دورية أكاديمية
أعرض في EDS

Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy.

التفاصيل البيبلوغرافية
العنوان: Label-Free Evaluation of Chromatin Condensation in Human Normal Morphology Sperm Using Raman Spectroscopy.
المؤلفون: Jahmani MY; Department of Obstetrics and Gynecology, Assisted Reproductive Laboratory, Saarland University Hospital, Homburg, Germany., Hammadeh ME; Department of Obstetrics and Gynecology, Assisted Reproductive Laboratory, Saarland University Hospital, Homburg, Germany., Al Smadi MA; Department of Obstetrics and Gynecology, Assisted Reproductive Laboratory, Saarland University Hospital, Homburg, Germany., Baller MK; Department of Informatics and Microsystems Technology, University of Applied Sciences Kaiserslautern, Campus, Zweibrücken, Germany. marko.baller@hs-kl.de.
المصدر: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2021 Sep; Vol. 28 (9), pp. 2527-2539. Date of Electronic Publication: 2021 Apr 20.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 101291249 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1933-7205 (Electronic) Linking ISSN: 19337191 NLM ISO Abbreviation: Reprod Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: 2020- : [New York] : Springer
Original Publication: Thousand Oaks, Calif. : Sage
مواضيع طبية MeSH: Chromatin Assembly and Disassembly* , DNA Damage* , Microscopy, Fluorescence* , Semen Analysis* , Spectrum Analysis, Raman*, Chromatin/*chemistry , Spermatozoa/*chemistry, Adult ; Chromatin/pathology ; Humans ; Male ; Predictive Value of Tests ; Spermatozoa/pathology ; Staining and Labeling
مستخلص: Chromatin condensation is one of the main factors essential for sperm function. Evaluation of chromatin condensation by current methods render the assessed sperm unsuitable for assisted reproduction. We examined the Raman spectra of normal morphology sperm to determine whether a non-invasive confocal Raman spectroscopy can detect spectral differences between groups having different levels of chromatin condensation. Semen samples from 85 donors who underwent ICSI were obtained. Chromomycin A3, aniline blue and acridine orange staining were performed to evaluate the protamine deficiency, histone retention and DNA fragmentation respectively. Raman spectra were obtained from 50 normal morphology sperm for each donor. Spectral analysis was performed using home written programs in LabVIEW software and samples were grouped based on chromomycin A3 staining. Raman peaks intensities at 670 cm -1 , 731 cm -1 , 785 cm -1 , 858 cm -1 , 1062 cm -1 , 1098 cm -1 , 1185 cm -1 , 1372 cm -1 , 1424 cm -1 , 1450 cm -1 , 1532 cm -1 , 1618 cm -1 and 1673 cm -1 were significantly correlated with at least one of the sperm staining methods. The median intensity of the Raman peaks at 670 cm -1 , 731 cm -1 , 785 cm -1 , 1062 cm -1 , 1098 cm -1 , 1185 cm -1 , 1372 cm -1 , 1424 cm -1 , 1450 cm -1 , 1532 cm -1 , 1618 cm -1 and 1673 cm -1 show a significant difference between the CMA3≤41 and CMA3>41groups. The Raman spectroscopic measurements represent a promising diagnostic tool that has the ability to label-free detect sperm with chromatin abnormalities, such as improper chromatin condensation and DNA fragmentation to a certain degree similar to that of the existing staining techniques at the individual cell level.
(© 2021. The Author(s).)
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فهرسة مساهمة: Keywords: Chromatin condensation; Chromomycin A3; Raman spectroscopy; Sperm
المشرفين على المادة: 0 (Chromatin)
تواريخ الأحداث: Date Created: 20210420 Date Completed: 20220125 Latest Revision: 20220218
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8346437
DOI: 10.1007/s43032-021-00494-6
PMID: 33877640
قاعدة البيانات: MEDLINE
الوصف
تدمد:1933-7205
DOI:10.1007/s43032-021-00494-6