دورية أكاديمية
Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification.
| العنوان: | Development of Simple Analytical Method for B-Group Vitamins in Nutritional Products: Enzymatic Digestion and UPLC-MS/MS Quantification. |
|---|---|
| المؤلفون: | Nguyen QH; Faculty of Chemistry, University of Science, Vietnam National University, 19 Le Thanh Tong, Hanoi 10000, Vietnam.; Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Thai Nguyen University of Medicine and Pharmacy, Thai Nguyen 24000, Vietnam., Hoang AQ; Faculty of Chemistry, University of Science, Vietnam National University, 19 Le Thanh Tong, Hanoi 10000, Vietnam., Truong TMH; Faculty of Chemistry, University of Science, Vietnam National University, 19 Le Thanh Tong, Hanoi 10000, Vietnam., Dinh TD; Faculty of Chemistry, University of Science, Vietnam National University, 19 Le Thanh Tong, Hanoi 10000, Vietnam., Le TT; National Institute for Food Control (NIFC), 65 Pham Than Duat, Hanoi 10000, Vietnam., Luu THT; National Institute for Food Control (NIFC), 65 Pham Than Duat, Hanoi 10000, Vietnam., Dinh VC; National Institute for Food Control (NIFC), 65 Pham Than Duat, Hanoi 10000, Vietnam., Nguyen TMT; Faculty of Chemistry, University of Science, Vietnam National University, 19 Le Thanh Tong, Hanoi 10000, Vietnam., Vu TT; National Institute for Food Control (NIFC), 65 Pham Than Duat, Hanoi 10000, Vietnam., Nguyen TAH; Faculty of Chemistry, University of Science, Vietnam National University, 19 Le Thanh Tong, Hanoi 10000, Vietnam. |
| المصدر: | Journal of analytical methods in chemistry [J Anal Methods Chem] 2021 May 05; Vol. 2021, pp. 5526882. Date of Electronic Publication: 2021 May 05 (Print Publication: 2021). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Hindawi Pub. Corp Country of Publication: Egypt NLM ID: 101575377 Publication Model: eCollection Cited Medium: Print ISSN: 2090-8865 (Print) Linking ISSN: 20908873 NLM ISO Abbreviation: J Anal Methods Chem Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Cario : Hindawi Pub. Corp. |
| مستخلص: | A method for the simultaneous determination of seven B-group vitamers including thiamine, riboflavin, nicotinamide, niacin, pyridoxine, pyridoxal, and pyridoxamine in nutritional products by using enzymatic digestion followed by LC-MS/MS quantification was studied. The LC-MS/MS conditions such as MS transitions, mobile phase programs, and ammonium formate buffer concentrations, and sample treatment procedures (e.g., concentrations of buffer solution, digestion temperature, and digestion time) were investigated. The analytical method performance was evaluated by multiple criteria such as selectivity, linearity, detection and quantification limits, repeatability, reproducibility, and recovery by using real sample matrices. The validated method was successfully applied to analyze vitamin B concentrations in different nutritional products like ultra-heat-treated milk, powdered milk, and nutritional powder. Vitamin B concentrations varied over a wide range from lower than detection limits to about 9000 µ g/100 g, depending on vitamin groups, compound forms, and sample types. The measured concentrations of B-group vitamins in our samples were generally in good agreement with values of label claims. Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this paper. (Copyright © 2021 Quang Huy Nguyen et al.) |
| References: | Crit Rev Food Sci Nutr. 2009 Apr;49(4):361-8. (PMID: 19234945) Rapid Commun Mass Spectrom. 2008 Jul;22(13):2029-43. (PMID: 18512845) J Chromatogr A. 2003 Jul 25;1007(1-2):77-84. (PMID: 12924553) J Chromatogr A. 2000 Feb 18;870(1-2):207-15. (PMID: 10722078) J Chromatogr A. 1997 Aug 22;778(1-2):247-53. (PMID: 9299739) J AOAC Int. 2020 Jul 1;103(4):1060-1072. (PMID: 33241348) Can J Comp Med. 1984 Apr;48(2):175-8. (PMID: 6722645) J Chromatogr A. 2019 Nov 22;1606:360245. (PMID: 31122728) Molecules. 2020 Oct 06;25(19):. (PMID: 33036314) J Chromatogr A. 2020 Jan 4;1609:460452. (PMID: 31443970) ACS Appl Mater Interfaces. 2018 Jan 31;10(4):4140-4150. (PMID: 29299926) Braz J Microbiol. 2011 Oct;42(4):1397-404. (PMID: 24031769) Biomed Environ Sci. 2015 Oct;28(10):738-50. (PMID: 26582096) J Agric Food Chem. 2006 Jun 28;54(13):4531-6. (PMID: 16786994) Anal Bioanal Chem. 2020 Oct;412(26):7237-7252. (PMID: 32797304) |
| تواريخ الأحداث: | Date Created: 20210526 Latest Revision: 20210528 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8116160 |
| DOI: | 10.1155/2021/5526882 |
| PMID: | 34035973 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder كن أول من يترك تعليقا!