High Proportion of Genome-Wide Homology and Increased Pretreatment pvcrt Levels in Plasmodium vivax Late Recurrences: a Chloroquine Therapeutic Efficacy Study.

التفاصيل البيبلوغرافية
العنوان:	High Proportion of Genome-Wide Homology and Increased Pretreatment pvcrt Levels in Plasmodium vivax Late Recurrences: a Chloroquine Therapeutic Efficacy Study.
المؤلفون:	Rovira-Vallbona E; Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium., Van Hong N; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Kattenberg JH; Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium., Huan RM; Centre for Disease Control and Prevention, Pleiku, Gia Lai, Vietnam., Binh NTH; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Ngọc NTH; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Guetens P; Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium., Hieu NL; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Hien NTT; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Sang VT; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Long ND; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Sauve E; Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium., Duong TT; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Xa NX; National Institute of Malariology, Parasitology and Entomology, Hanoi, Vietnam., Erhart A; Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.; Medical Research Council Unit The Gambia at the London School of Hygiene and Tropical Medicine, Fajara, The Gambia., Rosanas-Urgell A; Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
المصدر:	Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2021 Jul 16; Vol. 65 (8), pp. e0009521. Date of Electronic Publication: 2021 Jul 16.
نوع المنشور:	Journal Article; Research Support, Non-U.S. Gov't
اللغة:	English
بيانات الدورية:	Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 0315061 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-6596 (Electronic) Linking ISSN: 00664804 NLM ISO Abbreviation: Antimicrob Agents Chemother Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Washington, American Society for Microbiology
مواضيع طبية MeSH:	Antimalarials/pharmacology , Antimalarials/therapeutic use , Malaria, Vivax*/drug therapy, Adult ; Chloroquine/therapeutic use ; Drug Resistance/genetics ; Humans ; Plasmodium vivax/genetics ; Recurrence ; Young Adult
مستخلص:	Chloroquine (CQ) is the first-line treatment for Plasmodium vivax malaria in most countries where malaria is endemic. Monitoring P. vivax CQ resistance (CQR) is critical but remains challenged by the difficulty to distinguish real treatment failure from reinfection or liver relapse. The therapeutic efficacy of CQ against uncomplicated P. vivax malaria was evaluated in Gia Lai Province, Vietnam. Sixty-seven patients were enrolled and followed for 42 days using microscopy and quantitative PCR. Adequate clinical and parasitological response (ACPR) was 100% (66/66) on day 28 but 75.4% (49/65) on day 42. Eighteen recurrences (27.7%) were detected, with a median time to recurrence of 42 days (interquartile range [IQR], 35 to 42) and blood CQ concentration of <100 ng/ml. Primary infections leading to recurrence occurred in younger individuals (median age for ACPR = 25 years [IQR, 20 to 28]; recurrences = 18 [16 to 21]; P = 0.002) had a longer parasite clearance time (PCT for ACPR = 47.5 h [IQR, 36.2 to 59.8 h]; recurrences = 54.2 [48.4 to 62.0]; P = 0.035) and higher pvcrt gene expression (median relative expression ratio for ACPR = 0.09 [IQR, 0.05 to 0.22]; recurrences = 0.20 [0.15 to 0.56]; P = 0.002), but showed no differences in ex vivo CQ sensitivity. Parasite genotyping by microsatellites, single nucleotide polymorphism (SNP) barcoding, and whole-genome sequencing (WGS) identified a majority of homologous recurrences, with 80% (8/10) showing >98% identity by descent to paired day 0 samples. This study shows that CQ remained largely efficacious to treat P. vivax in Gia Lai; i.e., recurrences occurred late (>day 28) and in the presence of low blood CQ concentrations. However, the combination of both WGS and gene expression analysis ( pvcrt ) data with clinical data (PCT) allowed us to identify potential emergence of low-grade CQR, which should be closely monitored. (This study has been registered at ClinicalTrials.gov under identifier NCT02610686.).
References:	Nat Commun. 2019 Jan 22;10(1):371. (PMID: 30670687) Nat Commun. 2019 Dec 6;10(1):5595. (PMID: 31811128) Malar J. 2011 Oct 11;10:297. (PMID: 21989376) mBio. 2018 Jan 23;9(1):. (PMID: 29362233) Malar J. 2018 Apr 27;17(1):180. (PMID: 29703200) Malar J. 2010 Dec 14;9:361. (PMID: 21156052) Antimicrob Agents Chemother. 2013 Jul;57(7):3121-30. (PMID: 23612201) Trends Parasitol. 2020 Jun;36(6):560-570. (PMID: 32407682) J Antimicrob Chemother. 2020 Aug 1;75(8):2272-2281. (PMID: 32437557) PLoS One. 2014 Aug 26;9(8):e105922. (PMID: 25157811) PLoS One. 2007 Oct 31;2(10):e1089. (PMID: 17971853) PLoS Negl Trop Dis. 2016 Feb 12;10(2):e0004434. (PMID: 26872387) Blood. 2015 Feb 19;125(8):1203-5. (PMID: 25700424) Am J Trop Med Hyg. 2016 Apr;94(4):879-885. (PMID: 26856909) Am J Trop Med Hyg. 2013 Jun;88(6):1116-23. (PMID: 23509126) PLoS One. 2012;7(2):e32891. (PMID: 22393456) PLoS Negl Trop Dis. 2017 Apr 6;11(4):e0005365. (PMID: 28384192) Nat Genet. 2016 Aug;48(8):959-964. (PMID: 27348299) Malar J. 2019 Jan 17;18(1):10. (PMID: 30654808) J Infect Dis. 2005 Jan 15;191(2):272-7. (PMID: 15609238) Trends Parasitol. 2012 Nov;28(11):522-9. (PMID: 23044287) Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404) Am J Trop Med Hyg. 2010 Feb;82(2):223-7. (PMID: 20133996) Malar J. 2018 Jun 7;17(1):226. (PMID: 29880051) Parasitology. 2018 Nov;145(13):1765-1771. (PMID: 29564998) mBio. 2017 Feb 7;8(1):. (PMID: 28174312) Malar J. 2005 Feb 11;4:11. (PMID: 15707493) J Infect Dis. 2009 Apr 1;199(7):1074-80. (PMID: 19275476) J Infect Dis. 2019 Jan 7;219(2):315-322. (PMID: 30102351) Trends Parasitol. 2020 May;36(5):447-458. (PMID: 32298632) Antimicrob Agents Chemother. 2004 Nov;48(11):4075-83. (PMID: 15504824) Trends Parasitol. 2013 Jun;29(6):286-94. (PMID: 23623759) J Infect Dis. 2008 Nov 15;198(10):1558-64. (PMID: 18808339) Malar J. 2020 Jul 27;19(1):271. (PMID: 32718342) J Infect Dis. 2019 Oct 22;220(11):1738-1749. (PMID: 30668735) Malar J. 2011 Nov 10;10:339. (PMID: 22074219) Front Plant Sci. 2014 Apr 29;5:153. (PMID: 24808897) Nucleic Acids Res. 2001 May 1;29(9):e45. (PMID: 11328886) Antimicrob Agents Chemother. 2015 Nov 02;60(1):361-7. (PMID: 26525783) Antimicrob Agents Chemother. 2015 Dec;59(12):7411-9. (PMID: 26392501) PLoS One. 2013 Sep 27;8(9):e76316. (PMID: 24312682) Am J Trop Med Hyg. 2016 Dec 28;95(6 Suppl):15-34. (PMID: 27402513) Nat Commun. 2019 Sep 20;10(1):4300. (PMID: 31541097) Lancet Infect Dis. 2014 Oct;14(10):982-91. (PMID: 25213732) mBio. 2018 May 8;9(3):. (PMID: 29739900) Trop Med Int Health. 2002 Oct;7(10):858-64. (PMID: 12358621) PLoS Negl Trop Dis. 2015 Mar 17;9(3):e0003539. (PMID: 25781890) Malar J. 2015 Jul 08;14:264. (PMID: 26152336) Malar J. 2018 Jul 16;17(1):267. (PMID: 30012145) Antimicrob Agents Chemother. 2008 Dec;52(12):4233-40. (PMID: 18809933)
معلومات مُعتمدة:	United Kingdom WT_ Wellcome Trust
فهرسة مساهمة:	Keywords: Plasmodium vivax; Vietnam; antimalarial agents; chloroquine; drug resistance; malaria; pvcrt; recurrence; therapeutic efficacy; whole-genome sequencing
سلسلة جزيئية:	ClinicalTrials.gov NCT02610686
المشرفين على المادة:	0 (Antimalarials) 886U3H6UFF (Chloroquine)
تواريخ الأحداث:	Date Created: 20210525 Date Completed: 20210809 Latest Revision: 20220117
رمز التحديث:	20240829
مُعرف محوري في PubMed:	PMC8284456
DOI:	10.1128/AAC.00095-21
PMID:	34031050
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	1098-6596
DOI:	10.1128/AAC.00095-21