دورية أكاديمية
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Phenazines as model low-midpoint potential electron shuttles for photosynthetic bioelectrochemical systems.

التفاصيل البيبلوغرافية
العنوان: Phenazines as model low-midpoint potential electron shuttles for photosynthetic bioelectrochemical systems.
المؤلفون: Clifford ER; Department of Chemistry, University of Cambridge Lensfield Road Cambridge CB2 1EW UK jz366@cam.ac.uk., Bradley RW; Department of Life Sciences Sir Alexander Fleming Building, Imperial College SW7 2AZ UK., Wey LT; Department of Biochemistry, University of Cambridge Tennis Court Road Cambridge CB2 1QW UK ch26@cam.ac.uk., Lawrence JM; Department of Biochemistry, University of Cambridge Tennis Court Road Cambridge CB2 1QW UK ch26@cam.ac.uk., Chen X; Department of Chemistry, University of Cambridge Lensfield Road Cambridge CB2 1EW UK jz366@cam.ac.uk., Howe CJ; Department of Biochemistry, University of Cambridge Tennis Court Road Cambridge CB2 1QW UK ch26@cam.ac.uk., Zhang JZ; Department of Chemistry, University of Cambridge Lensfield Road Cambridge CB2 1EW UK jz366@cam.ac.uk.
المصدر: Chemical science [Chem Sci] 2021 Jan 15; Vol. 12 (9), pp. 3328-3338. Date of Electronic Publication: 2021 Jan 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101545951 Publication Model: Electronic Cited Medium: Print ISSN: 2041-6520 (Print) Linking ISSN: 20416520 NLM ISO Abbreviation: Chem Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : Royal Society of Chemistry, [2010]-
مستخلص: Bioelectrochemical approaches for energy conversion rely on efficient wiring of natural electron transport chains to electrodes. However, state-of-the-art exogenous electron mediators give rise to significant energy losses and, in the case of living systems, long-term cytotoxicity. Here, we explored new selection criteria for exogenous electron mediation by examining phenazines as novel low-midpoint potential molecules for wiring the photosynthetic electron transport chain of the cyanobacterium Synechocystis sp. PCC 6803 to electrodes. We identified pyocyanin (PYO) as an effective cell-permeable phenazine that can harvest electrons from highly reducing points of photosynthesis. PYO-mediated photocurrents were observed to be 4-fold higher than mediator-free systems with an energetic gain of 200 mV compared to the common high-midpoint potential mediator 2,6-dichloro-1,4-benzoquinone (DCBQ). The low-midpoint potential of PYO led to O 2 reduction side-reactions, which competed significantly against photocurrent generation; the tuning of mediator concentration was important for outcompeting the side-reactions whilst avoiding acute cytotoxicity. DCBQ-mediated photocurrents were generally much higher but also decayed rapidly and were non-recoverable with fresh mediator addition. This suggests that the cells can acquire DCBQ-resistance over time. In contrast, PYO gave rise to steadier current enhancement despite the co-generation of undesirable reactive oxygen species, and PYO-exposed cells did not develop acquired resistance. Moreover, we demonstrated that the cyanobacteria can be genetically engineered to produce PYO endogenously to improve long-term prospects. Overall, this study established that energetic gains can be achieved via the use of low-potential phenazines in photosynthetic bioelectrochemical systems, and quantifies the factors and trade-offs that determine efficacious mediation in living bioelectrochemical systems.
Competing Interests: There are no conflicts to declare.
(This journal is © The Royal Society of Chemistry.)
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معلومات مُعتمدة: BB/M011194/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
تواريخ الأحداث: Date Created: 20210624 Latest Revision: 20231111
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC8179378
DOI: 10.1039/d0sc05655c
PMID: 34164103
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-6520
DOI:10.1039/d0sc05655c