دورية أكاديمية
Histidine-Rich Glycoprotein Functions as a Dual Regulator of Neutrophil Activity in Horses.
| العنوان: | Histidine-Rich Glycoprotein Functions as a Dual Regulator of Neutrophil Activity in Horses. |
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| المؤلفون: | Muko R; Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Division of Animal Life Science, Institute of Agriculture, Tokyo, Japan., Matsuda H; Laboratory of Comparative Animal Medicine, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Oikawa MA; Diagnostic and Research Laboratory, Equine Veterinary Medical Center, Education City, Doha, Qatar., Shin T; Department of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, South Korea., Matsuda K; Laboratory of Comparative Animal Medicine, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Sato H; Ritto Training Center, Japan Racing Association, Shiga, Japan., Sunouchi T; Laboratory of Comparative Animal Medicine, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan., Tanaka A; Cooperative Major in Advanced Health Science, Graduate School of Bio-Applications and System Engineering, Tokyo University of Agriculture and Technology, Division of Animal Life Science, Institute of Agriculture, Tokyo, Japan; Laboratory of Comparative Animal Medicine, Division of Animal Life Science, Institute of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan. Electronic address: akane@cc.tuat.ac.jp. |
| المصدر: | Journal of equine veterinary science [J Equine Vet Sci] 2021 Jul; Vol. 102, pp. 103620. Date of Electronic Publication: 2021 Apr 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 8216840 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 0737-0806 (Print) Linking ISSN: 07370806 NLM ISO Abbreviation: J Equine Vet Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2003- : New York : Elsevier Original Publication: [Wildomar, CA : William E. Jones, c1981- |
| مواضيع طبية MeSH: | Horse Diseases* , Neutrophils*, Animals ; Chemotaxis, Leukocyte ; Horses ; Humans ; Proteins ; Systemic Inflammatory Response Syndrome/veterinary |
| مستخلص: | Histidine-rich glycoprotein (HRG) is an abundant plasma protein that has been identified in most mammals. We first identified whole genome sequence of equine HRG (eHRG) and succeeded to purify eHRG from plasma of horses. Since HRG interacts with various ligands, this protein is thought to be involved in immune response, coagulation, and angiogenesis. Systemic inflammatory response syndrome (SIRS) is characterized as a non-specific, clinical, pro-inflammatory immune response that damage organs and tissues in the host. Recent reports revealed that blood HRG levels in human patients with SIRS are approximately 50% lower than those in healthy controls, indicating the use of HRG as a biomarker or treatment for SIRS. SIRS is also a serious issue in equine medicine. In this study, we investigated various effects of eHRG on neutrophil functions, including adhesion, migration, phagocytosis, reactive oxygen species (ROS) production, and lysosome maturation using neutrophils isolated from horses. Microscopic observation showed that the addition of eHRG to the culture diminished adhesion of neutrophils stimulated with LPS. Using the Boyden chamber technique, we showed that eHRG reduced neutrophil chemotaxis induced by recombinant human IL-8. Luminol-dependent chemiluminescence assay demonstrated that eHRG restrained the peak of LPS-promoted ROS production from neutrophils. In contrast, eHRG promoted phagocytic activity evaluated with uptake of fluorescent dye conjugated particles, as well as lysosomal maturation assessed using fluorescent staining for lysosomes of equine neutrophils. These results indicated that eHRG acts as a dual regulator of neutrophils in horses. (Copyright © 2021. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Histidine-rich glycoprotein; Horses; Neutrophils; Reactive oxygen species; Systemic inflammatory response syndrome |
| المشرفين على المادة: | 0 (Proteins) 0 (histidine-rich proteins) |
| تواريخ الأحداث: | Date Created: 20210613 Date Completed: 20210709 Latest Revision: 20210709 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jevs.2021.103620 |
| PMID: | 34119191 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0737-0806 |
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| DOI: | 10.1016/j.jevs.2021.103620 |