دورية أكاديمية
Genomic analysis of carbapenem-resistant Pseudomonas aeruginosa ST143 clone showing susceptibility to broad-spectrum cephalosporins.
| العنوان: | Genomic analysis of carbapenem-resistant Pseudomonas aeruginosa ST143 clone showing susceptibility to broad-spectrum cephalosporins. |
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| المؤلفون: | Streling AP; Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine-Escola Paulista de Medicina (EPM), São Paulo - SP, Brazil. Electronic address: anastreling@gmail.com., Cayô R; Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine-Escola Paulista de Medicina (EPM), São Paulo - SP, Brazil; Universidade Federal de São Paulo (UNIFESP), Laboratório de Imunologia e Microbiologia (LIB), Setor de Biologia Molecular, Microbiologia e Imunologia, Departamento de Ciências Biológicas (DCB), Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF), Diadema - SP, Brazil., Nodari CS; Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine-Escola Paulista de Medicina (EPM), São Paulo - SP, Brazil., Almeida LGP; National Laboratory for Scientific Computing (LNCC), Petrópolis - RJ, Brazil., Santos FF; Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine-Escola Paulista de Medicina (EPM), São Paulo - SP, Brazil., Hanson B; Center for Antimicrobial Resistance and Microbial Genomics, University of Texas Health Science Center, Houston, TX, USA; Division of Infectious Diseases, and Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, McGovern School of Medicine, Houston, TX, USA; Center for Infectious Diseases, University of Texas Health Science Center, School of Public Health, Houston, TX, USA., Dinh AQ; Center for Antimicrobial Resistance and Microbial Genomics, University of Texas Health Science Center, Houston, TX, USA; Division of Infectious Diseases, and Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, McGovern School of Medicine, Houston, TX, USA., Vasconcelos ATR; National Laboratory for Scientific Computing (LNCC), Petrópolis - RJ, Brazil., Miller WR; Center for Antimicrobial Resistance and Microbial Genomics, University of Texas Health Science Center, Houston, TX, USA; Division of Infectious Diseases, and Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, McGovern School of Medicine, Houston, TX, USA; Center for Infectious Diseases, University of Texas Health Science Center, School of Public Health, Houston, TX, USA., Arias CA; Center for Antimicrobial Resistance and Microbial Genomics, University of Texas Health Science Center, Houston, TX, USA; Division of Infectious Diseases, and Department of Pathology and Laboratory Medicine, University of Texas Health Science Center, McGovern School of Medicine, Houston, TX, USA; Center for Infectious Diseases, University of Texas Health Science Center, School of Public Health, Houston, TX, USA; MD Anderson Cancer Center and UTHealth Graduate School of Biomedical Sciences, Houston, TX, USA., Gales AC; Universidade Federal de São Paulo (UNIFESP), Laboratório Alerta, Division of Infectious Diseases, Department of Internal Medicine-Escola Paulista de Medicina (EPM), São Paulo - SP, Brazil. |
| المصدر: | Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2021 Sep; Vol. 26, pp. 177-179. Date of Electronic Publication: 2021 Jun 24. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer Country of Publication: Netherlands NLM ID: 101622459 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-7173 (Electronic) Linking ISSN: 22137165 NLM ISO Abbreviation: J Glob Antimicrob Resist Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer |
| مواضيع طبية MeSH: | Cephalosporins*/pharmacology , Pseudomonas aeruginosa*/genetics, Anti-Bacterial Agents/pharmacology ; Clone Cells ; Genomics ; Meropenem ; Microbial Sensitivity Tests |
| مستخلص: | Objectives: Using whole-genome sequencing (WGS), we aimed to characterise a Pseudomonas aeruginosa ST143 clinical strain (Pb9) that presented resistance to meropenem and imipenem and susceptibility to piperacillin/tazobactam and broad-spectrum cephalosporins. Methods: The antimicrobial susceptibility profile was confirmed by broth microdilution. WGS was performed using an Illumina MiSeq platform to identify possible genetic determinants of β-lactam resistance. Transcription levels of chromosomally encoded efflux systems and oprD were evaluated by RT-qPCR. Results: WGS analysis showed that no acquired carbapenemase-encoding gene was found in isolate Pb9, although mutations in the chromosomally encoded β-lactamase genes bla Conclusion: Our results suggest that the β-lactam resistance phenotype presented by strain Pb9 might be related to an association of OprD loss with hyperexpression of the efflux pump systems MexAB-OprM and MexEF-OprN. However, the contribution of OXA-488, PDC-5 and PIB-1 to this phenotype remains unclear and warrants further investigation. (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Carbapenem resistance; Genome; Non-fermenting Gram-negative bacilli; OXA-50; PDC-5; Pseudomonas |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Cephalosporins) FV9J3JU8B1 (Meropenem) |
| تواريخ الأحداث: | Date Created: 20210627 Date Completed: 20211025 Latest Revision: 20220524 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jgar.2021.05.019 |
| PMID: | 34175444 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 2213-7173 |
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| DOI: | 10.1016/j.jgar.2021.05.019 |