دورية أكاديمية
Copper binding by a unique family of metalloproteins is dependent on kynurenine formation.
العنوان: | Copper binding by a unique family of metalloproteins is dependent on kynurenine formation. |
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المؤلفون: | Manesis AC; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208.; Department of Chemistry, Northwestern University, Evanston, IL 60208., Jodts RJ; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208.; Department of Chemistry, Northwestern University, Evanston, IL 60208., Hoffman BM; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208.; Department of Chemistry, Northwestern University, Evanston, IL 60208., Rosenzweig AC; Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208; amyr@northwestern.edu.; Department of Chemistry, Northwestern University, Evanston, IL 60208. |
المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Jun 08; Vol. 118 (23). |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
اللغة: | English |
بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
مواضيع طبية MeSH: | Bacterial Proteins/*chemistry , Copper/*chemistry , Kynurenine/*chemistry , Metalloproteins/*chemistry , Methylosinus trichosporium/*chemistry, Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Copper/metabolism ; Crystallography, X-Ray ; Kynurenine/biosynthesis ; Kynurenine/genetics ; Metalloproteins/genetics ; Metalloproteins/metabolism ; Methylosinus trichosporium/genetics ; Methylosinus trichosporium/metabolism ; Protein Domains |
مستخلص: | Some methane-oxidizing bacteria use the ribosomally synthesized, posttranslationally modified natural product methanobactin (Mbn) to acquire copper for their primary metabolic enzyme, particulate methane monooxygenase. The operons encoding the machinery to biosynthesize and transport Mbns typically include genes for two proteins, MbnH and MbnP, which are also found as a pair in other genomic contexts related to copper homeostasis. While the MbnH protein, a member of the bacterial diheme cytochrome c peroxidase (bC c P)/MauG superfamily, has been characterized, the structure and function of MbnP, the relationship between the two proteins, and their role in copper homeostasis remain unclear. Biochemical characterization of MbnP from the methanotroph Methylosinus trichosporium OB3b now reveals that MbnP binds a single copper ion, present in the +1 oxidation state, with high affinity. Copper binding to MbnP in vivo is dependent on oxidation of the first tryptophan in a conserved WxW motif to a kynurenine, a transformation that occurs through an interaction of MbnH with MbnP. The 2.04-Å-resolution crystal structure of MbnP reveals a unique fold and an unusual copper-binding site involving a histidine, a methionine, a solvent ligand, and the kynurenine. Although the kynurenine residue may not serve as a Cu I primary-sphere ligand, being positioned ∼2.9 Å away from the Cu I ion, its presence is required for copper binding. Genomic neighborhood analysis indicates that MbnP proteins, and by extension kynurenine-containing copper sites, are widespread and may play diverse roles in microbial copper homeostasis. Competing Interests: The authors declare no competing interest. |
References: | J Biol Chem. 2018 Mar 30;293(13):4606-4615. (PMID: 29348173) J Inorg Biochem. 2012 May;110:72-82. (PMID: 22504273) Methods Mol Biol. 2014;1091:17-32. (PMID: 24203322) BMC Biol. 2013 Feb 26;11:17. (PMID: 23442874) Biochem Biophys Res Commun. 1998 Oct 29;251(3):744-7. (PMID: 9790980) FEMS Microbiol Lett. 1997 Mar 15;148(2):169-74. (PMID: 9084144) Biochemistry. 2017 Aug 22;56(33):4293-4308. (PMID: 28826221) Metallomics. 2017 Jan 25;9(1):7-20. (PMID: 27905614) J Bacteriol. 2003 Oct;185(19):5755-64. (PMID: 13129946) Biochemistry. 2018 Jun 26;57(25):3515-3523. (PMID: 29694778) Protein Sci. 2018 Jan;27(1):293-315. (PMID: 29067766) J Am Chem Soc. 2011 Mar 9;133(9):2983-8. (PMID: 21323310) Chemistry. 2018 Mar 26;24(18):4515-4518. (PMID: 29365216) J Biol Chem. 2019 Nov 1;294(44):16141-16151. (PMID: 31511324) Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):13027-13032. (PMID: 27807137) J Biol Chem. 2011 Apr 1;286(13):11047-55. (PMID: 21258123) Acta Crystallogr D Biol Crystallogr. 2009 Jun;65(Pt 6):582-601. (PMID: 19465773) Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):9639-44. (PMID: 23720312) Biochim Biophys Acta. 2015 Aug;1854(8):1019-37. (PMID: 25900361) Biochemistry. 2016 Apr 19;55(15):2278-90. (PMID: 27010565) Inorg Chem. 2011 Feb 21;50(4):1378-91. (PMID: 21254756) Curr Protoc Bioinformatics. 2013 Jun;Chapter 3:Unit3.1. (PMID: 23749753) Inorg Chem. 2001 May 7;40(10):2246-55. (PMID: 11327898) Dalton Trans. 2015 Oct 7;44(37):16494-505. (PMID: 26327397) Nat Commun. 2018 May 17;9(1):1981. (PMID: 29773791) Mol Biol Evol. 2013 Apr;30(4):772-80. (PMID: 23329690) Proc Natl Acad Sci U S A. 2011 Oct 11;108(41):16956-61. (PMID: 21969534) J Biol Chem. 2008 May 16;283(20):13897-904. (PMID: 18348978) J Biochem. 1982 Jul;92(1):129-41. (PMID: 7118867) Metallomics. 2016 Sep 1;8(9):931-40. (PMID: 27087171) Anal Biochem. 1976 May 7;72:248-54. (PMID: 942051) Anal Biochem. 2013 Dec 15;443(2):222-31. (PMID: 24036037) Mikrobiologiia. 2011 Sep-Oct;80(5):595-605. (PMID: 22168002) Nucleic Acids Res. 2011 Jul;39(Web Server issue):W29-37. (PMID: 21593126) Acc Chem Res. 2014 Dec 16;47(12):3605-13. (PMID: 25310275) Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):678-82. (PMID: 16699196) Biochemistry. 2015 Apr 14;54(14):2283-94. (PMID: 25806595) Science. 2018 Mar 23;359(6382):1411-1416. (PMID: 29567715) Annu Rev Biochem. 2018 Jun 20;87:645-676. (PMID: 29668305) Proteins. 2002 May 15;47(3):344-56. (PMID: 11948788) Annu Rev Biochem. 2019 Jun 20;88:409-431. (PMID: 30633550) Proc Natl Acad Sci U S A. 2011 Aug 9;108(32):13007-12. (PMID: 21778408) Nat Chem Biol. 2008 Oct;4(10):599-601. (PMID: 18758441) PLoS One. 2014 Feb 03;9(2):e87750. (PMID: 24498370) J Mol Biol. 2007 Sep 21;372(3):774-97. (PMID: 17681537) Nucleic Acids Res. 2018 Jul 2;46(W1):W200-W204. (PMID: 29905871) J Biol Chem. 2014 Aug 1;289(31):21782-94. (PMID: 24942740) Acta Crystallogr D Biol Crystallogr. 2001 Mar;57(Pt 3):401-11. (PMID: 11223517) Arch Biochem Biophys. 1974 Dec;165(2):691-708. (PMID: 4374138) Trends Microbiol. 2000 May;8(5):221-5. (PMID: 10785638) J Inorg Biochem. 2012 Feb;107(1):129-43. (PMID: 22204943) Nat Chem Biol. 2008 Mar;4(3):148-51. (PMID: 18277969) Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32. (PMID: 15572765) J Am Chem Soc. 2004 Mar 17;126(10):3081-90. (PMID: 15012137) J Inorg Biochem. 2012 Nov;116:55-62. (PMID: 23010330) Biochemistry. 2010 Nov 30;49(47):10117-30. (PMID: 20961038) Appl Environ Microbiol. 2016 Jan 15;82(6):1917-1923. (PMID: 26773085) J Magn Reson. 2006 Jan;178(1):42-55. (PMID: 16188474) Nucleic Acids Res. 2019 Jan 8;47(D1):D666-D677. (PMID: 30289528) J Biol Inorg Chem. 2014 Oct;19(7):1057-67. (PMID: 24722994) Trends Microbiol. 2010 Jun;18(6):266-74. (PMID: 20382024) Acta Crystallogr C. 2000 Jul;56 (Pt 7):786-8. (PMID: 10935080) FEMS Microbiol Rev. 2010 Jul;34(4):496-531. (PMID: 20236329) PLoS One. 2012;7(8):e43146. (PMID: 22916218) Inorg Chem. 2013 Feb 4;52(3):1584-90. (PMID: 23339443) |
معلومات مُعتمدة: | P30 CA060553 United States CA NCI NIH HHS; R01 GM111097 United States GM NIGMS NIH HHS; R35 GM118035 United States GM NIGMS NIH HHS; T32 GM008382 United States GM NIGMS NIH HHS |
فهرسة مساهمة: | Keywords: MbnP; copper; diheme peroxidase; kynurenine; methanotroph |
المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Metalloproteins) 343-65-7 (Kynurenine) 789U1901C5 (Copper) |
تواريخ الأحداث: | Date Created: 20210602 Date Completed: 20211130 Latest Revision: 20211201 |
رمز التحديث: | 20221213 |
مُعرف محوري في PubMed: | PMC8201829 |
DOI: | 10.1073/pnas.2100680118 |
PMID: | 34074779 |
قاعدة البيانات: | MEDLINE |
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