961. Date of Electronic Publication: 2021 Feb 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101484109 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-0191 (Electronic) Linking ISSN: 09284931 NLM ISO Abbreviation: Mater Sci Eng C Mater Biol Appl Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Graphite*/pharmacology , Hyaluronic Acid*/pharmacology, Animals ; Rats ; Rats, Wistar ; Tibia ; Vascular Endothelial Growth Factor A |
| مستخلص: | This study synthesized and characterized a nanohybrid composed of graphene oxide (GO) functionalized with sodium hyaluronate (HY) (GO-HY), evaluated its effect in vitro and determined its osteogenic potential in vivo. The synthesized nanohybrid was analyzed by Scanning electron microscopy (SEM), Raman spectrometry, Thermogravimetry, Fourier-transform infrared (FTIR) spectroscopy and X-ray diffraction. MC3T3-E1 cell viability was assessed by MTT assay in 48 and 72 h. Bone defects were created in tibia of 40 Wistar rats and filled with blood clot (control), 1% HY, GO (50, 100 and 200 μg/mL) and the nanohybrid (50, 100 and 200 μg/mL). After 7 and 14 days, histomorphometric analysis was carried out to assess osteogenic potential of the nanohybrid. Immunohistochemical analysis evaluated the expression of vascular endothelial growth factor (VEGF) in bone defects. Thermogravimetric analysis, Raman and FTIR spectrometry confirmed the functionalization of GO with HY by covalent bonds. Five μg/mL concentrations of the nanohybrid did not alter the viability of the MC3T3-E1 cells. Histomorphometric analysis demonstrated that the nanohybrid at 100 μg/mL significantly accelerated the bone repair in tibia of rats when compared to controls (p < 0.01). Immunohistochemical analysis showed a significantly less intense VEGF expression in tibia treated with the nanohybrid when compared to controls (p < 0.05). The nanohybrid composed of GO functionalized with HY was able to induce the acceleration of the tissue regeneration process in bone defects created in the tibia of rats. This novel nanohybrid is a promising material for the field of bone tissue engineering. (Copyright © 2021 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bone repair; Bone tissue engineering; Graphene; Nanomaterial; Sodium hyaluronate |
| المشرفين على المادة: | 0 (Vascular Endothelial Growth Factor A) 0 (graphene oxide) 7782-42-5 (Graphite) 9004-61-9 (Hyaluronic Acid) |
| تواريخ الأحداث: | Date Created: 20210404 Date Completed: 20210514 Latest Revision: 20210514 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.msec.2021.111961 |
| PMID: | 33812589 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder كن أول من يترك تعليقا!