دورية أكاديمية
Comprehensive Analysis of DNA Adducts Using Data-Independent wSIM/MS 2 Acquisition and wSIM-City.
| العنوان: | Comprehensive Analysis of DNA Adducts Using Data-Independent wSIM/MS 2 Acquisition and wSIM-City. |
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| المؤلفون: | Walmsley SJ; Masonic Cancer Center, University of Minnesota, Minneapolis 55455, Minnesota, United States.; Institute of Health Informatics, University of Minnesota, Minneapolis 55455, Minnesota, United States., Guo J; Masonic Cancer Center, University of Minnesota, Minneapolis 55455, Minnesota, United States.; Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis 55455, Minnesota, United States., Murugan P; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455, Minnesota, United States., Weight CJ; Glickman Urologic and Kidney Institute, Cleveland Clinic, Cleveland 44125, Ohio, United States.; Case Comprehensive Cancer Center, Cleveland 44106, Ohio, United States., Wang J; Masonic Cancer Center, University of Minnesota, Minneapolis 55455, Minnesota, United States.; Institute of Health Informatics, University of Minnesota, Minneapolis 55455, Minnesota, United States., Villalta PW; Masonic Cancer Center, University of Minnesota, Minneapolis 55455, Minnesota, United States.; Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis 55455, Minnesota, United States., Turesky RJ; Masonic Cancer Center, University of Minnesota, Minneapolis 55455, Minnesota, United States.; Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis 55455, Minnesota, United States. |
| المصدر: | Analytical chemistry [Anal Chem] 2021 Apr 27; Vol. 93 (16), pp. 6491-6500. Date of Electronic Publication: 2021 Apr 12. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
| مواضيع طبية MeSH: | DNA* , DNA Adducts* , Spectrometry, Mass, Electrospray Ionization*, Humans ; Mass Spectrometry ; Nucleotides ; Xenobiotics |
| مستخلص: | A novel software has been created to comprehensively characterize covalent modifications of DNA through mass spectral analysis of enzymatically hydrolyzed DNA using the neutral loss of 2'-deoxyribose, a nearly universal MS 2 fragmentation process of protonated 2'-deoxyribonucleosides. These covalent modifications termed DNA adducts form through xenobiotic exposures or by reaction with endogenous electrophiles and can induce mutations during cell division and initiate carcinogenesis. DNA adducts are typically present at trace levels in the human genome, requiring a very sensitive and comprehensive data acquisition and analysis method. Our software, wSIM-City, was created to process mass spectral data acquired by a wide selected ion monitoring (wSIM) with gas-phase fractionation and coupled to wide MS 2 fragmentation. This untargeted approach can detect DNA adducts at trace levels as low as 1.5 adducts per 10 9 nucleotides. This level of sensitivity is sufficient for comprehensive analysis and characterization of DNA modifications in human specimens. |
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| معلومات مُعتمدة: | U2C ES026533 United States ES NIEHS NIH HHS; R03 ES031188 United States ES NIEHS NIH HHS; P30 CA077598 United States CA NCI NIH HHS; R50 CA211256 United States CA NCI NIH HHS; UL1 TR000114 United States TR NCATS NIH HHS; R01 CA122320 United States CA NCI NIH HHS; R01 ES030765 United States ES NIEHS NIH HHS; R01 ES019564 United States ES NIEHS NIH HHS; R01 CA220367 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (DNA Adducts) 0 (Nucleotides) 0 (Xenobiotics) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20210412 Date Completed: 20210525 Latest Revision: 20230921 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8675643 |
| DOI: | 10.1021/acs.analchem.1c00362 |
| PMID: | 33844920 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-6882 |
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| DOI: | 10.1021/acs.analchem.1c00362 |