دورية أكاديمية
Inhibition of Aedes aegypti DNA topoisomerase II by etoposide: Impact on survival and morphology of larvae and pupae.
| العنوان: | Inhibition of Aedes aegypti DNA topoisomerase II by etoposide: Impact on survival and morphology of larvae and pupae. |
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| المؤلفون: | Santos D; Laboratório Integrado de Bioquímica Hatisaburo Masuda, NUPEM, Instituto de Biodiversidade e Sustentabilidade, Universidade Federal do Rio de Janeiro, Avenida São José Barreto, N° 764, Bairro, São José do Barreto, Macaé, RJ CEP: 27.965-045, Brazil., Gomes HF; Laboratório Integrado de Bioquímica Hatisaburo Masuda, NUPEM, Instituto de Biodiversidade e Sustentabilidade, Universidade Federal do Rio de Janeiro, Avenida São José Barreto, N° 764, Bairro, São José do Barreto, Macaé, RJ CEP: 27.965-045, Brazil., Ribeiro L; Laboratório Integrado Ciências Morfofuncionais, NUPEM, Instituto de Biodiversidade e Sustentabilidade, Universidade Federal do Rio de Janeiro, Avenida São José Barreto, N° 764, Bairro, São José do Barreto, Macaé, RJ CEP: 27.965-045, Brazil., Farias AB; Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro CEP: 21941-909, Brazil; Laboratório Integrado de Computação Científica, Universidade Federal do Rio de Janeiro, Campus Macaé Professor Aloísio Teixeira, Avenida Aluízio da Silva Gomes, 50, CEP 27930-560, Granja dos Cavaleiros, Macaé, RJ, Brazil., Romeiro NC; Laboratório Integrado de Computação Científica, Universidade Federal do Rio de Janeiro, Campus Macaé Professor Aloísio Teixeira, Avenida Aluízio da Silva Gomes, 50, CEP 27930-560, Granja dos Cavaleiros, Macaé, RJ, Brazil., da Fonseca RN; Laboratório Integrado Ciências Morfofuncionais, NUPEM, Instituto de Biodiversidade e Sustentabilidade, Universidade Federal do Rio de Janeiro, Avenida São José Barreto, N° 764, Bairro, São José do Barreto, Macaé, RJ CEP: 27.965-045, Brazil., Nepomuceno-Silva JL; Laboratório Integrado de Bioquímica Hatisaburo Masuda, NUPEM, Instituto de Biodiversidade e Sustentabilidade, Universidade Federal do Rio de Janeiro, Avenida São José Barreto, N° 764, Bairro, São José do Barreto, Macaé, RJ CEP: 27.965-045, Brazil., Moraes J; Laboratório Integrado de Bioquímica Hatisaburo Masuda, NUPEM, Instituto de Biodiversidade e Sustentabilidade, Universidade Federal do Rio de Janeiro, Avenida São José Barreto, N° 764, Bairro, São José do Barreto, Macaé, RJ CEP: 27.965-045, Brazil. Electronic address: jorgemoraes@bioqmed.ufrj.br. |
| المصدر: | Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2021 Sep; Vol. 247, pp. 109066. Date of Electronic Publication: 2021 Apr 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: United States NLM ID: 100959500 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-0456 (Print) Linking ISSN: 15320456 NLM ISO Abbreviation: Comp Biochem Physiol C Toxicol Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Elsevier Science, c2000- |
| مواضيع طبية MeSH: | Aedes*/enzymology , Aedes*/growth & development, DNA Topoisomerases, Type II/*metabolism , Etoposide/*toxicity , Larva/*drug effects , Mosquito Vectors/*drug effects , Topoisomerase II Inhibitors/*toxicity, Animals |
| مستخلص: | DNA topoisomerase II enzymes maintain DNA stability during vital processes, such as genome replication, transcription and chromosomal segregation during mitosis and meiosis. In the present work, we analyzed functional aspects of the DNA topoisomerase II (AeTopII) enzyme of the mosquito Aedes aegypti. Here, we show that AeTopII mRNA is expressed at all stages of mosquito development. By in situ hybridization, we found that the AeTopII mRNA is concentrated along the ovarian follicular cells as well as in the region of the follicles. The observed expression profiles likely reflect increased topoisomerase II cellular requirements due to the intense ovarian growth and egg production following blood feeding in Ae. aegypti females. The drug etoposide, a classic inhibitor of topoisomerase II, was used for in vivo testing with 2nd stage larvae, in order to investigate the functional importance of this enzyme in Ae. aegypti survival and development. Inhibition of topoisomerase II activity with etoposide concentrations ranging from 10 to 200 μM did not leads to the immediate death of larvae. However, after 10 days of observation, etoposide treatments resulted in 30-40% decrease in survival, in a dose dependent manner, with persisting larvae and pupae presenting incomplete development, as well as morphological abnormalities. Also, approximately 50% of the treated larvae did not reach the pupal stage. Thus, we conclude that AeTopII is a vital enzyme in the development of Ae. aegypti and its sensitivity to inhibitors should be explored for potential chemical agents to be used in vector control. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aedes aegypti; DNA topoisomerase II; Etoposide; Mosquito |
| المشرفين على المادة: | 0 (Topoisomerase II Inhibitors) 6PLQ3CP4P3 (Etoposide) EC 5.99.1.3 (DNA Topoisomerases, Type II) |
| تواريخ الأحداث: | Date Created: 20210430 Date Completed: 20211213 Latest Revision: 20211214 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.cbpc.2021.109066 |
| PMID: | 33930525 |
| قاعدة البيانات: | MEDLINE |
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