دورية أكاديمية
Validation of a Gradient Diffusion Method (Etest) for Testing of Antimicrobial Susceptibility of Aerococcus urinae to Fluoroquinolones.
| العنوان: | Validation of a Gradient Diffusion Method (Etest) for Testing of Antimicrobial Susceptibility of Aerococcus urinae to Fluoroquinolones. |
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| المؤلفون: | Roy FE; Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université Laval, Quebec, Canada., Berteau T; Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université Laval, Quebec, Canada., Bestman-Smith J; Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université Laval, Quebec, Canada.; Centre Hospitalier Universitaire de Québec-Université Laval, Quebec, Canada., Grandjean Lapierre S; Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université de Montréal, Montreal, Canada.; Immunopathology Axis, Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Canada.; Department of Laboratory Medicine, Centre Hospitalier de l'Université de Montréal, Montreal, Canada., Dufresne SF; Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université de Montréal, Montreal, Canada.; Department of Laboratory Medicine, Centre Hospitalier de l'Université de Montréal, Montreal, Canada.; Division of Infectious Diseases and Clinical Microbiology, Department of Medicine, Maisonneuve-Rosemont Hospital (CEMTL), Montreal, Canada., Domingo MC; Laboratoire de Santé Publique du Québec, Institut National de Santé Publique du Québec, Ste-Anne-de-Bellevue, Canada., Leduc JM; Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Université de Montréal, Montreal, Canada.; Department of Laboratory Medicine, Centre Hospitalier de l'Université de Montréal, Montreal, Canada.; Centre de Recherche du Centre Intégré Universitaire de Santé et Services Sociaux du Nord-de-l'Île-de-Montréal, Hôpital du Sacré-Coeur de Montréal, Montreal, Canada. |
| المصدر: | Journal of clinical microbiology [J Clin Microbiol] 2021 Jul 19; Vol. 59 (8), pp. e0025921. Date of Electronic Publication: 2021 Jul 19. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 7505564 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-660X (Electronic) Linking ISSN: 00951137 NLM ISO Abbreviation: J Clin Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, American Society for Microbiology. |
| مواضيع طبية MeSH: | Anti-Bacterial Agents*/pharmacology , Fluoroquinolones*/pharmacology, Aerococcus ; Animals ; Canada ; Disk Diffusion Antimicrobial Tests ; Microbial Sensitivity Tests ; Quebec ; Sheep |
| مستخلص: | Aerococcus urinae is a urinary pathogen with well-described resistance to fluoroquinolones. This study aimed to validate the gradient diffusion (GD) method (Etest) on cation-adjusted Mueller-Hinton agar with 5% sheep blood for testing the susceptibilities of Aerococcus urinae to the antimicrobial agents ciprofloxacin and levofloxacin and to compare the Etest to the broth microdilution (BMD) method from CLSI document M45-A3. Agar dilution (AD), as recommended by EUCAST, was used as an alternative reference method to arbitrate discrepancies or address technical issues. Aerococcus urinae isolates from urinary specimens were prospectively collected between June 2016 and December 2017 from six hospitals in Quebec, Canada, and identifications were confirmed using Vitek MS with the IVD 3.0 database. Of the 207 isolates tested using BMD, 37 (17.9%) showed trailing and 19 (9.2%) showed insufficient growth; these were tested using AD. Also, 38 isolates (18.4%) for ciprofloxacin and 13 isolates (6.3%) for levofloxacin showed a lack of essential or categorical agreement between the Etest and BMD and were also tested by AD. By use of a combined reference method (BMD or AD), the susceptibility rates of Aerococcus urinae were 82.6% and 81.6% for ciprofloxacin and levofloxacin, respectively. Categorical agreement between GD and the combined reference methods was 95.2% for ciprofloxacin and 97.1% for levofloxacin, with no very major error identified. Major and minor error rates were 0.6% and 4.3% for ciprofloxacin and 1.2% and 1.9% for levofloxacin. Overall, antimicrobial susceptibility testing (AST) using the Etest on sheep blood agar showed good agreement with the reference methods and can be considered by clinical laboratories wishing to perform AST on Aerococcus urinae isolates. |
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| فهرسة مساهمة: | Keywords: Aerococcus urinae; agar dilution; antimicrobial susceptibility testing; broth microdilution; ciprofloxacin; fluoroquinolones; gradient diffusion; levofloxacin; urinary tract infections |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Fluoroquinolones) |
| SCR Organism: | Aerococcus urinae |
| تواريخ الأحداث: | Date Created: 20210602 Date Completed: 20210728 Latest Revision: 20220120 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8373239 |
| DOI: | 10.1128/JCM.00259-21 |
| PMID: | 34076476 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-660X |
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| DOI: | 10.1128/JCM.00259-21 |