دورية أكاديمية
أعرض في EDS

Cytological and molecular diagnosis of Hürthle cell thyroid tumors: Analysis of three cases.

التفاصيل البيبلوغرافية
العنوان: Cytological and molecular diagnosis of Hürthle cell thyroid tumors: Analysis of three cases.
المؤلفون: Titov SE; Department of The Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology SB RAS, Novosibirsk 630090, Russia.; AO Vector-Best, Novosibirsk 630117, Russia., Poloz TL; Department of Cytopathology, Clinical Hospital RZD-Medicine, Novosibirsk 630003, Russia., Veryaskina YA; Department of The Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology SB RAS, Novosibirsk 630090, Russia.; Institute of Cytology and Genetics SB RAS, Novosibirsk 630090, Russia., Anishchenko VV; Department of Surgery, Novosibirsk State Medical University, Novosibirsk 630091, Russia.
المصدر: Molecular and clinical oncology [Mol Clin Oncol] 2021 Jul; Vol. 15 (1), pp. 149. Date of Electronic Publication: 2021 May 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Spandidos Publications UK Ltd Country of Publication: England NLM ID: 101613422 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2049-9450 (Print) Linking ISSN: 20499450 NLM ISO Abbreviation: Mol Clin Oncol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: London, UK : Spandidos Publications UK Ltd
مستخلص: The cytological diagnosis of Hürthle cell (oncocytic) thyroid tumors by means of fine-needle aspiration biopsy represents a challenge, as Hürthle cell polymorphism and atypia alone are not indications of malignancy. In our recent work, an original diagnostic algorithm was proposed, which identified and typed malignant thyroid tumors by analyzing the molecular markers of cytological preparations. The aim of the present study was to assess the effectiveness of this algorithm at detecting Hürthle cell thyroid tumors in clinical samples used for cytological examination. Cytological and histological examinations of the biopsy material were performed for three patients with nodular neoplasms. Biopsy material of these patients was analyzed by quantitative PCR using preselected molecular markers [normalized concentrations of High-mobility group AT-hook 2 mRNA, three microRNAs (miRNAs or miRs; miR-146b, miR-221 and miR-375) and the mitochondrial (mtDNA)/nuclear DNA ratio]. The results revealed that the molecular test determined the malignancy of three cases of Hürthle cell tumor. This method may therefore be used to complement the cytological diagnosis of fine-needle aspiration biopsy. In all three cases, there was an increased content of mtDNA, indicating Hürthle cell malignancies. Furthermore, in the first case [Hürthle cell carcinoma (HCC)], increased miRNA-221 content was detected, which also indicated malignancy. In the second case (Hürthle cell papillary thyroid carcinoma), an increased level of miRNA-146b was present, which indicated papillary carcinoma. In the third case (Hürthle cell adenoma), no markers of malignancy were identified. The present study demonstrated that molecular testing together with cytological analysis can reduce the isk of error in the preoperative cytological diagnosis of unclear or ambivalent cases.
Competing Interests: The authors declare that they have no competing interests.
(Copyright: © Titov et al.)
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فهرسة مساهمة: Keywords: Hürthle cell; fine-needle biopsy; microRNA; mitochondrial DNA; thyroid gland
تواريخ الأحداث: Date Created: 20210607 Latest Revision: 20210608
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC8165692
DOI: 10.3892/mco.2021.2311
PMID: 34094547
قاعدة البيانات: MEDLINE
الوصف
تدمد:2049-9450
DOI:10.3892/mco.2021.2311